Five doses of the mRNA vaccination potentially suppress ancestral-strain stimulated SARS-CoV2-specific cellular immunity: a cohort study from the Fukushima vaccination community survey, Japan
Frontiers in Immunology,
Год журнала:
2023,
Номер
14
Опубликована: Авг. 16, 2023
The
bivalent
mRNA
vaccine
is
recommended
to
address
coronavirus
disease
variants,
with
additional
doses
suggested
for
high-risk
groups.
However,
the
effectiveness,
optimal
frequency,
and
number
of
remain
uncertain.
In
this
study,
we
examined
long-term
cellular
humoral
immune
responses
following
fifth
administration
severe
acute
respiratory
syndrome
2
(SARS-CoV-2)
in
patients
undergoing
hemodialysis.
To
our
knowledge,
first
study
monitor
data
on
immunity
dynamics
populations
after
five
vaccination,
including
vaccine.
Whereas
most
maintained
throughout
observation
period,
observed
reduced
reactivity
as
measured
by
ancestral-strain-stimulated
ELISpot
assay
a
subset
patients.
Half
individuals
(50%;
14/28)
three
months
dose,
despite
acquiring
immunity.
absence
relationship
between
positive
controls
T-Spot
suggests
that
these
alterations
were
specific
SARS-CoV-2.
multivariable
analysis,
participants
aged
≥70
years
showed
marginally
significant
lower
likelihood
having
reactive
results.
Notably,
among
14
who
received
heterologous
vaccines,
13
successfully
acquired
immunity,
supporting
effectiveness
strategy.
These
findings
provide
valuable
insights
future
vaccination
strategies
vulnerable
populations.
further
research
needed
evaluate
involvement
tolerance
exhaustion
through
repeated
optimize
immunization
strategies.
Язык: Английский
Natural and hybrid immunity after SARS-CoV-2 infection in children and adolescents
Infection,
Год журнала:
2024,
Номер
52(4), С. 1449 - 1458
Опубликована: Март 18, 2024
Abstract
Purpose
In
contrast
to
adults,
immune
protection
against
SARS-CoV-2
in
children
and
adolescents
with
natural
or
hybrid
immunity
is
still
poorly
understood.
The
aim
of
this
study
was
analyze
different
compartments
age
groups
whether
humoral
reactions
correlate
a
cellular
response.
Methods
72
preceding
infection
were
recruited.
37
vaccinated
an
RNA
vaccine
(BNT162b2).
Humoral
analyzed
3–26
months
(median
10
months)
after
by
measuring
Spike
protein
(S),
nucleocapsid
(NCP),
neutralizing
antibodies
(nAB).
Cellular
using
SARS-CoV-2-specific
interferon-γ
release
assay
(IGRA).
Results
All
had
S
antibodies;
titers
higher
those
(14,900
BAU/ml
vs.
2118
BAU/ml).
NCP
detectable
>
90%.
Neutralizing
(nAB)
more
frequently
detected
(90%)
(1914
RLU)
than
(62.5%,
476
RLU).
Children
less
likely
have
reactive
IGRAs
(43.8%)
(85%).
amount
released
T
cells
comparable
immunity.
Conclusion
are
the
most
reliable
markers
monitor
reaction
SARS-CoV-2.
High
antibody
spike
nAB
correlated
immunity,
phenomenon
found
only
Hybrid
associated
markedly
probability
response
Язык: Английский
Cellular Immunity of SARS-CoV-2 in the Borriana COVID-19 Cohort: A Nested Case–Control Study
Epidemiologia,
Год журнала:
2024,
Номер
5(2), С. 167 - 186
Опубликована: Апрель 10, 2024
Our
goal
was
to
determine
the
cellular
immune
response
(CIR)
in
a
sample
of
Borriana
COVID-19
cohort
(Spain)
identify
associated
factors
and
their
relationship
with
infection,
reinfection
sequelae.
We
conducted
nested
case-control
study
using
randomly
selected
225
individuals
aged
18
older,
including
36
naïve
SARS-CoV-2
infection
189
infected
patients.
employed
flow-cytometry-based
immunoassays
for
intracellular
cytokine
staining,
Wuhan
BA.2
antigens,
chemiluminescence
microparticle
immunoassay
detect
antibodies.
Logistic
regression
models
were
applied.
A
total
215
(95.6%)
participants
exhibited
T-cell
(TCR)
at
least
one
antigen.
Positive
responses
CD4+
CD8+
T
cells
89.8%
85.3%,
respectively.
No
difference
CIR
found
between
Patients
who
experienced
sequelae
higher
than
those
without.
positive
correlation
observed
TCR
anti-spike
IgG
levels.
