Journal of Global Antimicrobial Resistance, Год журнала: 2024, Номер unknown
Опубликована: Окт. 1, 2024
Язык: Английский
Veterinary Microbiology, Год журнала: 2024, Номер 298, С. 110282 - 110282
Опубликована: Окт. 16, 2024
Язык: Английский
Процитировано
1
Antimicrobial Agents and Chemotherapy, Год журнала: 2024, Номер 69(1)
Опубликована: Дек. 10, 2024
The global rise of carbapenem-resistant Klebsiella pneumoniae, including strains producing K. pneumoniae carbapenemase (KPC) types, poses a significant public health challenge due to their resistance critical antibiotics. Treatment options for infections caused by KPC-producing (KPC-KP) are increasingly limited, particularly as these develop last-line antibiotics such ceftazidime/avibactam and colistin. This study investigates the evolution antibiotic persistence in series clonally related ST11 KPC-KP isolated from single patient undergoing extended antimicrobial treatment. patient, 47-year-old male with history kidney transplantation, developed multiple lung during his hospital stay. Resistance colistin emerged treatment Key mechanisms identified included integration ISKpn26 into mgrB gene, leading inactivation resistance, emergence novel blaKPC-2 variants (blaKPC-71 blaKPC-179) that confer ceftazidime/avibactam. Despite development ceftazidime/avibactam-resistant strain following combination therapy, patient's clinical condition significantly improved. Phenotypic assays showed disruption resulted increased biofilm formation higher susceptibility phagocytosis. In mouse models, reduced virulence, colonization persistence, lower inflammatory response, suggesting facilitates transition acute infection colonization. highlights complex interplay between bacterial fitness, offering insights why some patients experience improvement despite severe drug incomplete clearance.
Язык: Английский
Процитировано
1
Frontiers in Cellular and Infection Microbiology, Год журнала: 2024, Номер 14
Опубликована: Авг. 19, 2024
Background Klebsiella pneumoniae is a major cause of hospital-acquired infections (HAIs), primarily spread through environmental contamination in hospitals. The effectiveness current chemical disinfectants waning due to emerging resistance, which poses hazards and fosters new resistance pathogens. Developing environmentally friendly effective against multidrug-resistant organisms increasingly important. Methods This study developed bacteriophage cocktail targeting two common carbapenem-resistant (CRKP) strains, ST11 KL47 KL64. was used as an adjunctive disinfectant hospital’s respiratory intensive care unit (RICU) via ultrasonic nebulization. Digital PCR quantify CRKP levels post-intervention. microbial community composition analyzed 16S rRNA sequencing assess the intervention’s impact on overall diversity. Results phage significantly reduced within first 24 hours post-treatment. While slight increase pathogen observed after hours, they remained lower than those treated with conventional disinfectants. showed decrease target pathogens’ relative abundance, while species diversity stable, confirming that phages selectively without disrupting ecological balance. Discussion findings highlight efficacy safety phage-based biocleaners sustainable alternative Phages reduce pathogens preserving diversity, making them promising tool for infection control.
Язык: Английский
Процитировано
0
Communications Biology, Год журнала: 2024, Номер 7(1)
Опубликована: Авг. 23, 2024
Clinical metagenomics (CMg) Nanopore sequencing can facilitate infectious disease diagnosis. In China, sub-lineages ST11-KL64 and ST11-KL47 Carbapenem-resistant Klebsiella pneumoniae (CRKP) are widely prevalent. We propose PathoTracker, a specially compiled database arranged method for strain feature identification in CMg samples CRKP traceability. A targeting high-prevalence horizontal gene transfer strains ST11-only distinguishing two China were created. To make the user-friendly, immediate downstream from raw metagenomic data, avoid need phylogenetic analysis scratch, we developed data methods. The methods included pre-performed analysis, gene-isolate-cluster index multilevel pan-genome reduced storage space by 10-fold random-access memory 52-fold compared with normal PathoTracker provide accurate fast strain-level after 1 h sequencing, allowing early warning of outbreaks. user-friendly page ( http://PathoTracker.pku.edu.cn/ ) was to online including feature, species identifications analyses. proposed this study will aid CMg. This constructed platform characterization clinical website analysis.
Язык: Английский
Процитировано
0
Journal of Global Antimicrobial Resistance, Год журнала: 2024, Номер unknown
Опубликована: Окт. 1, 2024
Язык: Английский
Процитировано
0