Zones of cellular damage around pulsed-laser wounds

PLoS ONE, Год журнала: 2021, Номер 16(9), С. e0253032 - e0253032

Опубликована: Сен. 27, 2021

After a tissue is wounded, cells surrounding the wound adopt distinct wound-healing behaviors to repair tissue. Considerable effort has been spent on understanding signaling pathways that regulate immune and tissue-resident as they respond wounds, but these signals must ultimately originate from physical damage inflicted by wound. Tissue wounds comprise several types of cellular damage, recent work indicates different initiate signaling. Hence understand signaling, it important identify localize wound-induced damage. Laser ablation widely used researchers create reproducible, aseptic in can be live-imaged. Because laser wounding involves combination photochemical, photothermal photomechanical mechanisms, each with spatial dependencies, around pulsed-laser will experience gradient Here we exploit this map Using genetically-encoded fluorescent proteins, monitor damaged sub-cellular components epithelial living Drosophila pupae seconds minutes following wounding. We hypothesized regions would predictably arrayed varying sizes, subsequent analysis found all radii are linearly related over 3-fold range size. Thus, estimated after measuring any one. This report identifies within wounded animal. By quantitatively mapping size placement set foundation for tracing back initiates it.

Язык: Английский

---

Wound Healing Insights from Flies and Fish

Cold Spring Harbor Perspectives in Biology, Год журнала: 2022, Номер unknown, С. a041217 - a041217

Опубликована: Июль 11, 2022

Anne George and Paul Martin School of Biochemistry, Faculty Life Sciences, University Bristol, Bristol BS8 1TD, United Kingdom Correspondence: paul.martin{at}bristol.ac.uk; anne.george{at}bristol.ac.uk

Язык: Английский

---

What good is maths in studies of wound healing?

iScience, Год журнала: 2022, Номер 25(8), С. 104778 - 104778

Опубликована: Июль 19, 2022

Wound healing is an aspect of normal physiology that we all take for granted until it goes wrong, such as, example, the scarring results from a severe burn, or those patients who suffer debilitating chronic wounds fail to heal. Ever since wound repair research began as discipline, clinicians and basic scientists have collaborated try understand cell molecular mechanisms underpin healthy in hope this will reveal clues therapeutic treatment pathological healing. In recent decades mathematicians physicists begun join with important challenge. Here describe examples how mathematical modeling married biological experimentation has provided insights biology alone could not fathom. To date, these studies largely focused on re-epithelialization inflammation, but also discuss other components might be ripe similar interdisciplinary approaches.

Язык: Английский

---

Contractile and expansive actin networks in Drosophila: Developmental cell biology controlled by network polarization and higher-order interactions

Current topics in developmental biology/Current Topics in Developmental Biology, Год журнала: 2023, Номер unknown, С. 99 - 129

Опубликована: Янв. 1, 2023

Язык: Английский

---

Understanding the diversity and dynamics of in vivo efferocytosis: Insights from the fly embryo

Immunological Reviews, Год журнала: 2023, Номер 319(1), С. 27 - 44

Опубликована: Авг. 17, 2023

Summary The clearance of dead and dying cells, termed efferocytosis, is a rapid efficient process one that critical for organismal health. extraordinary speed efficiency with which cells are detected engulfed by immune within tissues presents challenge to researchers who wish unravel this fascinating process, since these fleeting moments uptake almost impossible catch in vivo. In recent years, the fruit fly ( Drosophila melanogaster) embryo has emerged as powerful model circumvent problem. With its abundance specialist phagocytes relative ease live imaging, humble provides unique opportunity study moment cell engulfment real‐time living animal. review, we explore advances have come from studies fly, how imaging genetics revealed previously unappreciated level diversity efferocytic program. A variety strategies across phagocytic population ensure corpses wherever death encountered varied complex setting multicellular organism.

