Hydrogel Microneedle Patches Loaded with Stem Cell Mitochondria-Enriched Microvesicles Boost the Chronic Wound Healing

ACS Nano, Год журнала: 2024, Номер 18(39), С. 26733 - 26750

Опубликована: Сен. 6, 2024

Rescuing or compensating mitochondrial function represents a promising therapeutic avenue for radiation-induced chronic wounds. Adult stem cell efficacies are primarily dependent on the paracrine secretion of mitochondria-containing extracellular vesicles (EVs). However, effective strategies addressing quantity mitochondria and mitochondria-delivery system lacking. Thus, in this study, we aimed to design an hydrogel microneedle patch (MNP) loaded with cell-derived mitochondria-rich EVs gradually release deliver into wound tissues boost healing. We, first, used metformin enhance biogenesis thereby increasing (termed "Met-EVs") adipose-derived cells. To verify effects Met-EVs, established

Язык: Английский

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Call to action to properly utilize electron microscopy to measure organelles to monitor disease

European Journal of Cell Biology, Год журнала: 2023, Номер 102(4), С. 151365 - 151365

Опубликована: Окт. 16, 2023

This review provides an overview of the current methods for quantifying mitochondrial ultrastructure, including cristae morphology, contact sites, and recycling machinery a guide to utilizing electron microscopy effectively measure these organelles. Quantitative analysis ultrastructure is essential understanding biology developing therapeutic strategies mitochondrial-related diseases. Techniques such as transmission (TEM) serial block face-scanning microscopy, well how they can be combined with other techniques confocal super-resolution correlative light are discussed. Beyond their limitations challenges, we also offer specific magnifications that may best suited TEM mitochondrial, endoplasmic reticulum, machinery. Finally, perspectives on future quantification offered.

Язык: Английский

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Precise subcellular targeting approaches for organelle-related disorders

Advanced Drug Delivery Reviews, Год журнала: 2024, Номер 212, С. 115411 - 115411

Опубликована: Июль 19, 2024

Язык: Английский

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Protective effects of resveratrol against PM2.5-induced damage in hNSCs and its mitigation of PM2.5-induced mitochondrial dysfunction in a 3D scaffold system

Neuroscience, Год журнала: 2025, Номер unknown

Опубликована: Фев. 1, 2025

Язык: Английский

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The role of homogenization cycles and Poloxamer 188 on the quality of mitochondria isolated for use in mitochondrial transplantation therapy

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Янв. 27, 2025

Язык: Английский

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Mitochondrial activity and steroid secretion in mouse ovarian granulosa cells are suppressed by a PFAS mixture

Toxicology, Год журнала: 2025, Номер 512, С. 154083 - 154083

Опубликована: Фев. 9, 2025

The accumulation of a number per- and polyfluoroalkyl substances (PFASs) in ovarian follicular fluid (FF) has been documented, raising serious questions about their impact on female fertility. Here, we tested the hypothesis that mixture PFASs acts paracrine manner granulosa cells (GCs) as metabolism-disrupting chemical. We selected perfluorooctane sulfonate (PFOS; 22.4ng/mL), perfluorooctanoic acid (PFOA; 14.5ng/mL), perfluorohexane (PFHxS; 21.3ng/mL), perfluorodecanoic (PFDA; 0.9ng/mL), perfluoroheptane sulphonate (PFHpA; 0.6ng/mL), perfluoroundecanoic (PFUnDA; 0.4ng/mL), perfluorononanoic (PFNA; 2ng/mL), which were most commonly detected FF women undergoing assisted reproductive technology treatment. Exposure mouse GCs to PFAS decreased amount active mitochondria mitochondrial membrane potential, correlated with reduction ATP production inhibition oxidative phosphorylation (OXPHOS). At same time, expression membrane-associated steroidogenic enzyme 3-beta-hydroxysteroid dehydrogenase (3βHSD) major steroids progesterone estradiol decreased. In addition, activity superoxide dismutase 1 (SOD1), an neutralizes reactive oxygen species (ROS), while ROS levels lipid peroxidation increased without cell death, indicating had subtoxic effects. Our results show mixtures, at concentrations similar those found human led GC dysfunction by impairing function steroid secretions therefore may have implications for health.

