Dysregulation of Porphyromonas gingivalis Agmatine Deiminase Expression in Alzheimer’s Disease DOI

Asma Hamdi,

Sana Baroudi,

Alya Gharbi

и другие.

Current Alzheimer Research, Год журнала: 2024, Номер 21(4), С. 232 - 241

Опубликована: Апрель 1, 2024

Background: Alzheimer’s disease (AD) is the most prevalent neurodegenerative disorder, with a significant burden on global health. AD characterized by progressive cognitive decline and memory loss. Emerging research suggests potential link between periodontitis, specifically presence of oral bacteria such as Porphyromonas gingivalis (P. gingivalis), progression. P. produces an enzyme, Agmatine deiminase (AgD), which converts agmatine to N-carbamoyl putrescine (NCP), serving precursor essential polyamines. Recent studies have confirmed correlation disruptions in polyamine metabolism impairment. Objective: This study aims investigate dysregulation (PgAgD) context AD. Methods: Saliva samples were collected from total 54 individuals, including 27 patients healthy controls. The expression PgAgD gene was analyzed using quantitative Real-- Time PCR. Results: results showed decrease saliva compared downregulation found advanced stages periodontitis. Additionally, observed 30-item Mini-Mental State Examination (MMSE) score. Conclusion: These findings suggest that measuring could be noninvasive tool for monitoring progression aid early diagnosis Further needed validate our explore underlying mechanisms linking expression, pathophysiology.

Язык: Английский

Cutting through the noise: A narrative review of Alzheimer's disease plasma biomarkers for routine clinical use DOI Creative Commons
Michael Schöll, Agathe Vrillon, Takeshi Ikeuchi

и другие.

The Journal of Prevention of Alzheimer s Disease, Год журнала: 2025, Номер unknown, С. 100056 - 100056

Опубликована: Янв. 1, 2025

As novel, anti-amyloid therapies have become more widely available, access to timely and accurate diagnosis has integral ensuring optimal treatment of patients with early-stage Alzheimer's disease (AD). Plasma biomarkers are a promising tool for identifying AD pathology; however, several technical clinical factors need be considered prior their implementation in routine use. Given the rapid pace advancements field wide array available tests, this review aims summarize these considerations, evaluate platforms, discuss steps needed bring plasma biomarker testing clinic. We focus on phosphorylated(p)-tau, specifically p-tau217, as robust candidate across both primary secondary care settings. Despite high performance robustness demonstrated research, like all biomarkers, can affected by analytical pre-analytical variability well patient comorbidities, sex, ethnicity, race. This also discusses advantages two-point cut-off approach mitigating factors, challenges raised resulting intermediate range measurements, where guidance is still unclear. Further validation p-tau217 heterogeneous, real-world cohorts will help increase confidence support establishing standardized approach. poised affordable less invasive alternative PET CSF testing. However, understanding that impact measurement interpretation critical

Язык: Английский

Процитировано

3

Challenges in the practical implementation of blood biomarkers for Alzheimer’s disease DOI Creative Commons
Michael Schöll, Inge M.W. Verberk, Marta del Campo

и другие.

The Lancet Healthy Longevity, Год журнала: 2024, Номер 5(10), С. 100630 - 100630

Опубликована: Окт. 1, 2024

Язык: Английский

Процитировано

17

Advances in blood biomarkers for Alzheimer disease (AD): A review DOI Creative Commons

Araya Dimtsu Assfaw,

Suzanne E. Schindler, John C. Morris

и другие.

The Kaohsiung Journal of Medical Sciences, Год журнала: 2024, Номер 40(8), С. 692 - 698

Опубликована: Июнь 18, 2024

Abstract Alzheimer disease (AD) and Disease Related Dementias (AD/ADRD) are growing public health challenges globally affecting millions of older adults, necessitating concerted efforts to advance our understanding management these conditions. AD is a progressive neurodegenerative disorder characterized pathologically by amyloid plaques tau neurofibrillary tangles that the primary cause dementia in individuals. Early accurate diagnosis crucial for effective intervention treatment but has proven challenging accomplish. Although testing brain pathology with cerebrospinal fluid (CSF) or positron emission tomography (PET) been available over 2 decades, most patients never underwent this because inaccessibility, high out‐of‐pocket costs, perceived risks, lack AD‐specific treatments. However, recent years, rapid progress made developing blood biomarkers AD/ADRD. Consequently, have emerged as promising tools non‐invasive cost‐effective diagnosis, prognosis, monitoring progression. This review presents evolving landscape AD/ADRD explores their potential applications clinical practice early detection, therapeutic interventions. It covers advances biomarkers, including beta (Aβ) peptides, protein, neurofilament light chain (NfL), glial fibrillary acidic protein (GFAP). also discusses diagnostic prognostic utility while addressing associated limitations. Future research directions rapidly field proposed.

Язык: Английский

Процитировано

10

Plasma Biomarkers of Alzheimer’s Disease and Neurodegeneration According to Sociodemographic Characteristics and Chronic Health Conditions DOI Creative Commons
Heming Zheng, Zhiyong Wu, Michelle M. Mielke

и другие.

The Journal of Prevention of Alzheimer s Disease, Год журнала: 2024, Номер unknown

Опубликована: Янв. 1, 2024

Язык: Английский

Процитировано

5

Clinical Utility of an Alzheimer’s Disease Blood Test Among Cognitively Impaired Patients: Results from the Quality Improvement PrecivityAD2 (QUIP II) Clinician Survey Study DOI Creative Commons
Mark Monane, Demetrius M. Maraganore,

Robert G. Carlile

и другие.

