Altered human oligodendrocyte heterogeneity in multiple sclerosis

Nature, Год журнала: 2019, Номер 566(7745), С. 543 - 547

Опубликована: Янв. 23, 2019

Язык: Английский

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Disease-specific oligodendrocyte lineage cells arise in multiple sclerosis

Nature Medicine, Год журнала: 2018, Номер 24(12), С. 1837 - 1844

Опубликована: Окт. 31, 2018

Язык: Английский

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A high-resolution transcriptomic and spatial atlas of cell types in the whole mouse brain

Nature, Год журнала: 2023, Номер 624(7991), С. 317 - 332

Опубликована: Дек. 13, 2023

The mammalian brain consists of millions to billions cells that are organized into many cell types with specific spatial distribution patterns and structural functional properties1-3. Here we report a comprehensive high-resolution transcriptomic cell-type atlas for the whole adult mouse brain. was created by combining single-cell RNA-sequencing (scRNA-seq) dataset around 7 million profiled (approximately 4.0 passing quality control), approximately 4.3 using multiplexed error-robust fluorescence in situ hybridization (MERFISH). is hierarchically 4 nested levels classification: 34 classes, 338 subclasses, 1,201 supertypes 5,322 clusters. We present an online platform, Allen Brain Cell Atlas, visualize whole-brain along MERFISH datasets. systematically analysed neuronal non-neuronal across identified high degree correspondence between identity specificity each type. results reveal unique features organization different regions-in particular, dichotomy dorsal ventral parts part contains relatively fewer yet highly divergent types, whereas more numerous closely related other. Our study also uncovered extraordinary diversity heterogeneity neurotransmitter neuropeptide expression co-expression types. Finally, found transcription factors major determinants classification combinatorial factor code defines all establishes benchmark reference foundational resource integrative investigations cellular circuit function, development evolution

Язык: Английский

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Single-cell CUT&Tag profiles histone modifications and transcription factors in complex tissues

Nature Biotechnology, Год журнала: 2021, Номер 39(7), С. 825 - 835

Опубликована: Апрель 12, 2021

Язык: Английский

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Oligodendrocyte Progenitor Cells Become Regionally Diverse and Heterogeneous with Age

Neuron, Год журнала: 2019, Номер 101(3), С. 459 - 471.e5

Опубликована: Янв. 15, 2019

Oligodendrocyte progenitor cells (OPCs), which differentiate into myelinating oligodendrocytes during CNS development, are the main proliferative in adult brain. OPCs conventionally considered a homogeneous population, particularly with respect to their electrophysiological properties, but this has been debated. We show, by using single-cell recordings, that start out as population become functionally heterogeneous, varying both within and between brain regions age. These changes correlate differentiation potential of OPCs; thus, they may underlie differentiational differences and, likewise, failure

Язык: Английский

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Spatial epigenome–transcriptome co-profiling of mammalian tissues

Nature, Год журнала: 2023, Номер 616(7955), С. 113 - 122

Опубликована: Март 15, 2023

Abstract Emerging spatial technologies, including transcriptomics and epigenomics, are becoming powerful tools for profiling of cellular states in the tissue context 1–5 . However, current methods capture only one layer omics information at a time, precluding possibility examining mechanistic relationship across central dogma molecular biology. Here, we present two technologies spatially resolved, genome-wide, joint epigenome transcriptome by cosequencing chromatin accessibility gene expression, or histone modifications (H3K27me3, H3K27ac H3K4me3) expression on same section near-single-cell resolution. These were applied to embryonic juvenile mouse brain, as well adult human map how epigenetic mechanisms control transcriptional phenotype cell dynamics tissue. Although highly concordant features identified either also observed distinct patterns, suggesting their differential roles defining states. Linking pixel allows uncovering new insights priming, differentiation regulation within architecture. great interest life science biomedical research.

Язык: Английский

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Functionally distinct subgroups of oligodendrocyte precursor cells integrate neural activity and execute myelin formation

Nature Neuroscience, Год журнала: 2020, Номер 23(3), С. 363 - 374

Опубликована: Фев. 17, 2020

Язык: Английский

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Single-Cell Transcriptomics Uncovers Glial Progenitor Diversity and Cell Fate Determinants during Development and Gliomagenesis

Cell stem cell, Год журнала: 2019, Номер 24(5), С. 707 - 723.e8

Опубликована: Апрель 11, 2019

Язык: Английский

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Origins and Proliferative States of Human Oligodendrocyte Precursor Cells

Cell, Год журнала: 2020, Номер 182(3), С. 594 - 608.e11

Опубликована: Июль 16, 2020

Язык: Английский

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Oligodendrocyte lineage cells and depression

Molecular Psychiatry, Год журнала: 2020, Номер 26(1), С. 103 - 117

Опубликована: Ноя. 3, 2020

Abstract Depression is a common mental illness, affecting more than 300 million people worldwide. Decades of investigation have yielded symptomatic therapies for this disabling condition but not led to consensus about its pathogenesis. There are data support several different theories causation, including the monoamine hypothesis, hypothalamic–pituitary–adrenal axis changes, inflammation and immune system alterations, abnormalities neurogenesis conducive environmental milieu. Research in these areas others has greatly advanced current understanding depression; however, there other, less widely known Oligodendrocyte lineage cells, oligodendrocyte progenitor cells mature oligodendrocytes, numerous important functions, which include forming myelin sheaths that enwrap central nervous axons, supporting axons metabolically, mediating certain forms neuroplasticity. These specialized glial been implicated psychiatric disorders such as depression. In review, we summarize recent findings shed light on how might participate pathogenesis depression, discuss new approaches targeting novel strategy treat

Язык: Английский

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