Язык: Английский

---

Sensing of dietary amino acids and regulation of calcium dynamics in adipose tissues through Adipokinetic hormone inDrosophila

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Март 8, 2024

Abstract Nutrient sensing and the subsequent metabolic responses are fundamental functions of animals, closely linked to diseases such as type 2 diabetes various obesity-related diseases. Drosophila melanogaster has emerged an excellent model for investigating metabolism its associated disorders. In this study, we used live-cell imaging demonstrate that fly functional homolog mammalian glucagon, Adipokinetic hormone (AKH), secreted from AKH hormone-producing cells (APCs) in corpora cardiaca, stimulates intracellular Ca 2+ waves larval fat body/adipose tissue promote lipid metabolism. Further, show specific dietary amino acids activate APCs, leading increased secretion. Finally, a comparison dynamics adult bodies revealed different mechanisms regulation, highlighting interplay pulses secretion, extracellular diffusion hormone, intercellular communication through gap junctions. Our study underscores suitability powerful exploring real-time nutrient inter-organ dynamics.

Язык: Английский

---

Wound-Induced Syncytia Outpace Mononucleate Neighbors during Drosophila Wound Repair

Опубликована: Март 27, 2024

All organisms have evolved to respond injury. Cell behaviors like proliferation, migration, and invasion replace missing cells close wounds. However, the role of other wound-induced cell is not understood, including formation syncytia (multinucleated cells). Wound-induced epithelial were first reported around puncture wounds in post-mitotic Drosophila epidermal tissues, but more recently been mitotically competent tissues such as pupal epidermis zebrafish epicardium. The presence active suggests that offer adaptive benefits, it unknown what those benefits are. Here, we use vivo live imaging analyze pupae. We find almost half near a wound fuse form large syncytia. These several routes speed repair: they outpace diploid complete closure; reduce intercalation during pool resources their component concentrate them toward wound. In addition healing, these properties are likely contribute roles development pathology.

Язык: Английский

---

Heterotrimeric G proteins regulate planarian regeneration and behavior

Genetics, Год журнала: 2023, Номер 223(4)

Опубликована: Фев. 10, 2023

Abstract G protein-coupled receptors play broad roles in development and stem cell biology, but few for receptor signaling complex tissue regeneration have been uncovered. Planarian flatworms robustly regenerate all tissues provide a model with which to explore potential functions somatic pluripotent biology. As first step toward exploring function planarians, we investigated downstream signal transducers that work receptors, called heterotrimeric proteins. Here, characterized the complete protein complement Schmidtea mediterranea time found 7 subunits promote regeneration. We further 2 critical regeneration, Gαq1 Gβ1-4a, finding they late phase of anterior polarity reestablishment, likely through pole-produced Follistatin. Incidentally, also 5 modulate planarian behavior. identified putative serotonin receptor, gcr052, propose works Gαs2 Gβx2 locomotion, demonstrating utility our strategy identifying relevant receptors. Our provides foundational insight into proteins biology serves as useful springboard broadening understanding adult

Язык: Английский

---

The force of cell-cell adhesion in determining the outcome in a nonlocal advection diffusion model of wound healing

Mathematical Biosciences & Engineering, Год журнала: 2022, Номер 19(9), С. 8689 - 8704

Опубликована: Янв. 1, 2022

<abstract><p>A model of wound healing is presented to investigate the connection force cell-cell adhesion sensing radius cells in their spatial environment. The consists a partial differential equation with nonlocal advection and diffusion terms, describing movement applied biological experiments understand incomplete closure. analysis demonstrates that for each value parameter, there critical above which complete does not occur.</p></abstract>

Язык: Английский

---

Parallel repair mechanisms in plants and animals

Disease Models & Mechanisms, Год журнала: 2023, Номер 16(1)

Опубликована: Янв. 1, 2023

ABSTRACT All organisms have acquired mechanisms for repairing themselves after accidents or lucky escape from predators, but how analogous are these across phyla? Plants and animals distant relatives in the tree of life, both need to be able efficiently repair themselves, they will perish. Both an outer epidermal barrier layer a circulatory system that must protect infection. However, plant cells immotile with rigid cell walls, so cannot raise animal-like immune response move away insult, as can. Here, we discuss parallel strategies signalling pathways used by plants heal their tissues, well key differences. A more comprehensive understanding parallels differences could highlight potential avenues enhance healing patients’ wounds clinic and, reciprocal way, developing novel alternatives agricultural pesticides.

Язык: Английский

---