Acidification by nitrogen metabolism triggers extracellular biopolymer production in an oleaginous yeast DOI Creative Commons
Henrique Sepúlveda Del Rio Hamacek,

Oksana Tingajeva,

Katharina Ostertag

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Май 4, 2025

Abstract The oleaginous yeast Rhodotorula toruloides is a natural producer of lipids and carotenoids. However, its potential as extracellular biopolymers remains unexplored. Hence, we aimed to evaluate the R. CCT0783 for biopolymer production. We found that carbon-to-nitrogen ratio influenced exopolysaccharide (EPS) production, reaching 5.84 ± 0.45 g/L under glucose-rich conditions. characterized crude using FTIR GC-MS, identifying polysaccharide peaks, wherein EPS consisted (%) glucose (88.83 4.87), galactose (5.50 1.50), mannose (4.80 1.57), xylose arabinose (0.87 0.04) monomers. dried also contained fractional presence protein (1.0%). Interestingly, inorganic, but not organic, nitrogen metabolism was associated with acidification culture environment simultaneous A comparison between non-buffered buffered media indicated produced when final pH around or below 2, which observed in former latter. Finally, comparative bioinformatics analysis allowed us map putative biosynthesis transport pathways, well regulators intracellular maintenance. In conclusion, our study demonstrates toruloides’ an microbial producer, enabling development cell factory. Importance Microbial are extensively investigated their impact on health. developing biotechnology process producing such challenging despite valuable applications. Considering this opportunity, biopolymers. Our describes conditions . This could have wide range applications, from gelling agents pharmaceuticals emulsifiers food industry. Furthermore, results be useful metabolic engineering factories.

Язык: Английский

Acidification by nitrogen metabolism triggers extracellular biopolymer production in an oleaginous yeast DOI Creative Commons
Henrique Sepúlveda Del Rio Hamacek,

Oksana Tingajeva,

Katharina Ostertag

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Май 4, 2025

Abstract The oleaginous yeast Rhodotorula toruloides is a natural producer of lipids and carotenoids. However, its potential as extracellular biopolymers remains unexplored. Hence, we aimed to evaluate the R. CCT0783 for biopolymer production. We found that carbon-to-nitrogen ratio influenced exopolysaccharide (EPS) production, reaching 5.84 ± 0.45 g/L under glucose-rich conditions. characterized crude using FTIR GC-MS, identifying polysaccharide peaks, wherein EPS consisted (%) glucose (88.83 4.87), galactose (5.50 1.50), mannose (4.80 1.57), xylose arabinose (0.87 0.04) monomers. dried also contained fractional presence protein (1.0%). Interestingly, inorganic, but not organic, nitrogen metabolism was associated with acidification culture environment simultaneous A comparison between non-buffered buffered media indicated produced when final pH around or below 2, which observed in former latter. Finally, comparative bioinformatics analysis allowed us map putative biosynthesis transport pathways, well regulators intracellular maintenance. In conclusion, our study demonstrates toruloides’ an microbial producer, enabling development cell factory. Importance Microbial are extensively investigated their impact on health. developing biotechnology process producing such challenging despite valuable applications. Considering this opportunity, biopolymers. Our describes conditions . This could have wide range applications, from gelling agents pharmaceuticals emulsifiers food industry. Furthermore, results be useful metabolic engineering factories.

Язык: Английский

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