Alleviating effect of Lactobacillus fermentum E15 on hyperlipidemia and hepatic lipid metabolism in zebrafish fed by a high-fat diet through the production of short-chain fatty acids

Frontiers in Nutrition, Год журнала: 2025, Номер 12

Опубликована: Март 3, 2025

Introduction Hyperlipidemia is regarded as one of the crucial factors leading to atherosclerosis and other cardiovascular diseases. Gut microbiota plays an important role in regulating host lipid metabolism. Nevertheless, exact mechanisms behind this remain unclear. Methods In present study, a hyperlipidemic zebrafish model was established using high-cholesterol diet (HCD) evaluate anti-hyperlipidemic effects Lactobacillus fermentum E15 ( L. E15). Results showed that effectively reduced accumulation blood vessels liver HCD-fed larvae. Meanwhile, improved abnormal levels, normalized enzyme activity. Real-time quantitative polymerase chain reaction (RT-qPCR) analysis revealed downregulated expression sterol regulatory element-binding factor (SREBP-1), peroxisome proliferator-activated receptor-gamma (PPAR- γ ), fatty acid synthase (Fasn), while upregulated receptor-alpha α ). Additionally, metabolomic produced series short-chain acids (SCFAs), including acetic acid, propionic butyric isovaleric acid. Notably, contributed reduction droplet contrast, blocking G-protein coupled receptor 43 (GPR43) with pertussis toxin (PTX) abolished on reducing RT-qPCR results further suggested both promoted GPR43 leptin A, which inhibited by PTX. Conclusion These findings alleviates HCD-induced hyperlipidemia activating through SCFAs.

Язык: Английский

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Modulation of gut microbiota by probiotics to improve the efficacy of immunotherapy in hepatocellular carcinoma

Frontiers in Immunology, Год журнала: 2024, Номер 15

Опубликована: Ноя. 22, 2024

Hepatocellular carcinoma, a common malignancy of the digestive system, typically progresses through sequence hepatitis, liver fibrosis, cirrhosis and ultimately, tumor. The interaction between gut microbiota, portal venous system biliary tract, referred to as gut-liver axis, is crucial in understanding mechanisms that contribute progression hepatocellular carcinoma. Mechanisms implicated include dysbiosis, alterations microbial metabolites increased intestinal barrier permeability. Imbalances or contributes carcinoma by producing carcinogenic substances, disrupting balance immune altering metabolic processes, increasing Concurrently, accumulating evidence suggests microbiota has ability modulate antitumor responses affect efficacy cancer immunotherapies. As new effective strategy, immunotherapy offers significant potential for managing advanced stages with checkpoint inhibitors achieving advancements improving patients' survival. Probiotics play vital role promoting health preventing diseases modulating inflammation responses. Research indicates they are instrumental boosting modulation microbiota. This review explore relationship emergence assess contributions probiotics outline latest research findings, providing safer more cost-effective strategy prevention management

Язык: Английский

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Corn Peptides Alleviate Nonalcoholic Fatty Liver Fibrosis in Mice by Inhibiting NLRP3 Inflammasome Activation and Regulating Gut Microbiota

Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер 72(35), С. 19378 - 19394

Опубликована: Авг. 21, 2024

This study aimed to investigate the effects of corn gluten-derived soluble epoxide hydrolase (sEH) inhibitory peptides on nonalcoholic fatty liver fibrosis induced by a high-fat diet and carbon tetrachloride in mice. Mice treated with at doses 500 or 1000 mg/kg/d for 4 weeks exhibited reduced sEH activity serum liver, enhanced lipid metabolism, decreased accumulation oxidative stress. Corn effectively downregulated mRNA levels Pro-IL-1β, Pro-IL-18, NOD-like receptor protein 3 (NLRP3), ASC, Pro-caspase-1, Caspase-1, GSDMD liver. hepatoprotective effect inhibiting NLRP3 inflammasome activation was further validated H2O2-induced HepG2 cells. Moreover, restored composition gut microbiota promoted short-chain acid production. provides evidence that corn-derived have against suppressing modulating microbiota.

Язык: Английский

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The Impact of Bioactive Molecules from Probiotics on Child Health: A Comprehensive Review

Nutrients, Год журнала: 2024, Номер 16(21), С. 3706 - 3706

Опубликована: Окт. 30, 2024

: This review investigates the impact of bioactive molecules produced by probiotics on child health, focusing their roles in modulating gut microbiota, enhancing immune function, and supporting overall development. Key metabolites, including short-chain fatty acids (SCFAs), bacteriocins, exopolysaccharides (EPSs), vitamins, gamma-aminobutyric acid (GABA), are highlighted for ability to maintain regulate inflammation, support neurodevelopment.

Язык: Английский

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Neohesperidin Mitigates High-Fat-Diet-Induced Colitis In Vivo by Modulating Gut Microbiota and Enhancing SCFAs Synthesis

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(2), С. 534 - 534

Опубликована: Янв. 10, 2025

Previous research has consistently shown that high-fat diet (HFD) consumption can lead to the development of colonic inflammation. Neohesperidin (NHP), a naturally occurring flavanone glycoside in citrus fruits, anti-inflammatory properties. However, efficacy and mechanism NHP countering prolonged HFD-induced inflammation remains unclear. In this study, rats on HFD were intragastrically administered (i.g.) with for 12 consecutive weeks. Results indicate natural compound is effective reducing colorectal at doses 40-80 mg/kg body weight (BW) by i.g. administration, significant decreases markers such as TNF-α IL-1β levels. It also improved intestinal mucosal tissue integrity reduced HFD-stimulated via JAK2/STAT3 pathway. Furthermore, microbiota sequencing results show intervention significantly downregulated Firmicutes/Bacteroidetes ratio. This ratio closely related preventive role context glycolipid metabolism disorder. Compared fecal cultures from group, after 48 h vitro fermentation, those group had distinct composition notably higher concentrations SCFAs. Collectively, these observations suggest 80 BW possesses biological activities downregulating regulating flora promoting SCFAs formation.

