Fluorescence Resonance Energy Transfer in Gelatin-Based MOF/N-CDs Films for Superior Photodynamic Antibacterial Packaging Materials

ACS Sustainable Chemistry & Engineering, Год журнала: 2025, Номер unknown

Опубликована: Фев. 14, 2025

Язык: Английский

New high accuracy diagnostics for avianAspergillus fumigatusinfection using Nanopore methylation sequencing of host cell-free DNA and machine learning prediction

Опубликована: Апрель 15, 2025

Abstract Avian aspergillosis is a detrimental fungal infection affecting wild and domestic birds yet sensitive antemortem diagnostics for early clinical infections are lacking. Here we present new Aspergillus fumigatus ( Af ) developed from cell-free DNA (cfDNA) methylation markers. Broiler chickens were experimentally infected with either Af, non- agent Escherichia coli or Gallibacterium anatis assigned as controls. Oxford Nanopore (ONT) sequencing was performed on serum cfDNA (n = 124), machine learning (ML) models trained infection-specific Three tests developed: A ‘High Accuracy’ test best performance (sensitivity: 100%, specificity: 89.2%) robustness (ROC-AUC: 0.92) well ‘Fast’- ‘In situ’ rapid turnaround PCR. Diagnostic accuracies 92.3%, 82.7%, 73.1%, respectively. In conclusion, using ML- host markers demonstrated high diagnostic comparable to microbial (mcfDNA) but without concern environmental contamination. Key highlights We three accuracy in that use (cfDNA). Differentially methylated regions (DMRs) detected by used train development of tests. The highest found 83 10 kilobases (KB) the glmnet algorithm ML model, which classified 92.3% blinded samples correctly. Fast designed cheap <1h adaptive sampling could correctly classify 82.7% 22 random forest (rf) model. An situ only four markers, envisioned simple methylation-specific PCR (MSP-PCR) assay, 73.1% samples. Reference values associated probabilities calculated each presented further evaluation.

Язык: Английский

Air Pollution-Linked Epigenetic Modifications in Placental DNA: Prognostic Potential for Identifying Future Foetal Anomalies

Reproductive Toxicology, Год журнала: 2024, Номер 129, С. 108675 - 108675

Опубликована: Июль 27, 2024

Язык: Английский