Luminescence “on-off-on” sensing of acute myocardial infarction biomarker miRNA-208a based on copper nanoclusters-MoS 2 FRET system
Abstract
MiRNA-208a
is
a
molecular
marker
for
the
early
stage
of
acute
myocardial
infarction
(AMI),
with
satisfactory
specificity
and
relatively
short
time
window,
making
it
highly
suitable
diagnosis
AMI.
In
this
study,
we
designed
CuNCs-cDNA-MoS2
fluorescent
probe
established
fluorescence
resonance
energy
transfer
(FRET)
enzyme-free
biosensor
"on-off-on"
detection
miRNA-208a.
The
results
showed
that
limit
miRNA-208a
was
900
pM,
linear
range
1
to
10
nM.
Interestingly,
after
addition
RNase
inhibitor,
serum
were
reduced
5
minutes
380
respectively.
It
also
has
wide
(1–20
nM),
recovery
rate
98.13
101.20%.
Compared
traditional
methods,
strategy
simple,
rapid,
sensitive,
cost-effective.
summary,
FRET-based
sensor
provides
high-performance
method
detecting
Research Square (Research Square), Год журнала: 2024, Номер unknown
Опубликована: Ноя. 12, 2024
Язык: Английский