Gene Reports, Год журнала: 2023, Номер 33, С. 101853 - 101853
Опубликована: Ноя. 4, 2023
Язык: Английский
Philosophical Transactions of the Royal Society B Biological Sciences, Год журнала: 2023, Номер 378(1882)
Опубликована: Июнь 12, 2023
Amphibians are at the forefront of bridging evolutionary gap between mammals and more ancient, jawed vertebrates. Currently, several diseases have targeted amphibians understanding their immune system has importance beyond use as a research model. The African clawed frog, Xenopus laevis , that is well conserved. We know features adaptive innate very similar for both, including existence B cells, T cells innate-like cells. In particular, study early stages development benefitted by studying X. tadpoles. tadpoles mainly rely on mechanisms pre-set or until after metamorphosis. this review we lay out what known about lymphoid organs how other amphibian systems different. Furthermore, will describe responds to some viral, bacterial fungal insults. This article part theme issue ‘Amphibian immunity: stress, disease ecoimmunology’.
Язык: Английский
Процитировано
23
Biology, Год журнала: 2023, Номер 12(6), С. 890 - 890
Опубликована: Июнь 20, 2023
In vitro systems have been mainly promoted by authorities to sustain research following the 3Rs principle, but continuously increasing amounts of evidence point out that in vivo experimentation is also extreme relevance. Xenopus laevis, an anuran amphibian, a significant model organism study evolutionary developmental biology, toxicology, ethology, neurobiology, endocrinology, immunology and tumor biology; thanks recent development genome editing, it has acquired relevant position field genetics. For these reasons, X. laevis appears be powerful alternative zebrafish for environmental biomedical studies. Its life cycle, as well possibility obtain gametes from adults during whole year embryos fertilization, allows experimental studies several biological endpoints, such gametogenesis, embryogenesis, larval growth, metamorphosis and, course, young adult stages. Moreover, with respect invertebrate even vertebrate animal models, displays higher degree similarity mammals. Here, we reviewed main available literature on use biosciences inspired Feymann’s revised view, “Plenty room biology at bottom,” suggest very useful all possible
Язык: Английский
Процитировано
12
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Июль 1, 2024
Amphibians represent a diverse group of tetrapods, marked by deep divergence times between their three systematic orders and families. Studying amphibian biology through the genomics lens increases our understanding features this animal class that other terrestrial vertebrates. The need for genomic resources is more urgent than ever due to increasing threats group. are one most imperiled taxonomic groups, with approximately 41% species threatened extinction habitat loss, changes in land use patterns, disease, climate change, synergistic effects. Amphibian have provided better ontogenetic diversity, tissue regeneration, life history reproductive modes, antipredator strategies, resilience adaptive responses. They also serve as essential models studying broad traits, such evolutionary genome expansions contractions, they exhibit widest range sizes among all taxa possess multiple mechanisms genetic sex determination. Despite these features, sequencing amphibians has significantly lagged behind vertebrates, primarily challenges assembling large, repeat-rich genomes relative lack societal support. emergence long-read technologies, combined advanced molecular computational techniques improve scaffolding reduce workloads, now making it possible address some challenges. To promote accelerate production research international coordination collaboration, we launched Genomics Consortium (AGC, https://mvs.unimelb.edu.au/amphibian-genomics-consortium) early 2023. This burgeoning community already 282 members from 41 countries. AGC aims leverage capabilities its advance bridge implementation gap biologists, bioinformaticians, conservation practitioners. Here evaluate state field genomics, highlight previous studies, present overcome, call on communities unite part enable "leap" next level.