Factors
positively
included
blood
group
A,
number
vaccine
doses
received,
anti-N
IgM;
inversely
related
time
elapsed
since
last
dose
or
B.
These
findings
contribute
valuable
insights
into
nuanced
landscape
shaped
by
vaccination.
Язык: Английский
Comparative Assessment of the Kinetics of Cellular and Humoral Immune Responses to COVID-19 Vaccination in Cancer Patients
Viruses,
Год журнала:
2023,
Номер
15(7), С. 1439 - 1439
Опубликована: Июнь 26, 2023
Objective:
The
kinetics
of
immune
responses
to
various
SARS-CoV-2
vaccines
in
cancer
patients
were
investigated.
Methods:
In
total,
57
who
received
BNT162b2-RNA
or
BBIBP-CorV
enrolled.
Cellular
and
humoral
immunity
assessed
at
three-time
points,
before
the
first
vaccine
dose
14–21
days
after
second
doses.
Chemiluminescent
microparticle
immunoassay
was
used
evaluate
anti-spike
IgG
response,
QuantiFERON®
kit
T-cell
response.
Results:
Data
showed
that
patients’
CD4+
CD8+
T
cell-median
IFN-γ
secretion
antigens
increased
doses
(p
=
0.027
p
0.042).
BNT162b2
vaccinees
had
significantly
higher
levels
cell
epitopes
than
0.028).
There
a
positive
correlation
between
antibody
titer
response
regardless
type
<
0.05).
Conclusions:
This
study
is
one
investigate
cellular
immunization
on
active
therapy
each
dose.
COVID-19
immunizations
helped
develop
an
effective
Understanding
undergoing
treatment
necessary
improve
avoid
future
SARS
pandemics.
Язык: Английский
Comparative Study of T-Cell Repertoires after COVID-19 Immunization with Homologous or Heterologous Vaccine Booster
Pathogens,
Год журнала:
2024,
Номер
13(4), С. 284 - 284
Опубликована: Март 27, 2024
Sequencing
of
the
T-cell
repertoire
is
an
innovative
method
to
assess
cellular
responses
after
immunization.
The
purpose
this
study
was
compare
repertoires
COVID-19
immunization
with
homologous
(HOB)
and
heterologous
(HEB)
boosting.
included
20
participants
a
median
age
27.5
(IQR:23)
years,
who
were
vaccinated
one
dose
Ad26.COV2.S
vaccine
boosted
either
(n
=
10)
or
BNT162b2
vaccine.
Analysis
receptor
beta
locus
(TCRβ)
sequencing
month
booster
identified
that
HEB
compared
HOB
group
exhibited
higher
number
both
total
COVID-19-related
functional
rearrangements
[mean
productive
(TPRs):
63151.8
(SD
±
18441.5)
vs.
34915.4
11121.6),
p
0.001
COVID-19–TPRs:
522.5
178.0)
298.3
101.1),
0.003].
A
comparison
between
groups
detected
no
statistically
significant
differences
regarding
Simpson
clonality
[0.021
(IQR:0.014)
0.019
(IQR:0.007)],
richness
[8734.5
(IQR:973.3)
8724
(IQR:383.7)]
fraction
[0.19
(IQR:0.08)
0.18
(IQR:0.08)].
also
substantially
elevated
humoral
immune
response
[median
antibody
titer
(IQR):
10115.0
U/mL
(6993.0)
1781.0
(1314.0),
0.001].
indicated
had
increased
SARS-CoV-2-related
rearrangements,
which
in
accordance
possibly
conferring
longer
protection.
Data
from
present
indicate
administration
different
vaccines
as
may
provide
better
Язык: Английский
Study of the cellular and humoral immune responses to SARS-CoV-2 vaccination
Heliyon,
Год журнала:
2024,
Номер
10(7), С. e29116 - e29116
Опубликована: Апрель 1, 2024
Our
understanding
of
cellular
immunity
in
response
to
COVID-19
infection
or
vaccination
is
limited
because
less
commonly
used
techniques.
We
investigated
both
the
and
humoral
immune
responses
before
after
administration
a
third
dose
SARS-CoV-2
vaccine
among
group
healthcare
workers.
Cellular
was
evaluated
using
VIDAS
interferon-gamma
(IFNγ)
RUO
test,
which
enables
automated
measurement
IFNγ
levels
stimulating
peripheral
blood
lymphocytes.
Booster
doses
significantly
enhanced
immunity.
Concerning
response,
booster
increased
percentage
positive
release
assay
(IGRA)
results
but
no
difference
found.
The
not
associated
with
protection
against
infection.
Interestingly,
vaccinated
infected
workers
exhibited
highest
anti-spike
neutralizing
antibodies.
