PeerJ,
Год журнала:
2024,
Номер
12, С. e18313 - e18313
Опубликована: Окт. 30, 2024
The
ongoing
high
occurrence
of
harmful
infectious
diseases
significantly
threatens
human
health.
Existing
methods
used
to
control
such
primarily
involve
targeting
the
pathogens,
usually
neglecting
vital
role
host
factors
in
disease
advancement.
Gamma
delta
(γδ)
T
cells
act
as
a
bridge
between
innate
and
adaptive
immunity,
playing
crucial
combating
pathogen
invasion.
Among
these
γδT
cell
subsets,
which
are
categorized
based
on
receptor
variable
expression
patterns,
V
(δ)
1
Microorganisms,
Год журнала:
2025,
Номер
13(2), С. 311 - 311
Опубликована: Янв. 31, 2025
The
emergence
and
evolution
of
SARS-CoV-2
variants,
such
as
Delta
Omicron,
pose
significant
challenges
to
pandemic
management.
This
study
evaluated
the
effectiveness
reverse-transcription
polymerase
chain
reaction
(RT-PCR)
whole-genome
sequencing
(WGS)
in
detecting
characterizing
variants
using
624
samples
collected
South
Korea
from
mid-2021
mid-2022.
Two
RT-PCR
genotyping
assays
demonstrated
a
high
concordance
rate
(90.4%)
identifying
variant
during
its
dominance.
In
contrast,
WGS
revealed
extensive
genetic
diversity
among
Omicron
sub-lineages,
29
distinct
including
two
Korea-specific
(BA.1.1.5
BA.2.3.8).
Clustering
analysis
data
highlighted
groupings
BA.1,
BA.2,
BA.5
with
overlap
shared
mutations
suggesting
evolutionary
convergence.
Sub-lineage
expanded
rapid
transmission
phases
subsequently
consolidated
dominant
lineages
emerged.
These
findings
highlight
complementary
strengths
underscore
importance
integrating
these
methodologies
for
effective
monitoring
public
health
response.
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(2), С. 670 - 670
Опубликована: Янв. 14, 2025
With
the
aim
of
developing
novel
anti-SARS-CoV-2
drugs
to
address
ongoing
evolution
and
emergence
drug-resistant
strains,
reported
SARS-CoV-2
Mpro
inhibitor
WU-04
was
selected
as
a
lead
find
novel,
highly
potent,
broad-spectrum
inhibitors.
Using
fragment-based
multilevel
virtual
screening
strategy,
15
hit
compounds
were
identified
subsequently
synthesized.
Among
them,
A5
(IC50
=
1.05
μM),
A6
1.08
A9
0.154
μM)
demonstrated
potent
inhibition
comparable
or
slightly
weaker
than
WU-04.
Antiviral
activity
evaluations
revealed
that
compound
exhibited
strongest
antiviral
with
an
EC50
value
0.18
μM,
quite
marketed
drug
Nirmatrelvir
(EC50
0.123
inferior
0.042
μM).
Molecular
dynamics
simulations
elucidated
key
interactions
between
A5,
A6,
A9,
binding
pocket
Mpro,
providing
valuable
insights
into
their
mechanisms
action.
These
findings
identify
promising
for
development.
Healthcare,
Год журнала:
2025,
Номер
13(3), С. 344 - 344
Опубликована: Фев. 6, 2025
Background/objectives:
Attitudes
towards
COVID-19
vaccination
vary
globally,
influenced
by
political
and
cultural
factors.
This
research
aimed
to
assess
the
views
of
people
without
a
healthcare
qualification
in
Europe
on
safety,
effectiveness,
necessity
as
well
how
informed
they
felt.
The
secondary
outcomes
focused
respondents’
were
affected
demographic
context
factors
included
comparison
country
level
feeling
informed.
Methods:
A
mixed-method
cross-sectional
online
survey
eight
European
countries,
using
convenience
sampling.
Results:
total
1008
adults
completed
survey,
60%
whom
female.
While
only
44.1%
considered
vaccines
safe,
43.5%
effective,
44.9%
necessary,
80.0%
had
been
vaccinated.
Four
ten
strongly
agreed
that
informed,
while
over
quarter
did
not
answer
question.
Younger
respondents,
well-informed
individuals,
German
respondents
more
inclined
perceive
both
effective
necessary.
Conclusions:
Motivations
for
perceived
health
social
benefits,
concerns
preference
“natural
immunity”,
rapid
development
vaccine,
potential
unknown
long-term
effects.
correlation
existed
between
about
different
their
likelihood
having
Disease
X
stands
as
a
symbol
for
subset
of
emerging
infectious
diseases
rising
to
prominence
significant
challenge
global
health
security.
This
comprehensive
review
delves
into
the
historical
context,
precise
definition,
and
illustrative
case
studies
disease
X,
including
notable
examples,
such
COVID-19,
SARS,
Ebola.
The
discussion
then
transitions
an
examination
potential
origins
with
particular
focus
on
zoonotic
pathways
influence
environmental
factors.
concludes
suite
proposed
strategies
aimed
at
prevention
containment
emphasizing
critical
role
vigilant
surveillance,
swift
response
mechanisms,
necessity
fostering
international
cooperation.
Applied Microbiology and Biotechnology,
Год журнала:
2024,
Номер
108(1)
Опубликована: Окт. 16, 2024
Our
previous
study
has
found
that
monoclonal
antibodies
targeting
a
conserved
epitope
peptide
spanning
from
residues
1144
to
1156
of
SARS-CoV-2
spike
(S)
protein,
namely
S(1144-1156),
can
broadly
neutralize
all
the
prevalent
strains,
including
wild
type,
Alpha,
Epsilon,
Delta,
and
Gamma
variants.
In
study,
S(1144-1156)
was
conjugated
with
bovine
serum
albumin
(BSA)
formulated
Montanide
ISA
51
adjuvant
for
inoculation
in
BALB/c
mice
its
potential
as
vaccine
candidate.
Results
showed
titers
S
protein-specific
IgGs
neutralizing
mouse
sera
against
various
variants,
Omicron
sublineages,
were
largely
induced
along
three
doses
immunization.
The
significant
release
IFN-γ
IL-2
also
observed
by
ELISpot
assays
through
stimulating
vaccinated
splenocytes
peptide.
Furthermore,
vaccination
S(1143-1157)-
S(1142-1158)-EGFP
fusion
proteins
elicit
more
than
S(1144-1156)-EGFP
protein.
Interestingly,
antisera
collected
inoculated
exhibited
better
efficacy
BA.2.86
JN.1
subvariants
BA.1,
BA.2,
XBB
subvariants.
Since
amino
acid
sequences
are
highly
among
immunogen
containing
core
be
designed
effective
COVID-19
vaccine.
KEY
POINTS:
•
Inoculation
induce
bnAbs
stimulated
splenocytes.
S(1143-1157)
S(1142-1158)
vaccines
nAbs
mice.