Язык: Английский

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Camellia seed oil exerts a more gradual influence on the progression of high-fat diet induced NAFLD mice compared to corn oil: Insights from gut microbiota and metabolomics

Food Bioscience, Год журнала: 2025, Номер unknown, С. 105960 - 105960

Опубликована: Янв. 1, 2025

Язык: Английский

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Valerate enhances flesh hardness by inhibiting muscle proliferation and differentiation in Nile tilapia (Oreochromis niloticus): An in vivo and in vitro study

Aquaculture, Год журнала: 2025, Номер 600, С. 742222 - 742222

Опубликована: Янв. 27, 2025

Язык: Английский

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The impact of wild-boar-derived microbiota transplantation on piglet microbiota, metabolite profile, and gut proinflammatory cytokine production differs from sow-derived microbiota

Applied and Environmental Microbiology, Год журнала: 2025, Номер unknown

Опубликована: Фев. 4, 2025

ABSTRACT Colonization of co-evolved, species-specific microbes in early life plays a crucial role gastrointestinal development and immune function. This study hypothesized that modern pig production practices have resulted the loss co-evolved species critical symbiotic host-microbe interactions. To test this, we reintroduced from wild boars (WB) into conventional piglets to explore their colonization dynamics effects on gut microbial communities, metabolite profiles, responses. At postnatal day (PND) 21, 48 were assigned four treatment groups: (i) WB-derived mixed community (MMC), (ii) sow-derived MMC, (iii) combination WB sow MMC (Mix), or (iv) Control (PBS). Post-transplantation analyses at PND revealed distinct communities WB-inoculated compared with Controls, trends toward differentiation Sow but not Mix groups. more successful colonizing piglets, particularly group, where they competed Sow-derived microbes. group cecal digesta enriched Lactobacillus helveticus , mucosae pontis . Cecal analysis showed histamine, acetyl-ornithine, ornithine, citrulline, other metabolites, higher histamine levels linked abundance. exhibited lower IL-1β IL-6 groups, whereas reduced IFN-γ, IL-2, group. No differences weight gain, fecal scores, plasma cytokines observed, indicating no adverse effects. These findings support missing effectively colonize domestic may positively impact IMPORTANCE addresses growing concern over losing agricultural practices, production. The implementation strict biosecurity measures widespread antibiotic use farming systems disrupt interactions are essential for Our research demonstrates by reintroducing boar-derived these can successfully gut, influence composition, alter profiles responses without causing also suggest native fill an intestinal niche, impacting activation. lays groundwork future strategies enhance livestock health performance restoring natural populations produce immune-modulating metabolites.

Язык: Английский

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Exercise and Berberine Intervention Ameliorate High-Fat Diet-Induced MAFLD by Regulating Gut Microbiota and Hepatic Fatty Acid Beta-Oxidation

Journal of Inflammation Research, Год журнала: 2025, Номер Volume 18, С. 2837 - 2854

Опубликована: Фев. 1, 2025

Metabolic dysfunction-associated fatty liver disease (MAFLD) is a global concern. The gut microbiota and hepatic acid beta-oxidation have been shown to be important factors in the development of MAFLD. Independently, exercise berberine can significantly ameliorate symptoms MAFLD, although specific mechanism not clear; moreover, it known whether combination produces better therapeutic effect. Over an experimental period 17 weeks, effects exercise, berberine, combined (exercise/berberine) intervention on composition expression related proteins were examined. mice divided into five treatment groups: CON (control group, n = 10), HFD (high-fat diet, HFE diet + HFB HBE (high-fat-diet 10). dose BBR administered for oral gavage was 300 mg/kg, once per day, 8 weeks. Mice subjected treadmill 5 days week intensity increased gradually. Serological histopathological results showed that effectively improved lipid accumulation caused by high-fat diet. Analysis 16S rRNA three interventions restored species number MAFLD mice. functional prediction revealed significant differences beta-oxidation-related units among groups. Simultaneously, regulated ACOX1, HMGCS2, CPT-1α, with having more effect than each alone. Our findings indicate regulating beta-oxidation, suggesting their may potential therapy MALFD.

Язык: Английский

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Anticancer activity of Weizmannia coagulans MZY531on H22 tumor-bearing mice by regulating inflammation, autophagy-dependent apoptosis, and gut microbiota

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Март 10, 2025

Increasing studies have shown that the efficacy of Weizmannia coagulans in treating various cancers. We recently identified W. MZY531 with potent cell anti-proliferation and exhibiting apoptosis induction activities against mouse H22 hepatocellular carcinoma line.However, anti-cancer effect liver cancer vivo has not been verified. The objective this study was to assess anti-hepatoma on tumor-bearing mice underlying mechanism. results demonstrated reduced weight size tumor comparison model group. levels serum pro-inflammatory cytokines, including IL-1β, IL-6, IL-2 TNF-α were suppressed by administration. Immunofluorescence TUNEL analyses significantly increased number cleaved caspase-3 cells induced tissues. Importantly, activated AMPK/mTOR autophagy-dependent pathway, regulated TLR4/MyD88/TRAF-6/NF-κB JAK2/STAT3 inflammatory signaling pathways through mechanisms. Additionally, Fecal analysis capacity remodel gut microbiota carcinoma-infected mice. Collectively, experimental finding suggested exhibited prominent anticancer at least partly via reducing inflammation, inducing apoptosis, regulating

Язык: Английский

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