Continuous self-repair protects vimentin intermediate filaments from fragmentation DOI Creative Commons
Quang D. Tran, Martin Lenz, Hugo Wioland

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Сен. 3, 2024

Abstract Intermediate filaments are key regulators of cell mechanics. Vimentin, a type intermediate filament expressed in mesenchymal cells and involved migration, forms dense network the cytoplasm that is constantly remodeled through transport, elongation/shortening, subunit exchange. While it known elongation involves end-to-end annealing, unclear how reverse process shortening by fragmentation occurs. Here, we use combination vitro reconstitution probed fluorescence imaging theoretical modeling to uncover molecular mechanism breakage. We first show vimentin composed two layers subunits, half which exchangeable immobile. also subunits tetramers. further reveal continuous self-repair soluble pool tetramers equilibrium with essential maintain integrity. Filaments break as consequence local fluctuations number per cross-section induced constant exchange determine tends if about four removed from same cross-section. Finally, analyze dynamics association/dissociation estimate binding energy tetramer complete versus partially disassembled filament. Our results provide comprehensive description turnover link between fragmentation. SIGNIFICANCE STATEMENT filaments, including vimentin, component cytoskeleton, for Inside cell, fulfill its functions. In particular, elongate fragment, but this breakage was unknown. Here along length, could locally weaken physical understanding mechanisms regulating feature determining dynamic organization both healthy diseased assembly disrupted.

Язык: Английский

Cytoskeletal crosstalk: A focus on intermediate filaments DOI Creative Commons
Lucas Pradeau-Phélut, Sandrine Étienne-Manneville

Current Opinion in Cell Biology, Год журнала: 2024, Номер 87, С. 102325 - 102325

Опубликована: Фев. 14, 2024

The cytoskeleton, comprising actin microfilaments, microtubules, and intermediate filaments, is crucial for cell motility tissue integrity. While prior studies largely focused on individual cytoskeletal networks, recent research underscores the interconnected nature of these systems in fundamental cellular functions like adhesion, migration, division. Understanding coordination distinct networks both time space essential. This review synthesizes current findings intricate interplay between emphasizing pivotal role filaments. Notably, filaments engage extensive crosstalk with microfilaments microtubules through direct molecular interactions, linkers, motors that form bridges, as well via more complex regulation intracellular signaling.

Язык: Английский

Процитировано

12
Structural determinants of intermediate filament mechanics DOI Creative Commons
Zanetta Kechagia,

Matthias Eibauer,

Ohad Medalia

и другие.

Current Opinion in Cell Biology, Год журнала: 2024, Номер 89, С. 102375 - 102375

Опубликована: Июнь 7, 2024

Intermediate filaments (IFs) are integral to the cell cytoskeleton, supporting cellular mechanical stability. Unlike other cytoskeletal components, detailed structure of assembled IFs has yet be resolved. This review highlights new insights, linking complex IF hierarchical assembly their properties and impact on functions. While we focus vimentin IFs, draw comparisons keratins, showcasing distinctive structural features that underlie unique responses.

Язык: Английский

Процитировано

5
How cytoskeletal crosstalk makes cells move: Bridging cell-free and cell studies DOI Creative Commons
James P. Conboy, Irene Istúriz Petitjean, Anouk van der Net

и другие.

Biophysics Reviews, Год журнала: 2024, Номер 5(2)

Опубликована: Июнь 1, 2024

Cell migration is a fundamental process for life and highly dependent on the dynamical mechanical properties of cytoskeleton. Intensive physical biochemical crosstalk among actin, microtubules, intermediate filaments ensures their coordination to facilitate enable migration. In this review, we discuss different aspects that govern cell provide, each aspect, novel perspective by juxtaposing two complementary approaches biophysical study cytoskeletal crosstalk: live-cell studies (often referred as top-down studies) cell-free bottom-up studies). We summarize main findings from both experimental approaches, provide our bridging perspectives address open questions how governs makes cells move.

Язык: Английский

Процитировано

3
Actin and vimentin jointly control cell viscoelasticity and compression stiffening DOI Creative Commons
James P. Conboy,

Mathilde G. Lettinga,

Pouyan E. Boukany

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Янв. 2, 2025

Abstract The mechanical properties of cells are governed by the cytoskeleton, a dynamic network actin filaments, intermediate and microtubules. Understanding individual collective contributions these three different cytoskeletal elements is essential to elucidate how maintain integrity during deformation. Here we use custom single-cell rheometer identify distinct vimentin viscoelastic nonlinear elastic response uniaxial compression. We used mouse embryonic fibroblasts (MEFs) isolated from wild type (WT) knockout (vim -/-) mice in combination with chemical treatments manipulate polymerization contractility. show through small amplitude oscillatory measurements strain ramp tests that vimentin, often overlooked cellular mechanics, plays role comparable maintaining cell stiffness resisting large compressive forces. However, appears be more important than determining energy dissipation. Finally comparing enucleated compression stiffening originates while nucleus play little this. Our findings provide insight into networks collectively determine cells, providing basis understand cytoskeleton ability resist external as well internal Significance statement A cell’s stress largely networks, but their relative contribution viscoelasticity deformations poorly characterized. reveal have an almost equal strain-stiffen under This work underscores cytoskeleton’s central mechanics synergy between framework for understanding components coordinate structural respond environments.

Язык: Английский

Процитировано

0
Continuous self-repair protects vimentin intermediate filaments from fragmentation DOI Creative Commons
Quang D. Tran, Martin Lenz, Hugo Wioland

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Сен. 3, 2024

Abstract Intermediate filaments are key regulators of cell mechanics. Vimentin, a type intermediate filament expressed in mesenchymal cells and involved migration, forms dense network the cytoplasm that is constantly remodeled through transport, elongation/shortening, subunit exchange. While it known elongation involves end-to-end annealing, unclear how reverse process shortening by fragmentation occurs. Here, we use combination vitro reconstitution probed fluorescence imaging theoretical modeling to uncover molecular mechanism breakage. We first show vimentin composed two layers subunits, half which exchangeable immobile. also subunits tetramers. further reveal continuous self-repair soluble pool tetramers equilibrium with essential maintain integrity. Filaments break as consequence local fluctuations number per cross-section induced constant exchange determine tends if about four removed from same cross-section. Finally, analyze dynamics association/dissociation estimate binding energy tetramer complete versus partially disassembled filament. Our results provide comprehensive description turnover link between fragmentation. SIGNIFICANCE STATEMENT filaments, including vimentin, component cytoskeleton, for Inside cell, fulfill its functions. In particular, elongate fragment, but this breakage was unknown. Here along length, could locally weaken physical understanding mechanisms regulating feature determining dynamic organization both healthy diseased assembly disrupted.

Язык: Английский

Процитировано

2