Evaluation of Perfusion Cell Culture Conditions in a Double-Layered Microphysiological System Using AI-Assisted Morphological Analysis

Micromachines, Год журнала: 2025, Номер 16(3), С. 327 - 327

Опубликована: Март 12, 2025

In recent years, microphysiological systems (MPS) using microfluidic technology as a new in vitro experimental system have shown promise an alternative to animal experiments the development of drugs, especially field drug discovery, and some reports indicated that MPS potential be valuable tool obtain outcomes comparable those experiments. We commercialized Fluid3D-X®, double-layer chip made polyethylene terephthalate (PET), under Japan Agency for Medical Research Development (AMED) research project applied it various organ models. When intestinal epithelial cells, Caco-2, were cultured Fluid3D-X® peristaltic pump, villi-like structures formed microchannels. Still, degree formation differed between upstream downstream sides. To examine consideration points regarding effects nutrient oxygen supply by material medium perfusion rate direction on cells widely used demonstrate usefulness imaging evaluation method artificial intelligence assistive morphological cell morphology channels was quantified evaluated Nikon NIS.ai microscopic observation. Villi-like predominant top channel, independent bottom developed with increased flow rate. Additionally, compared PDMS showed almost uniform sterilization channel. The result indicates environment within microchannels differs because amount nutrients varies depending medium’s chips. As required different types differs, is necessary study optimization culture conditions according characteristics handled. It also demonstrated AI-based image analysis helpful quantification differences microchannel observed microscope.

Язык: Английский

Extracellular Matrix Stiffness: Mechanotransduction and Mechanobiological Response-Driven Strategies for Biomedical Applications Targeting Fibroblast Inflammation

Polymers, Год журнала: 2025, Номер 17(6), С. 822 - 822

Опубликована: Март 20, 2025

The extracellular matrix (ECM) is a dynamic network providing mechanical and biochemical cues that regulate cellular behavior. ECM stiffness critically influences fibroblasts, the primary producers, particularly in inflammation fibrosis. This review explores role of fibroblast-driven tissue remodeling, focusing on physicochemical biological mechanisms involved. Engineered materials, hydrogels, polydimethylsiloxane (PDMS) are highlighted for replicating tissue-specific stiffness, enabling precise control over cell–matrix interactions. surface functionalization substrate including collagen, polydopamine, fibronectin, enhances bioactivity fibroblast adhesion. Key mechanotransduction pathways, such as integrin signaling YAP/TAZ activation, related to regulating behaviors inflammatory responses. fibroblasts driving chronic diseases emphasizes their therapeutic potentials. Advances ECM-modifying strategies, tunable biomaterials hydrogel-based therapies, explored applications engineering, drug delivery, anti-inflammatory treatments, diagnostic tools accurate diagnosis prognosis stiffness-related diseases. integrates mechanobiology with biomedical innovations, comprehensive responses outlining future directions targeted therapies.

Язык: Английский

A Microfluidic Device Integrating a Glucose Sensor and Calibration Function for Cell-Based Assays

Biosensors, Год журнала: 2025, Номер 15(5), С. 307 - 307

Опубликована: Май 11, 2025

Microphysiological systems (MPS) incorporating microfluidic technologies offer improved physiological relevance and real-time analysis for cell-based assays, but often lack non-invasive monitoring capabilities. Addressing this gap, we developed a assay platform integrating an electrochemical biosensor real-time, of kinetic cell status through glucose consumption. The addresses the critical limitations traditional which typically rely on invasive, discontinuous methods. By combining enzyme-modified platinum electrodes within device, our can quantify dynamic changes in concentration resulting from cellular metabolism. We have integrated calibration function that corrects sensor drift, ensuring accurate prolonged short-term measurement stability. In validation experiments, system successfully monitored levels continuously 20 h, demonstrating robust performance reliable predictions. Furthermore, toxicity assays using HepG2 cells exposed to varying concentrations paraquat, detected consumption, effectively quantifying responses. This capability highlights device's potential accurately assessing conditions cells. Overall, significantly enhances by enabling continuous, quantitative, non-destructive analysis, positioning it as valuable tool future drug development biomedical research.

Язык: Английский