Luminescence “on-off-on” sensing of acute myocardial infarction biomarker miRNA-208a based on copper nanoclusters-MoS 2 FRET system DOI Creative Commons
Suzhen Zhang,

Danling Mei,

Hongming Fang

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 12, 2024

Abstract MiRNA-208a is a molecular marker for the early stage of acute myocardial infarction (AMI), with satisfactory specificity and relatively short time window, making it highly suitable diagnosis AMI. In this study, we designed CuNCs-cDNA-MoS2 fluorescent probe established fluorescence resonance energy transfer (FRET) enzyme-free biosensor "on-off-on" detection miRNA-208a. The results showed that limit miRNA-208a was 900 pM, linear range 1 to 10 nM. Interestingly, after addition RNase inhibitor, serum were reduced 5 minutes 380 respectively. It also has wide (1–20 nM), recovery rate 98.13 101.20%. Compared traditional methods, strategy simple, rapid, sensitive, cost-effective. summary, FRET-based sensor provides high-performance method detecting

Язык: Английский

Luminescence “on-off-on” sensing of acute myocardial infarction biomarker miRNA-208a based on copper nanoclusters-MoS 2 FRET system DOI Creative Commons
Suzhen Zhang,

Danling Mei,

Hongming Fang

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 12, 2024

Abstract MiRNA-208a is a molecular marker for the early stage of acute myocardial infarction (AMI), with satisfactory specificity and relatively short time window, making it highly suitable diagnosis AMI. In this study, we designed CuNCs-cDNA-MoS2 fluorescent probe established fluorescence resonance energy transfer (FRET) enzyme-free biosensor "on-off-on" detection miRNA-208a. The results showed that limit miRNA-208a was 900 pM, linear range 1 to 10 nM. Interestingly, after addition RNase inhibitor, serum were reduced 5 minutes 380 respectively. It also has wide (1–20 nM), recovery rate 98.13 101.20%. Compared traditional methods, strategy simple, rapid, sensitive, cost-effective. summary, FRET-based sensor provides high-performance method detecting

Язык: Английский

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