Язык: Английский

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Exploring cellular dynamics: Engineered fluorescent carbon dots for organelle staining and cellular response analysis

Coordination Chemistry Reviews, Год журнала: 2025, Номер 534, С. 216552 - 216552

Опубликована: Март 3, 2025

Язык: Английский

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Complosome Regulates Hematopoiesis at the Mitochondria Level

Stem Cell Reviews and Reports, Год журнала: 2025, Номер unknown

Опубликована: Март 7, 2025

The intracellular complement network, known as the complosome, regulates lymphocyte biology, which is well established. Recently, however, we demonstrated that complosome also expressed in hematopoietic stem/progenitor cells (HSPCs) addition to lymphocytes. In our previous work, murine lineage-negative (Lin-) bone marrow (BM) mononuclear (BMMNC) from mice lacking C3 and C5 proteins displayed different responses stress. Specifically, while C3-KO were more sensitive oxidative stress, C5-KO showed greater resistance. To explore this intriguing observation at metabolic level, evaluated anaerobic aerobic glycolysis, along with mitochondrial function, Lin- BMMNC purified C3-KO, C5-KO, C5aR1-KO mice. We found complosome-deficient animals under steady-state conditions exhibited elevated lactate production enhanced dehydrogenase (LDH) release, indicating their reliance on glycolysis. Interestingly, uptake of a glucose fluorescent analog (2-NBDG) increased but decreased compared wild-type (WT) Meanwhile, total ATP cells, unlike C5aR1 mice, was reduced did not change significantly after exposure mitochondrial-damaging agent hydrogen peroxide (H2O2). This suggests dependence glycolysis than cells. Finally, assessed integrity membranes studied using MitoTracker green deep red assays. Compared WT observed mitochondria mutant Lin-BMMNC accumulated fewer probes, presence defects these

Язык: Английский

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Autophagy modulation effect on homotypic transfer of intracellular components via tunneling nanotubes in mesenchymal stem cells

Stem Cell Research & Therapy, Год журнала: 2024, Номер 15(1)

Опубликована: Июль 2, 2024

Abstract Background Recent studies have proved the role of autophagy in mesenchymal stem cell (MSCs) function and regenerative properties. How by which mechanism modulation can affect juxtacrine interaction MSCs should be addressed. Here, was investigated formation tunneling nanotubes (TNTs) homotypic mitochondrial donation. Methods were incubated with 15 µM Metformin (Met) and/or 3 3-methyladenine (3-MA) for 48 h. The TNTs assessed using bright-field SEM images. mitochondria density ΔΨ values monitored flow cytometry analysis. Using RT-PCR protein array, close shared mediators between autophagy, apoptosis, Wnt signaling pathways also monitored. total fatty acid profile gas chromatography. Result Data indicated increase TNT length number, along other projections after induction while these features blunted 3-MA-treated ( p < 0.05). Western blotting revealed significant reduction Rab8 p-FAK 0.05), indicating inhibition assembly vesicle transport. Likewise, stimulation increased autophagic flux membrane integrity compared to MSCs. Despite findings, levels Miro1 2 unchanged inhibition/stimulation > We found that protein, transcription several related apoptosis involved different bioactivities. confirmed profound mono polyunsaturated/saturated ratio exposed stimulator. Conclusions In summary, could is required Thus, creates a promising perspective efficiency cell-based therapies.

Язык: Английский

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Tetrahymena ATG8 homologs, TtATG8A and TtATG8B, are responsible for mitochondrial degradation induced by starvation

mBio, Год журнала: 2025, Номер unknown

Опубликована: Май 15, 2025

ABSTRACT The majority of heterotrophic unicellular eukaryotes have evolved mechanisms to survive periods starvation, allowing them endure until conditions are favorable for regrowth. ciliate Tetrahymena exhibits active swimming behavior in water, preying on microorganisms and growing exponentially at a rate 0.5–0.75 h⁻¹ under optimal conditions. In this organism, numerous mitochondria localize the cell cortex along ciliary rows, likely ensuring an efficient ATP supply necessary vigorous movement. Although mitochondrial reduction occurs immediately underlying mechanism remains unknown. Here, we demonstrated that autophagy is responsible thermophila . Among five T. ATG8 homologs, TtATG8A TtATG8B formed granule- cup-shaped structures response starvation. Fluorescent microscopy further showed associate with mitochondria. Moreover, correlative light electron analysis revealed colocalized or were engulfed by autophagosomes displayed abnormal appearances disrupted cristae structures. Additionally, repression expression significantly attenuated starvation-induced reduction. These findings suggest TtATG8A- TtATG8B-mediated key starved IMPORTANCE This study first comprehensive description degradation process nutrient starvation It well known surface structure ciliates consists elaborate spatial arrangement microtubule networks associated repetitive pattern inherited next generation cells like genetic information. Our provide basis understanding how maintain adequate amount nutritional Furthermore, successfully usefulness as experimental system studying quality control turnover. Further studies will facilitate comparative among diverse biological systems other than opisthokonta (yeast, cultured cells, etc.) their

Язык: Английский

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