An optimized protocol for routine development of cell culture from adult oyster, Crassostrea madrasensis DOI
Soumya Balakrishnan,

Ambadi Kannan Maliyekkal Sajeevan,

S. Parvathi

и другие.

Cell Biology International, Год журнала: 2024, Номер 48(7), С. 923 - 938

Опубликована: Март 27, 2024

Abstract Marine molluscan cell lines, required for virus screening and cultivation, form essential tools developing health management strategies these animals in the blue economy. Moreover, they are also crucial to develop cultivated seafood. As there is no valid marine line, primary cultures relied upon all investigations. A sound protocol generating from molluscs entailed, but existing protocols often involve heavy antibiotic usage depuration that invariably affect gene expression health. This work presents an easy‐to‐adopt, time‐saving using non‐depurated mollusc Crassostrea madrasensis , which requires only initial treatment minimal exposure or use of antibiotics culture medium. The important experimental considerations arriving at this have been elucidated. Accordingly, sodium hypochlorite neomycin sulfate were chosen disinfecting tissues. study first shrimp medium (SCCM) as a culture. Despite being osmoconformers, oysters exhibited stable intracellular osmotic conditions pH, which, when provided vitro, promoted effective cardiomyocyte formation. viability could be enhanced 10% fetal bovine serum (FBS), healthy obtained SCCM without FBS. optimized allowed regular beating clusters retained month. Limited proliferation, shown by BrdU assay, demands further interventions, such possibly producing induced pluripotent stem cells. align with some requirements meat molluscs.

Язык: Английский

Current state of marine plastic pollution and its technology for more eminent evidence: A review DOI
Setyo Budi Kurniawan, Siti Rozaimah Sheikh Abdullah, Muhammad Fauzul Imron

и другие.

Journal of Cleaner Production, Год журнала: 2020, Номер 278, С. 123537 - 123537

Опубликована: Авг. 5, 2020

Язык: Английский

Процитировано

86
Seasonal variation of plastic debris accumulation in the estuary of Wonorejo River, Surabaya, Indonesia DOI
Setyo Budi Kurniawan, Muhammad Fauzul Imron

Environmental Technology & Innovation, Год журнала: 2019, Номер 16, С. 100490 - 100490

Опубликована: Сен. 18, 2019

Язык: Английский

Процитировано

82
Learning from regeneration research organisms: The circuitous road to scar free wound healing DOI Creative Commons
Jami R. Erickson, Karen Echeverri

Developmental Biology, Год журнала: 2017, Номер 433(2), С. 144 - 154

Опубликована: Ноя. 25, 2017

Язык: Английский

Процитировано

83
Myticalins: A Novel Multigenic Family of Linear, Cationic Antimicrobial Peptides from Marine Mussels (Mytilus spp.) DOI Creative Commons
Gabriele Leoni, Andrea Poli, Mario Mardirossian

и другие.

Marine Drugs, Год журнала: 2017, Номер 15(8), С. 261 - 261

Опубликована: Авг. 22, 2017

The application of high-throughput sequencing technologies to non-model organisms has brought new opportunities for the identification bioactive peptides from genomes and transcriptomes. From this point view, marine invertebrates represent a potentially rich, yet largely unexplored resource de novo discovery due their adaptation diverse challenging habitats. Bioinformatics analyses available genomic transcriptomic data allowed us identify myticalins, novel family antimicrobial (AMPs) mussel Mytilus galloprovincialis, similar AMPs Modiolus spp., named modiocalins. Their coding sequence encompasses two conserved N-terminal (signal peptide) C-terminal (propeptide) regions hypervariable central cationic region corresponding mature peptide. Myticalins are taxonomically restricted Mytiloida they can be classified into four subfamilies. These subject considerable interindividual variability possibly presence/absence variation. Functional assays performed on selected members indicate remarkable tissue-specific expression (in gills) broad spectrum activity against both Gram-positive Gram-negative bacteria. Overall, we present first linear ever described in mussels confirm great potential bioinformatics tools organisms.

Язык: Английский

Процитировано

65
Functional plasticity in oyster gut microbiomes along a eutrophication gradient in an urbanized estuary DOI Creative Commons
Rebecca J. Stevick,

Anton F. Post,

Marta Gomez‐Chiarri

и другие.

Animal Microbiome, Год журнала: 2021, Номер 3(1)

Опубликована: Янв. 6, 2021

Abstract Background Oysters in coastal environments are subject to fluctuating environmental conditions that may impact the ecosystem services they provide. Oyster-associated microbiomes responsible for some of these services, particularly nutrient cycling benthic habitats. The effects climate change on host-associated microbiome composition well-known, but functional changes and how host physiology functioning poorly characterized. We investigated parameters affect oyster-associated microbial community structure function along a trophic gradient Narragansett Bay, Rhode Island, USA. Adult eastern oyster, Crassostrea virginica, gut seawater samples were collected at 5 sites this estuarine August 2017. Samples analyzed by 16S rRNA gene sequencing characterize bacterial structures metatranscriptomes sequenced determine oyster responses local environments. Results There significant differences between water samples, suggesting selection certain taxa host. Increasing salinity, pH, dissolved oxygen, decreasing nitrate, nitrite phosphate concentrations observed North South gradient. Transcriptionally active similar different sites, expression genes involved (nitrogen phosphorus) varied throughout reflecting regimes prevailing conditions. Conclusions shifts inform their services. As acidification expected increase due combined eutrophication, pollution, change, it is important relationships health, structure, communities.

