Pharmaceuticals,
Год журнала:
2024,
Номер
17(12), С. 1678 - 1678
Опубликована: Дек. 12, 2024
Background/Objectives:
This
study
evaluates
the
effect
of
Mn(II)
and
Co(II)
ions
on
production
anti-Candida
metabolites
by
endophytic
fungus
Aspergillus
sp.,
isolated
from
Dizygostemon
riparius.
The
objective
was
to
identify
metal-induced
secondary
with
antifungal
potential
against
drug-resistant
Candida
species.
Methods:
sp.
cultivated
in
Czapek
agar
supplemented
MnCl₂
(400
µM)
or
CoCl₂
(200
µM).
Metabolite
profiles
were
analyzed
using
UHPLC-DAD
LC-ESI-HRMS,
followed
structural
elucidation
via
NMR.
Antifungal
biofilm
inhibition
activities
tested
albicans
parapsilosis.
Toxicity
assessed
Tenebrio
molitor
larvae.
Results:
Key
metabolites,
including
pyrophen,
penicillquei
B,
fonsecinone
demonstrated
activity
MIC
values
4.37–280.61
µg/mL.
Fonsecinone
B
exhibited
superior
inhibition,
surpassing
fluconazole
reducing
biomass
viability.
In
vivo
assays
showed
low
toxicity,
survival
rates
above
80%
at
2×
MIC/kg.
Conclusions:
significantly
modulated
emerged
as
a
promising
candidate
for
therapy
due
its
potent
toxicity.
These
findings
support
further
investigation
into
therapeutic
fungal
combating
infections.
Eco-Environment & Health,
Год журнала:
2025,
Номер
unknown, С. 100136 - 100136
Опубликована: Янв. 1, 2025
Microplastics
(MPs)
and
antibiotic
resistance
genes
(ARGs)
pose
significant
challenges
to
the
One
Health
framework
due
their
intricate
multifaceted
ecological
environmental
impacts.
However,
understanding
of
how
MP
properties
influence
ARG
prevalence
in
mariculture
sediments
remains
limited.
Herein,
polystyrene
(PS)
polyvinyl
chloride
(PVC)
MPs
with
different
sizes
(20-120
μm
0.5-2.0
mm)
were
selected
evaluate
impacts
underlying
mechanisms
driving
ARGs
dissemination.
The
results
showed
that
PS
PVC
increased
relative
abundance
by
1.41-2.50-fold
2.01-2.84-fold,
respectively,
compared
control,
particularly
high-risk
genes.
polymer
type
effect
was
identified
as
more
influential
than
size
sediment
resistome
evolution.
shifted
microbial
community
assembly
from
stochastic
deterministic
processes,
thus
enriching
host
pathogens.
Furthermore,
highly
hydrophobic
not
only
recruited
bacteria
colonization
but
also
facilitated
exchange
within
plastisphere.
exogenous
additives
released
(e.g.,
heavy
metals,
bisphenol
A,
tridecyl
ester)
particles
synergistically
promoted
conjugative
transfer
inducing
oxidative
stress
enhancing
cell
membrane
permeability.
These
findings
revealed
characteristics
spread
marine
benthic
ecosystems,
underscoring
importance
mitigating
pollution
maintain
ecosystem
health,
prevent
zoonotic
diseases,
balance
global
health.
Analytical and Bioanalytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 25, 2025
Abstract
When
performing
effect
studies
to
investigate
the
impact
of
microplastic
(MP)
on
cell
lines,
algae,
or
daphnia,
it
is
advantageous
if
such
experiments
can
be
performed
without
use
surfactants.
The
need
for
surfactants
arises
from
fact
that
finely
milled
pristine
MP
particles
generally
are
hydrophobic.
Methods
preparation
larger
amounts
hydrophilic
and
hence
artificially
aged
approaches
their
characterization
high
importance.
Here
we
present
methods
age
polyethylene
terephthalate
(PET)
low-density
(PE)
using
alkaline
acidic
treatments
reproducibly
result
in
large
quantities
below
5
µm
with
considerably
increased
hydrophilicity.
were
characterized
particle
counting
by
single-particle
extinction
scattering
(SPES),
size
laser
diffraction
measurements,
zeta
potential
electrophoretic
light
scattering,
hydrophobicity
index
(Hy)
through
dark-field
(DF)
microscopy,
chemical
composition
inductively
coupled
plasma-mass
spectrometry
(ICP-MS),
Fourier
transform
infrared
(FTIR)
Raman
microscopy.
values
obtained
should
allow
as
qualitative
reference
materials
(RMs)
an
ordinal
property.
Evidence
maintained
integrity
hydrophilicity
(in
powder
form)
over
time
was
measurements
a
33-month
interval.
Uniformity
subsampling
respect
number
concentration
suspensions
within
10-day
period
also
investigated.
It
provided
evidence
possibility
reproducible
spiking
specific
relative
standard
deviations
(RSDs)
6.2
13.6%.
For
development
future
microplastics,
they
property
(artificial
Hy-index)
nominal
(identity
PET
PE
based
spectral
matching).
Graphical
Sustainability,
Год журнала:
2025,
Номер
17(3), С. 1093 - 1093
Опубликована: Янв. 29, 2025
Antibiotic
resistance
(AR)
is
recognized
by
the
World
Health
Organization
as
a
major
threat
to
human
health,
and
recent
studies
highlight
role
of
microplastics
(MPs)
in
its
spread.
MPs
environment
may
act
vectors
for
antibiotic-resistant
bacteria
(ARB)
antibiotic
genes
(ARGs).
Bacterial
communities
on
plastisphere,
surface
MPs,
are
influenced
plastic
properties,
allowing
ARB
colonize
form
biofilms.
These
biofilms
facilitate
transfer
ARGs
within
microbial
communities.
This
study
analyzed
data
from
LUCAS
soil
dataset
(885
samples
across
EU
countries)
using
Emu
tool
characterize
at
genus/species
level.
Functional
annotation
via
PICRUSt2,
supported
custom
output
formatting,
revealed
significant
correlations
between
genera
Solirubrobacter,
Bradyrhizobium,
Nocardioides,
Bacillus
with
pathways
linked
microplastic
degradation
resistance.
were
consistently
present
various
types
(woodland,
grassland,
cropland),
suggesting
their
potential
bioindicators
health
relation
MP
pollution.
The
findings
underscore
hotspots
ARGs,
offering
new
insights
into
identification
monitoring
ecological
impacts
related
contamination.
