A Method to Evaluate and Eliminate Fungal Contamination in Household Air Conditioners

Indoor Air, Год журнала: 2023, Номер 2023, С. 1 - 10

Опубликована: Май 22, 2023

Background. Allergic fungal airway diseases, such as asthma and allergic bronchopulmonary mycosis (ABPM), are often difficult to manage with medical treatment alone; therefore, environmental exposure should be accurately evaluated minimized. In the present study, we established a method evaluate eliminate contamination in household air conditioners (ACs). Methods. fall of 2020, an survey living rooms was conducted 17 Japanese residences patients ABPM or related diseases. Household ductless minisplit AC units were disassembled collect swab samples from internal parts (filter, heat exchanger, blower fan, vent), followed by high-pressure washing. Fungal abundance composition cleaning effluents ACs well house dust determined using quantitative PCR next-generation sequencing transcribed spacer 1 region, respectively. A weighted UniFrac distance calculated analyze similarity mycobiome among samples. Results. All interior contained high levels DNA, fans being most contaminated parts. Cladosporium Toxicocladosporium, Aureobasidium, Aspergillus, Rhodotorula, common fungi detected unit. High-pressure washing decreased over 99% all strongly correlated those effluents. Conclusion. Interior downstream exchangers major sites contamination, possibly polluting indoor residences. is highly effective for decontamination.

Язык: Английский

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Indoor Environment and Health Effects: Protocol of an Exploratory Panel Study among Young Adults in China (China IEHE Study)

Environment & Health, Год журнала: 2024, Номер 3(1), С. 26 - 39

Опубликована: Июль 11, 2024

Indoor environment and health have drawn public attention worldwide. However, the joint effects mechanisms of exposure to different types indoor environmental factors remain unclear. We established an exploratory panel study on among young adults in China (the IEHE Study) comprehensively investigate 3M issues, including multiple factors, effects, omics methods for mechanism exploration. This protocol aims systematically introduce entire Study. Eighty-one aged 18-28 years from a university adjacent traffic arteries Beijing were recruited followed up four times. Sham/real air purification intervention was simultaneously applied randomized crossover order. A broad range physical, chemical, biological characterized through real-time monitoring external internal analyses. Subclinical indices reflecting cardiopulmonary, sleep, cognitive repeatedly measured prospective Various biosamples fasting venous blood, morning urine, nasal mucosal lining fluid, exhaled breath condensate collected explore underlying mechanisms. The Study comes with enlightening framework future studies associated exploration multisystem exposure.

Язык: Английский

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The study of effective factors in sick building syndrome related to fungi and its control methods

Results in Engineering, Год журнала: 2024, Номер 23, С. 102703 - 102703

Опубликована: Авг. 8, 2024

Various biological agents including fungi are effective in the symptoms of sick building syndrome. Fungal growth and activity depend on environmental parameters, which causes different types to be observed indoor environment. In this study, relationship between syndrome fungal was reviewed. Articles were selected by applying a search protocol inclusion criteria. The health consequence environment such as allergies discussed three separate categories. consequences metabolites mycotoxins also Also, factors affecting humidity temperature discussed. Strategies control associated with defined congeries. Identifying caused them can useful using optimum method fungi. Assessment risk for residential office buildings effectiveness strategies improving physical conditions or disinfection reducing it considered future studies.

Язык: Английский

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Environmental impacts on childhood rhinitis: The role of green spaces, air pollutants, and indoor microbial communities in Taiyuan, a city in Northern China

Ecotoxicology and Environmental Safety, Год журнала: 2025, Номер 289, С. 117662 - 117662

Опубликована: Янв. 1, 2025

Rhinitis is one of the most common respiratory diseases, influenced by various environmental factors such as green space, air pollution and indoor microbiomes. However, their interactions combined effects have not been reported. We recruited 1121 preschool children from day care centers in a northern city China. Health demographic data were collected through questionnaires answered children's parents. Surrounding space was assessed Normalized Difference Vegetation Index (NDVI), Enhanced (EVI) land cover grassland proportion within 1500/3000 m. Ambient estimated using inverse distance weighted (IDW), microbiome classroom vacuum dust profiled bacterial 16S rRNA fungal ITS amplicon sequencing. Mixed-effect logistic regression revealed natural grassland, leaf-off total negatively associated with current rhinitis. Stratified analysis indicated that greater exposure reduced rhinitis at high levels pollution. Additionally, also protects against tobacco smoke home. Indoor showed Haemophilus Dolosigranulum enriched low-rhinitis centers, while Amaricoccus, Blautia Mycosphaerella high-rhinitis centers. Mediation did significant mediating on relationship between This first study to reveal rhinitis, providing new insights into how collectively influence health children.

Язык: Английский

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Metagenomic assessment and next-generation sequencing (NGS) in indoor air microbiome analysis: A short review of current evidence

AIP conference proceedings, Год журнала: 2025, Номер 3275, С. 060002 - 060002

Опубликована: Янв. 1, 2025

Язык: Английский

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Integrated Analysis of Indoor Air Quality and Fungal Microbiota in Educational Heritage Buildings: Implications for Health and Sustainability