Язык: Английский
Процитировано
3
Developmental & Comparative Immunology, Год журнала: 2024, Номер 160, С. 105237 - 105237
Опубликована: Авг. 3, 2024
Язык: Английский
Процитировано
1
BMC Genomics, Год журнала: 2024, Номер 25(1)
Опубликована: Ноя. 1, 2024
Amphibians represent a diverse group of tetrapods, marked by deep divergence times between their three systematic orders and families. Studying amphibian biology through the genomics lens increases our understanding features this animal class that other terrestrial vertebrates. The need for genomic resources is more urgent than ever due to increasing threats group. are one most imperiled taxonomic groups, with approximately 41% species threatened extinction habitat loss, changes in land use patterns, disease, climate change, synergistic effects. Amphibian have provided better ontogenetic diversity, tissue regeneration, life history reproductive modes, anti-predator strategies, resilience adaptive responses. They also serve as essential models studying broad traits, such evolutionary genome expansions contractions, they exhibit widest range sizes among all taxa possess multiple mechanisms genetic sex determination. Despite these features, sequencing amphibians has significantly lagged behind vertebrates, primarily challenges assembling large, repeat-rich genomes relative lack societal support. emergence long-read technologies, combined advanced molecular computational techniques improve scaffolding reduce workloads, now making it possible address some challenges. To promote accelerate production research international coordination collaboration, we launched Genomics Consortium (AGC, https://mvs.unimelb.edu.au/amphibian-genomics-consortium ) early 2023. This burgeoning community already 282 members from 41 countries. AGC aims leverage capabilities its advance bridge implementation gap biologists, bioinformaticians, conservation practitioners. Here evaluate state field genomics, highlight previous studies, present overcome, call on communities unite part enable "leap" next level.
Язык: Английский
Процитировано
1
ImmunoHorizons, Год журнала: 2023, Номер 7(10), С. 696 - 707
Опубликована: Окт. 1, 2023
Abstract The amphibian Xenopus laevis tadpole provides a unique comparative experimental organism for investigating the roles of innate-like T (iT) cells in tolerogenic immunity during early development. Unlike mammals and adult frogs, where conventional are dominant, tadpoles rely mostly on several prominent distinct subsets iT interacting with cognate nonpolymorphic MHC class I–like molecules. In present study, to investigate whole cell responsiveness ontogenesis X. laevis, we determined frogs capacity splenic proliferate vivo upon infection two different pathogens, ranavirus FV3 Mycobacterium marinum, as well vitro PHA stimulation using thymidine analogous 5-ethynyl-2′-deoxyuridine flow cytometry. We also analyzed by RT-quantitative PCR stimulation. showed limited proliferate, whereas proliferation rate was higher than cells. Gene markers activation immediate-early genes induced TCR were upregulated similar kinetics splenocytes. However, signature included lower amplitude signaling, which is hallmark mammalian memory-like or “preset” This study suggests that reminiscent neonatal cells, functionally from their counterpart.
Язык: Английский
Процитировано
2
Gene Reports, Год журнала: 2024, Номер 35, С. 101915 - 101915
Опубликована: Апрель 5, 2024
Язык: Английский
Процитировано
0
Elsevier eBooks, Год журнала: 2024, Номер unknown
Опубликована: Янв. 1, 2024
Язык: Английский
Процитировано
0
Developmental & Comparative Immunology, Год журнала: 2024, Номер 160, С. 105238 - 105238
Опубликована: Авг. 6, 2024
Язык: Английский
Процитировано
0
Опубликована: Окт. 11, 2024
Abstract Ranaviruses (RV, family Iridoviridae ) infect fish, amphibians, and reptiles, raising considerable ecological commercial concerns due to the escalating infection prevalence resulting die-offs of wild aquacultural species. Notably, ranaviruses exhibit uncanny capacities cross host species barriers, likely owing their potent immune evasion mechanisms. In turn, infected by these pathogens possess systems that are less well understood than those mammals often encode unique antiviral genes or multiple orthologs single hallmark mammalian factors. Thus, garnering insight into ranavirus strategies is largely contingent on gaining greater understanding barriers faced emerging infectious agents. Accordingly, here we coalesce update current state distinct facets ectothermic vertebrate responses ranaviral infections underline most perspectives which circumvent defenses.
Язык: Английский
Процитировано
0