Mass Spectrometry as a Quantitative Tool for SpCas9 sgRNA Quality Control DOI

Juan Daniel Avila,

Puzhou Wang

Nucleic Acid Therapeutics, Год журнала: 2024, Номер 34(6), С. 299 - 303

Опубликована: Авг. 23, 2024

Mass spectrometry (MS) has long been used for quality control of oligonucleotide therapeutics, including single-guide RNAs (sgRNAs) clustered regularly interspaced short palindromic repeats techniques. However, the application MS is limited to qualitative assays in most cases. Here, we showed that electrospray-ionization quadrupole time-of-flight (ESI-QTOF-MS) can be quantitative chemical species found sgRNA samples. More specifically, using a 100-nt SpCas9 as example, estimated limits quantification length variants range N - 4 + (i.e., 96-104 nucleotides) were equal or lower than 1%. Our study highlighted potential ESI-QTOF its method molecules.

Язык: Английский

Harnessing the power of clustered regularly interspaced short palindromic repeats (CRISPR) based microfluidics for next-generation molecular diagnostics DOI
Rasanpreet Kaur, Saurabh Kumar Gupta, Arjun Chauhan

и другие.

Molecular Biology Reports, Год журнала: 2024, Номер 51(1)

Опубликована: Авг. 8, 2024

Язык: Английский

Процитировано

4

Portable detection of Salmonella in food of animal origin via Cas12a-RAA combined with an LFS/PGM dual-signaling readout biosensor DOI

Yuanshang Wang,

Jianfang Cao,

Pengfei Du

и другие.

Microchimica Acta, Год журнала: 2024, Номер 191(10)

Опубликована: Сен. 28, 2024

Язык: Английский

Процитировано

0

Evaluation of Tissue Specific Mrna Markers for Forensic Body Fluid Identification Based on Qpcr and Rpa-Crispr-Cas12a Techniques DOI
Hui Cai,

Yongsong Zhou,

Xi Yuan

и другие.

Опубликована: Янв. 1, 2024

Язык: Английский

Процитировано

0

Mass Spectrometry as a Quantitative Tool for SpCas9 sgRNA Quality Control DOI

Juan Daniel Avila,

Puzhou Wang

Nucleic Acid Therapeutics, Год журнала: 2024, Номер 34(6), С. 299 - 303

Опубликована: Авг. 23, 2024

Mass spectrometry (MS) has long been used for quality control of oligonucleotide therapeutics, including single-guide RNAs (sgRNAs) clustered regularly interspaced short palindromic repeats techniques. However, the application MS is limited to qualitative assays in most cases. Here, we showed that electrospray-ionization quadrupole time-of-flight (ESI-QTOF-MS) can be quantitative chemical species found sgRNA samples. More specifically, using a 100-nt SpCas9 as example, estimated limits quantification length variants range N - 4 + (i.e., 96-104 nucleotides) were equal or lower than 1%. Our study highlighted potential ESI-QTOF its method molecules.

Язык: Английский

Процитировано

0