Ecotoxicology and Environmental Safety,
Год журнала:
2024,
Номер
287, С. 117271 - 117271
Опубликована: Ноя. 1, 2024
Tetrabromobisphenol
A
(TBBPA)
is
a
widely
used
flame
retardant.
TBBPA
persistent
pollutant
that
difficult
to
degrade
and
causes
sustained
pollution
the
environment.
has
been
detected
in
human
blood
tissues,
studies
indicate
it
various
toxicological
damages
tissues
cells.
To
date,
effects
of
on
chondrocytes
are
not
fully
understood.
Here,
we
evaluated
tissues.
For
this,
human-
mouse-derived
chondrocyte
models
were
analyze
TBBPA.
Physiological
concentrations
stimulate
chondrocytes.
Indirect
immunofluorescence,
flow
cytometry,
biochemical
assays
utilized
investigate
TBBPA's
impact
The
CCK8
experiment
indicated
reduced
proliferation.
ELISA
Western
blot
results
increased
inflammatory
factor
expression
We
also
found
caused
oxidative
stress
Mechanistic
study
showed
triggered
imbalance
homeostasis
calcium
ions,
leading
mitochondrial
depolarization,
which
induces
inflammation
In
vivo
model,
our
research
knee
joint
cartilage.
Safranin
O-Fast
Green
H&E
staining
revealed
cartilage
tissue
damage
following
treatment.
MMP9
MMP13
down-regulated
COL2
expression.
summary,
assessed
experimental
data
This
establishes
basis
for
future
impacts
Authorea (Authorea),
Год журнала:
2024,
Номер
unknown
Опубликована: Апрель 20, 2024
Background
and
Purpose:
Lycorine
is
an
alkaloid
that
was
in
the
bulb
of
genus
Lycoris.
It
has
properties
anti-inflammatory.
This
study
aimed
to
investigate
molecular
mechanism
by
which
lycorine
can
reduce
acute
lung
injury
(ALI).
Experimental
Approach:
ALI
model
established
intranasal
injection
lipopolysaccharide
(LPS).
In
vitro,
A549
cells
were
treated
with
LPS
pretreated
for
1
hour.
Key
Results:
The
results
showed
reduced
histopathological
changes
lung,
myeloperoxidase
(MPO)
activity,
production
inflammatory
cytokines
such
as
TNF-α,
IL-1β,
IL-6
mice.
dose-dependently
inhibited
IL-6.
also
transmission
TLR4/NF-κB
passway
LPS-stimulated
cells.
increased
cholesterol
efflux
through
activated
LXRα-ABCA1/ABCG
pathway.
a
good
binding
ability
LXRα.
After
adding
LXRα
inhibitor,
anti-inflammatory
effect
eliminated.
Conclusion
Implications:
induced
lipopolysaccharide.
related
up-regulation
pathway,
inhibits
TLR4-mediated
inflammation
increasing
reducing
TLR4
transport
lipid
rafts.