Язык: Английский

Процитировано

39
Microbiome Analysis Reveals Diversity and Function of Mollicutes Associated with the Eastern Oyster, Crassostrea virginica DOI
Zachary T. Pimentel, Keith Dufault‐Thompson,

Kayla T. Russo

и другие.

mSphere, Год журнала: 2021, Номер 6(3)

Опубликована: Май 12, 2021

Marine invertebrate microbiomes play important roles in diverse host and ecological processes. However, a mechanistic understanding of host-microbe interactions is currently available for small number model organisms. Here, an integrated taxonomic functional analysis the microbiome eastern oyster, Crassostrea virginica, was performed using 16S rRNA gene-based amplicon profiling, shotgun metagenomics, genome-scale metabolic reconstruction. Relatively high variability observed across individual oysters among different tissue types. Specifically, significantly higher alpha diversity inner shell than gut, gill, mantle, pallial fluid samples, distinct composition revealed gut compared to other tissues examined this study. Targeted metagenomic sequencing microbiota led further characterization dominant bacterial taxon, class Mollicutes, which captured by reconstruction metagenome-assembled genome (MAG). Genome-scale oyster Mollicutes MAG reduced set functions reliance on uptake host-derived nutrients. A chitin degradation arginine deiminase pathway were unique closely related genomes isolates, indicating mechanisms carbon energy acquisition oyster-associated systematic reanalysis public oyster-derived data prevalence adult guts lower relative abundance larvae biodeposits.IMPORTANCE Despite their biological significance, function frequently missing from many nonmodel marine invertebrates. As initial step toward filling gap study provides samples coastal salt pond August 2017. The identified types oysters, with some taxa showing specific tissues. comparative biodeposit, larva microbiomes. Phylogenomic suggested but functionally isolated To best our knowledge, represents first metagenomics-derived inference microbiome.

Язык: Английский

Процитировано

34
Unveiling the hidden threat: Molecular, cellular and behavioral effects of dietborne inorganic mercury and methylmercury in Dreissena polymorpha DOI Creative Commons

Clarisse Seguin,

Alice Marant,

Séverine Palacios-Paris

и другие.

Chemosphere, Год журнала: 2025, Номер 376, С. 144306 - 144306

Опубликована: Март 14, 2025

Язык: Английский

Процитировано

0
A Needle in A Haystack: Tracing Bivalve-Associated Viruses in High-Throughput Transcriptomic Data DOI Creative Commons
Umberto Rosani,

Maxwell Shapiro,

Paola Venier

и другие.

Viruses, Год журнала: 2019, Номер 11(3), С. 205 - 205

Опубликована: Март 1, 2019

Bivalve mollusks thrive in environments rich microorganisms, such as estuarine and coastal waters, they tend to accumulate various particles, including viruses. However, the current knowledge on mollusk viruses is mainly centered few pathogenic viruses, whereas a general view of bivalve-associated viromes lacking. This study was designed explore viral abundance diversity bivalve using transcriptomic datasets. From analyzing RNA-seq data 58 species, we have reconstructed 26 nearly complete over 413 partial RNA virus genomes. Although 96.4% predicted proteins refer new some sequences belong associated with species or other marine invertebrates. We considered short non-coding RNAs (sncRNA) post-transcriptional modifications occurring specifically tools for host-assignment. could not identify virus-derived small sncRNA reads obtained from oyster sample richest reads. Single Nucleotide Polymorphism (SNP) analysis revealed 938 A-to-G substitutions identified preferentially impacting AA di-nucleotide motif. Under-representation that motif under-represented these These findings improve our understanding viromes, set stage targeted investigations specificity dynamics

Язык: Английский

Процитировано

41
Hemolymph of molluscan origin: from biochemistry to modern biomaterials science DOI Creative Commons
Tomasz Machałowski, Teofil Jesionowski

Applied Physics A, Год журнала: 2020, Номер 127(1)

Опубликована: Дек. 10, 2020

Abstract Molluscan hemolymph is a unique kind of body fluid, which in many respects analogous to human blood, although there are several crucial differences. Here, for the first time, we critically analyze prospects applications this fluid modern biomaterials science. Particular attention paid biochemistry and chemistry molluscan hemolymph, as well hemocytes hemocyanins key functional players within biological fluid. We focus on multifunctional hemolytic cells involved immune response, especially biomineralization process. The next part review contains discussion shell formation regeneration from different points view. Finally, consider challenges, solutions, future directions application bioinspired material biomedicine.

Язык: Английский

Процитировано

35
Mobilizing molluscan models and genomes in biology DOI Creative Commons
Angus Davison, Maurine Neiman

Philosophical Transactions of the Royal Society B Biological Sciences, Год журнала: 2021, Номер 376(1825), С. 20200163 - 20200163

Опубликована: Апрель 4, 2021

Molluscs are among the most ancient, diverse, and important of all animal taxa. Even so, no individual mollusc species has emerged as a broadly applied model system in biology. We here make case that both perceptual methodological barriers have played role relative neglect molluscs research organisms. then summarize current application potential their genomes to address questions biology, state field when it comes availability resources such genome assemblies, cell lines, other key elements necessary mobilising development molluscan systems. conclude by contending cohesive community works together elevate multiple systems 'model' status will create new opportunities addressing basic biological problems, including general features evolution. This article is part Theo Murphy meeting issue 'Molluscan genomics: broad insights future directions for neglected phylum'.

Язык: Английский

Процитировано

31