Diagnostics, Год журнала: 2025, Номер 15(2), С. 167 - 167

Опубликована: Янв. 13, 2025

Objective: The objective of this study was to assess clinical decision-making associated with the use a multi-analyte blood biomarker (BBM) test among patients presenting signs or symptoms mild cognitive impairment dementia. Methods: Quality Improvement PrecivityAD2 (QUIP II) Clinician Survey (NCT06025877) evaluated utility PrecivityAD2™ in prospective, single cohort 203 Alzheimer’s disease (AD) other causes decline across 12 memory specialists. (C2N Diagnostics, St. Louis, MO) combines plasma Aβ42/Aβ40 ratio and p-tau217/np-tau217 (%p-tau217) measurements statistical algorithm yield an Amyloid Probability Score 2 (APS2) that informs on likelihood brain amyloid plaques. After receiving BBM results, clinicians completed surveys management strategies for each patient. Results: Patients had median age 74, 53% were female, 28% traditionally under-represented Black, Hispanic, Asian groups. composite primary endpoint, defined as change AD diagnostic certainty, drug therapy, additional evaluation pre- post-BBM testing, 75% (p < 0.0001 versus pre-specified threshold 20% clinically meaningful change). Anti-AD medication orders decreased negative APS2 increased positive 0.0001). Additional testing Conclusions: This can help specialists guide anti-AD therapies well rule out allow considerations.

Язык: Английский

Процитировано

0

High precision and cost-effective multiplex quantification of amyloid-β40, amyloid-β42, p181Tau, p217Tau, neurofilament light chain, and glial fibrillary acidic protein from plasma and serum DOI

Farshad Alishahi,

Christopher R. Beam, Margaret Gatz

и другие.

Journal of Alzheimer s Disease, Год журнала: 2025, Номер unknown

Опубликована: Май 13, 2025

Background Current methods to quantify blood biomarkers for Alzheimer's disease (AD) are expensive and not widely available. Objective To develop a low-cost, sensitive, accurate multiplex assay Aβ 40 , 42 p181Tau, p217Tau, NfL, GFAP in plasma serum based on available technology. Methods We used commercial antibodies GFAP, xMAP Luminex technology, developed the 5ADCSI these from serum. The utility of was tested matched cerebrospinal fluid (CSF) or cohort cognitively normal (CN: n = 35), with mild cognitive impairment (MCI: 17), AD (n 11) individuals. Results demonstrated high specificity sensitivity, excellent precision. In clinical samples, moderate strong correlation is observed between CSF 42/40 ( r 0.78 ), p181Tau/Aβ 0.57 p217Tau/Aβ 0.72 p181Tau 0.59 p217Tau 0.75 ). AUC receiver-operator characteristic curve differentiating CN plasma/serum 0.75, 0.80 0.95, 0.91 0.76, 0.81 0.73 0.78for respectively. Conclusions highly specific, accurate. wide availability base technology an advantage over other similar would allow cost-effective large-scale studies validation early diagnosis AD.

Язык: Английский

Процитировано

0

Mass spectrometry-based methods for biofluid biomarkers for progressive diseases: amyloid peptides and dystrophins DOI
Makoto Niwa, Kenta Noda, Yoshiyuki Kobayashi

и другие.

Bioanalysis, Год журнала: 2025, Номер unknown, С. 1 - 10

Опубликована: Июнь 4, 2025

Using two diseases, Alzheimer's disease and muscular dystrophy as examples, in which many resources have been invested, trends bioanalytical techniques for detection of peptide or protein biomarkers using mass spectrometry are reviewed Web Science database. Amyloid beta peptides used to detect disease, obtained by tryptic digestion dystrophin dystrophy. Based on the recent interest peripheral blood analysis, literature review was focused biofluid analysis. Both electrospray matrix-assisted laser desorption ionization were employed amyloid peptides, high-resolution ion mobility had introduced enhance selectivity. Immunoprecipitation often pretreatment; however, emergence combined use different solid-phase extraction mode observed. Regarding from dystrophin, there few reported applications biofluids, typically ionization-selected reaction monitoring with pretreatment employed. Validity propelled development methods attracting research interest. The analytical methodologies built proven field current interests increasing selectivity sensitivity, such differential parallel monitoring.

Язык: Английский

Процитировано

0

Dysregulation of Porphyromonas gingivalis Agmatine Deiminase Expression in Alzheimer’s Disease DOI

Asma Hamdi,

Sana Baroudi,

Alya Gharbi

и другие.

Current Alzheimer Research, Год журнала: 2024, Номер 21(4), С. 232 - 241

Опубликована: Апрель 1, 2024

Background: Alzheimer’s disease (AD) is the most prevalent neurodegenerative disorder, with a significant burden on global health. AD characterized by progressive cognitive decline and memory loss. Emerging research suggests potential link between periodontitis, specifically presence of oral bacteria such as Porphyromonas gingivalis (P. gingivalis), progression. P. produces an enzyme, Agmatine deiminase (AgD), which converts agmatine to N-carbamoyl putrescine (NCP), serving precursor essential polyamines. Recent studies have confirmed correlation disruptions in polyamine metabolism impairment. Objective: This study aims investigate dysregulation (PgAgD) context AD. Methods: Saliva samples were collected from total 54 individuals, including 27 patients healthy controls. The expression PgAgD gene was analyzed using quantitative Real-- Time PCR. Results: results showed decrease saliva compared downregulation found advanced stages periodontitis. Additionally, observed 30-item Mini-Mental State Examination (MMSE) score. Conclusion: These findings suggest that measuring could be noninvasive tool for monitoring progression aid early diagnosis Further needed validate our explore underlying mechanisms linking expression, pathophysiology.

Язык: Английский

Процитировано

0