Sustainability, Год журнала: 2025, Номер 17(3), С. 1091 - 1091

Опубликована: Янв. 29, 2025

Indoor air quality is paramount for the health and well-being of individuals, especially in enclosed spaces like office buildings, schools, hospitals, homes where people spend a significant amount time. Ensuring good indoor not only essential reducing symptoms such as headaches, eye respiratory irritation, fatigue, difficulty concentration, but it also key component sustainable building practices aimed at promoting long-term environmental balance. This study aims to explore impact microclimate fungal microbiota on cognitive performance occupants university classroom, which part cultural heritage building. The research delves into various microclimatic parameters, including temperature, relative humidity, CO2, volatile organic compounds, O2, particulate matters (PM2.5 PM10), understand their influence development manifestation associated with Sick Building Syndrome. Over course year-long investigation, microbiological samples were collected, revealing presence 19 species, Cladosporium, Alternaria, Aureobasidium being most prevalent genera. These species found thrive an environment characterized by inadequate ventilation, posing potential risks occupants, allergic reactions infections. Microclimatic parameter values mean temperature 22.9 °C humidity 38.5% indicated moderate conditions proliferation, occasional high levels PM2.5 CO2 periods poor quality, negatively influencing comfort occupants. questionnaires completed 190 students showed that 51.5% reported 44.2% frequent sneezing, 43.7% severe linking these increased PM2.5. novelty lies integrated approach assessment educational building, highlighting need improved ventilation management enhance performance, while emphasizing balances occupant preservation heritage.

Язык: Английский

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Potential effects of indoor microbiome characteristics on health: A systematic review

Public Health, Год журнала: 2025, Номер 240, С. 137 - 147

Опубликована: Фев. 4, 2025

Язык: Английский

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A Multicenter Exploration of Sick Building Syndrome Symptoms in Malaysian Schools: Indoor Pollutants, Microbial Taxa, and Metabolites

Metabolites, Год журнала: 2025, Номер 15(2), С. 111 - 111

Опубликована: Фев. 10, 2025

Background: The role of the indoor microbiome in sick building syndrome (SBS) is well-recognized, yet prior studies have been limited to single-center analyses, limiting a broader understanding and applicability their findings. Methods: We conducted multicenter metabolome investigation for SBS, involving 1139 middle school students across three regions Malaysia (Johor Bahru, Terengganu, Penang). Using high-throughput amplicon sequencing untargeted LC-MS, metabolites were characterized from classroom dust samples. Results: study found that prevalence SBS symptoms was high all centers (51.0% 54.6%). Environmental characteristics, including NO2 CO2 concentrations total weight dust, positively associated with (p < 0.01, linear regression). Curtobacterium Terengganu negatively Clostridium perfringens Johor Bahru FDR 0.05). Whereas identified fungal taxa, an uncharacterized uc_f_Auriculariaceae_sp., Duportella kuehneroides, Wallemia mellicola, 0.05) Terengganu. Mediation analysis revealed adverse health effects on partially mediated by increased abundance uc_f_Auriculariaceae_sp. 0.05, effect 51.40%). Additionally, potential protective (S-adenosylmethionine, N-acetylserotonin, sphinganine, 4-hydroxy-2-quinolone, (2E,4Z,8E)-Colneleic acid) mainly derived environmental microorganisms, conferring against nasal tiredness. In contrast, synthetic chemicals higher symptoms, inducing eye symptoms. Conclusions: This emphasizes both significance fostering balanced microbiome/metabolite necessity reduce exposure deleterious substances, providing new insights future targeted intervention strategies.

Язык: Английский

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Prediction of daily childhood asthma exacerbation from ambient meteorological, environmental risk factors and respiratory viruses, Philadelphia, PA, 2011 to 2016

Environmental Science and Pollution Research, Год журнала: 2025, Номер unknown

Опубликована: Фев. 19, 2025

Abstract Childhood asthma exacerbation has multiple risk factors that occur concurrently in the environment — including extreme meteorological conditions, air pollution, aeroallergens, and respiratory virus infections. Few studies have predicted based on time-varying environmental factors, together. In this study, we constructed an autoregressive integrated moving average (ARIMA) model to predict “high-risk” days for childhood Philadelphia, PA from 2011 2016, during aeroallergen season of each year, using a total 28,540 case events identified electronic health record (EHR) data. We selected predictors quantile weighted sum regression (gQWS), incorporating temporal lags season-stratification (early- vs. late-season), which were entered subsequently into multivariable ARIMA models. found daily nitrogen dioxide (NO 2 ), as well monthly rhinovirus syncytial (RSV) infection levels, higher days, compared with lower risk. The performed better late-season (July October) than early (March June). Future work continued research is needed facilitate local guidelines pertaining exacerbation.

Язык: Английский

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A Sustainable Combined Approach to Control the Microbial Bioburden in the School Environment

Microorganisms, Год журнала: 2025, Номер 13(4), С. 791 - 791

Опубликована: Март 30, 2025

The indoor microbiome is a dynamic ecosystem including pathogens that can impact human health. In this regard, the school environment represents main living space of humans for many years, and an unhealthy significantly condition students’ School rooms suffer from insufficient ventilation use building materials may favor pathogen contamination, mostly sanitized by conventional chemical-based methods, which pollution, have temporary effects, induce selection antimicrobial resistance (AMR) in persistent microbes. search sustainable effective methods to improve healthiness classroom environment, pre–post case–control study was performed Italian high school. Over year, different interventions were sequentially placed evaluated their on bioburden air quality, introduction plants, mechanical system, probiotic-based sanitation (PBS) substitution chemical sanitation. Through continuous microbial monitoring enrolled rooms, via culture-dependent -independent remarkable level detected at baseline (around 12,000 20,000 CFU/m2, before after classes, respectively), composed Staphylococcus spp. fungi. Some decrease fungal contamination observed following plants. Still, most significant associated AMR PBS, decreased >80% (p < 0.001) up 3 Log10 compared controls. Collected data support combined strategies quality confirm PBS effectively control spread not only sanitary environments.

Язык: Английский

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