Harnessing the power of clustered regularly interspaced short palindromic repeats (CRISPR) based microfluidics for next-generation molecular diagnostics
Molecular Biology Reports,
Год журнала:
2024,
Номер
51(1)
Опубликована: Авг. 8, 2024
Язык: Английский
Portable detection of Salmonella in food of animal origin via Cas12a-RAA combined with an LFS/PGM dual-signaling readout biosensor
Yuanshang Wang,
Jianfang Cao,
Pengfei Du
и другие.
Microchimica Acta,
Год журнала:
2024,
Номер
191(10)
Опубликована: Сен. 28, 2024
Язык: Английский
Evaluation of Tissue Specific Mrna Markers for Forensic Body Fluid Identification Based on Qpcr and Rpa-Crispr-Cas12a Techniques
Опубликована: Янв. 1, 2024
Язык: Английский
Mass Spectrometry as a Quantitative Tool for SpCas9 sgRNA Quality Control
Nucleic Acid Therapeutics,
Год журнала:
2024,
Номер
34(6), С. 299 - 303
Опубликована: Авг. 23, 2024
Mass
spectrometry
(MS)
has
long
been
used
for
quality
control
of
oligonucleotide
therapeutics,
including
single-guide
RNAs
(sgRNAs)
clustered
regularly
interspaced
short
palindromic
repeats
techniques.
However,
the
application
MS
is
limited
to
qualitative
assays
in
most
cases.
Here,
we
showed
that
electrospray-ionization
quadrupole
time-of-flight
(ESI-QTOF-MS)
can
be
quantitative
chemical
species
found
sgRNA
samples.
More
specifically,
using
a
100-nt
SpCas9
as
example,
estimated
limits
quantification
length
variants
range
N
-
4
+
(i.e.,
96-104
nucleotides)
were
equal
or
lower
than
1%.
Our
study
highlighted
potential
ESI-QTOF
its
method
molecules.
Язык: Английский