Microdroplet engineered skeletal muscle organoids from primary tissue recapitulate parental physiology with high reproducibility

Research, Год журнала: 2025, Номер 8

Опубликована: Янв. 1, 2025

Achieving high maturity and functionality in vitro skeletal muscle models is essential for advancing our understanding of biology, disease mechanisms, drug discovery. However, current struggle to fully recapitulate key features such as sarcomere structure, fiber composition, contractile function while also ensuring consistency rapid production. Adult stem cells residing tissue are known their powerful regenerative potential, yet tissue-derived organoids have not been established. In this study, we introduce droplet-engineered derived from primary using cascade-tubing microfluidics. These (DEOs) exhibit maturity, including well-developed striated sarcomeres, spontaneous stimulated contractions, recapitulation parental types. Notably, DEOs produced just 8 d without the need cell culture—substantially accelerating 50- 60-d process required by classical organoid models. Additionally, microfluidics platform enables high-throughput production hundreds uniform DEO replicates a small sample, providing scalable reproducible solution research screening.

Язык: Английский

Recent advancements and challenges in 3D bioprinting for cancer applications

Bioprinting, Год журнала: 2024, Номер 43, С. e00357 - e00357

Опубликована: Сен. 13, 2024

Язык: Английский