In
conclusion,
IGRA
simple
method
for
measuring
vaccination.
However,
its
usefulness
as
complement
study
yet
be
demonstrated
future
research.
Язык: Английский
QuantiFERON SARS-CoV-2 assay for the evaluation of cellular immunity after immunization with mRNA SARS-CoV-2 vaccines: A Systematic Review and Meta-Analysis
Research Square (Research Square),
Год журнала:
2024,
Номер
unknown
Опубликована: Апрель 30, 2024
Abstract
A
systematic
review
and
meta-analysis
was
performed
to
evaluate
the
virus-specific
T-cell
response
after
COVID-19
mRNA
vaccination,
using
QuantiFERON-SARS-CoV-2
Interferon-γ
Release
Assay.
search
conducted
(June
8,
2023)
in
PUBMED,
SCOPUS
medRxiv
databases,
identify
studies
reporting
[Starter
(two
Antigen
tubes)
or
Starter+Extended
Pack
(three
tubes),
cut-off³0.15IU/mL]
Positivity
Rate
(PR)
immunocompetent
adults,
following
administration
of
two
three
vaccine
doses.
Study
quality
evaluated
with
Critical
Appraisal
Skills
Program
Tool.
a
Random-Effects
model.
Heterogeneity
publication
bias
were
assessed.
Eleven
eligible
(with
5-75
vaccinated
participants)
identified.
For
COVID-19-naive
participants,
£3
months
second
dose,
pooled
PR
(Random-Effects
model)
81
[95%
Confidence
Interval
(95%CI):71-92]%.
Comparing
Starter
vs.
Pack,
significant
difference
PRs
detected
(79.3%
92.2%,
p-value=0.039).
At
3-6
>6
dose
at
³3
third
59(95%CI:45-72)%,
79(95%CI:66-92)%
66(95%CI:50-82)%,
respectively.
convalescent
³6
81(95%CI:67-92)%.
In
conclusion,
vaccine
,
QuantiFERON-SARS-CoV-2
positive
responses
certain
percentage
vaccinees.
This
detection
higher
when
used.
Possible
explanations
for
assay’s
negative
results
subset
participants
could
be:
waning
immunity,
reduced
sensitivity
compared
other
assays
lack
induction
some
vaccinees.
(PROSPERO
Registration
Number:CRD42023431315)
Язык: Английский
Comparison οf Immune Responses Through Multiparametric T-Cell Cytokine Expression Profile Between Children with Convalescent COVID-19 or Multisystem Inflammatory Syndrome
Children,
Год журнала:
2024,
Номер
11(11), С. 1278 - 1278
Опубликована: Окт. 23, 2024
The
immunological
pathways
that
cause
Multisystem
Inflammatory
Syndrome
after
SARS-CoV-2
infection
in
children
(MIS-C)
remain
under
investigation.
Язык: Английский
QuantiFERON SARS-CoV-2 assay for the evaluation of cellular immunity after immunization with mRNA SARS-CoV-2 vaccines: a systematic review and meta-analysis
Immunologic Research,
Год журнала:
2024,
Номер
73(1)
Опубликована: Дек. 27, 2024
Язык: Английский
Natural and hybrid immunity after SARS-CoV-2 infection in children and adolescents
Research Square (Research Square),
Год журнала:
2023,
Номер
unknown
Опубликована: Окт. 12, 2023
Abstract
Purpose
The
immune
protection
in
children
and
adolescents
with
natural
or
hybrid
immunity
(vaccination
&
infection)
against
SARS-CoV-2
remains
an
understudied
field.
Aim
of
this
study
was
to
analyze
different
compartments
age
groups
whether
humoral
reactions
correlate
a
cellular
response.
Methods
72
preceding
infection
were
recruited.
37
vaccinated
RNA-vaccine
(BNT162b2).
Humoral
analyzed
3
26
months
(median
10
months)
after
by
measuring
Spike
protein
(S),
nucleocapsid
(NCP)
neutralizing
antibodies
(nAB).
Cellular
using
specific
interferon-γ
release
assay
(IGRA).
Results
All
had
S
antibodies;
titers
higher
those
(14900
BAU/ml
vs.
2118
BAU/ml).
NCP
detectable
>
90%.
Neutralizing
(nAB)
more
frequently
detected
(90%)
(1914
RLU)
than
(62,5%,
476
RLU).
Children
less
likely
have
reactive
IGRAs
(43,8%)
(85%).
amount
released
T
cells
comparable
immunity.
Conclusion
are
the
most
reliable
markers
monitor
reaction
SARS-CoV-2.
High
antibody
nAB
correlated
immunity,
phenomenon
found
only
Hybrid
is
associated
markedly
(S
nAB)
probability
response
Язык: Английский