KatB, a bacterial extracellular vesicles (EVs)‐secreted catalase, detoxifies reactive oxygen species (ROS) and promotes pathogen proliferation in plants

Journal of Integrative Plant Biology, Год журнала: 2025, Номер unknown

Опубликована: Март 27, 2025

ABSTRACT Gram‐negative bacteria are known to release extracellular vesicles (EVs) into their surrounding environment. However, the biological functions of proteins contained within these remain largely unknown. Here, we used tandem mass tag (TMT) proteomic analysis characterize protein cargoes EVs phytopathogen Pseudomonas syringae pv. tomato DC3000 ( Pto DC3000). Our investigation revealed that one catalase, KatB, is enriched in bacterial EVs. This enzyme confers with capacity detoxify both exogenous and plant‐produced H 2 O , thereby contributing pathogen's proliferation plants. Interestingly, reactive oxygen species (ROS) stress stimulates EV secretion enhances package KatB vesicles. regulatory process depends on a periplasmic ankyrin‐like protein, AnkB. Both AnkB encoded small operon, mutant strains exhibit impaired growth plant hosts. Furthermore, treatment pelleted from culture supernatants activates immune responses plants, absence further this protective activity. Collectively, our findings indicate secreted via interact reduce host's oxidative environment, promoting

Язык: Английский

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Extracellular vesicle-mediated plant miRNA trafficking regulates viral infection in insect vector

Cell Reports, Год журнала: 2025, Номер 44(5), С. 115635 - 115635

Опубликована: Апрель 27, 2025

Язык: Английский

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Plant‐derived vesicle‐like nanoparticles in food crops: emerging insights into nutritional biofortification and biomedical applications

Plant Biotechnology Journal, Год журнала: 2025, Номер unknown

Опубликована: Май 26, 2025

Summary Food crops, as the cornerstone of human nutrition, not only provide essential macronutrients but also encompass a rich spectrum bioactive compounds that promote health. Of particular significance are underutilized minor which contain unique beneficial components with proven nutraceutical properties. These valuable can be effectively concentrated into plant‐derived vesicle‐like nanoparticles (PDVLNs), play crucial roles in maintaining health and reducing disease risks. Furthermore, owing to their inherent stability low immunogenicity, PDVLNs serve an innovative natural eco‐friendly delivery platform applications spanning agricultural production, particularly crop nutrient fortification biomedicine. Consequently, derived from food crops have emerged pivotal research focus, garnering substantial scientific attention. However, comprehensive review crop‐derived potential remains lacking. This presents in‐depth analysis nutritional composition distribution patterns two major groups– cereals legumes– while systematically examining current advances future directions enhancement. Additionally, we elucidate fundamental characteristics functions PDVLNs, explore recent breakthroughs crops. Finally, offer novel perspectives for understanding special emphasis on value compounds.

Язык: Английский

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Arabidopsis Produces Distinct Subpopulations of Extracellular Vesicles That Respond Differentially to Biotic Stress, Altering Growth and Infectivity of a Fungal Pathogen

Journal of Extracellular Vesicles, Год журнала: 2025, Номер 14(5)

Опубликована: Май 1, 2025

ABSTRACT Extracellular vesicles (EVs) secreted by mammalian cells are highly heterogeneous in content and function. Whether this is also true for EVs plant not yet known. To address this, we used high‐resolution density gradient ultracentrifugation total internal fluorescence microscopy (TIRF‐M) to purify distinguish distinct subpopulations of Arabidopsis EVs. The EV marker protein TETRASPANIN 8 (TET8) was detected specifically medium‐density TET8 PENETRATION 1 (PEN1) were confirmed be mostly separate populations using TIRF‐M, while PEN1 co‐secreted with 3 (PEN3) much more often. Secretion marked TET8, RPM1‐INTERACTING PROTEIN 4 (RIN4) into the apoplast onto leaf surface induced phytohormones, changes temperature infection fungal pathogens. Treatment seedlings delayed progression altering germ tube development morphology. Significantly, extracellular RNAs, including miRNAs siRNAs, did co‐fractionate TET8‐labeled EVs, instead, co‐fractionated extravesicular ARGONAUTE proteins high‐density fractions. Together, these data indicate that contribute immunity but unlikely mediate cross‐kingdom RNA interference.

Язык: Английский

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Extracellular vesicle-mediated RNA warfare: A cross-kingdom battleground

Current Opinion in Plant Biology, Год журнала: 2025, Номер 86, С. 102741 - 102741

Опубликована: Июнь 2, 2025

Язык: Английский

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