bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 28, 2024
Abstract
The
biosynthesis
of
silver
nanoparticles
has
recently
emerged
as
a
promising
approach
in
nanomedicine,
particularly
for
targeted
therapeutic
applications.
Green
synthesized
(plant-based)
have
been
shown
to
offer
enhanced
reduction
efficiency,
greater
bioavailability,
and
improved
stability
compared
synthetic
nanoparticles.
Here,
we
report
the
green
synthesis
(AgNPs)
using
Magnolia
alba
leaf
extract.
formation
these
Magnolia-derived
(MAgNPs)
was
verified
through
UV-Vis
spectroscopy
further
characterized
by
scanning
electron
microscopy
(SEM)
which
showed
that
MAgNPs
mean
diameter
40
nm
spherical
morphology.
antibacterial
efficacy
MAgNPs,
evaluated
well
diffusion
method,
significant
activity
against
E.
coli
,
Klebsiella
pneumoniae
Pseudomonas
aeruginosa
Enterococcus
faecalis,
strains
methicillin-resistant
-sensitive
Staphylococcus
aureus.
Disc
plaque
assays
demonstrated
notable
antifungal
Candida
albicans
antiviral
bacteriophage
T7.
In
vitro
studies
with
HCT-116
human
colon
cancer
cells,
exhibited
bi-phasic
inhibition
cell
growth.
These
effects
were
than
magnolia
extracts
alone,
confirming
nanoparticles’
bioactive
efficacy.
findings
suggest
possess
antimicrobial
anticancer
activities,
indicating
their
potential
agents
certain
infections
cancers.
Antioxidant
indicated
exhibit
antioxidant
extract
alone.
Results
may
promise
antioxidants
treating
free
radical-induced
disorders.
Additionally,
efficient
photocatalytic
degradation
azo
bond
methyl
orange
within
30
minutes,
suggesting
they
provide
sustainable
types
environmental
pollution.
To
our
knowledge,
this
is
first
examination
properties,
killing
inhibitory
on
various
bacteria,
fungi,
bacteriophages,
cancer.
Green Processing and Synthesis,
Год журнала:
2025,
Номер
14(1)
Опубликована: Янв. 1, 2025
Abstract
This
study
optimizes
the
green
synthesis
of
silver
nanoparticles
(AgNPs)
using
seed
skin
extract
guarana
(
Paullinia
cupana
)
as
a
natural
stabilizing
and
reducing
agent.
Employing
Taguchi
design,
nine
experiments
were
conducted
across
three
levels
for
four
key
factors:
reaction
temperature,
pH,
nitrate
concentration,
concentration
to
minimize
AgNP
size.
Optimal
conditions
–
pH
11,
5
mM
nitrate,
50°C
1%
(m/v)
produced
quasi-spherical
AgNPs
with
an
average
size
∼26
nm.
Chemical
analysis
revealed
caffeine
main
organic
compound
potassium
oxide
primary
inorganic
component.
UV-vis
spectra
showed
absorption
peak
at
438
nm,
X-ray
diffraction
confirmed
typical
peaks.
Further
identified
polyphenols,
alkaloids,
flavonoids
agents.
A
high
(180.0
±
0.2
ppm)
was
through
ICP-OES,
demonstrated
significant
antibacterial
activity
against
Escherichia
coli
Staphylococcus
aureus
,
minimum
inhibitory
50
μg·mL
−1
.
underscores
sustainability
promising
alternative
traditional
nanoparticle
production
methods.
Materials Technology,
Год журнала:
2024,
Номер
40(1)
Опубликована: Дек. 16, 2024
Global
cancer
mortality
is
rising
yearly
due
to
various
factors.
Silver
nanoparticles
(AgNPs)
are
widely
utilized
for
their
low
cost
and
antibacterial
properties
compared
other
noble
metals.
Here,
AgNPs
were
synthesized
using
Cucurbita
maxima
leaf
extract,
which
was
analyzed
by
analytical
instruments.
UV-visible
spectra
showed
an
absorbance
peak
in
the
410-420
nm
range,
while
Fourier
transform
infrared
exhibited
bands
at
653
cm−1,
1629,
2276,
3786
cm−1.
X-ray
diffraction
major
intensity
peaks
38.1º
two
theta
value
with
average
crystallite
size
of
18.34
nm.
The
transmission
electron
microscope
aggregation
spherical-shaped
AgNPs,
10-90
MTT
assay
that
it
highly
effective
against
SiHa
cervical
cells
a
much
lower
IC50
(~8
µg/mL)
capable
pesticide
removal
highest
efficiency
pH
9.
Pharmaceuticals,
Год журнала:
2025,
Номер
18(5), С. 691 - 691
Опубликована: Май 7, 2025
Background:
Breast
cancer
and
chronic
bacterial
infections
are
pressing
global
health
issues,
traditional
treatments
often
hampered
by
resistance
adverse
side
effects.
This
study
sought
to
create
silver
nanoparticles
(AgNPs)
through
eco-friendly
synthesis
using
Hibiscus
rosa
sinensis
(HRS)
flower
extract
assess
their
antibacterial,
antibiofilm,
anticancer
properties.
Methods:
HRS
functioned
as
both
a
reducing
stabilizing
agent
in
the
of
AgNPs.
The
were
characterized
ultraviolet–visible
spectroscopy
(UV–Vis),
Fourier-transform
infrared
(FTIR)
spectroscopy,
transmission
electron
microscopy
(TEM).
Antibacterial
antibiofilm
properties
evaluated
against
gram-positive
(Staphylococcus
aureus
Enterococcus
faecalis)
gram-negative
(Escherichia
coli
Pseudomonas
aeruginosa)
bacteria
agar
well
diffusion
XTT
reduction
assays.
cytotoxic
effects
on
MDMB-231
breast
cells
normal
splenocytes
measured
MTT
assay,
whereas
fluorescence
was
used
observe
reactive
oxygen
species
(ROS)
production,
changes
mitochondrial
membrane
potential,
caspase-3
activation.
Results:
synthesized
HRS-AgNPs,
primarily
ranging
from
10
50
nm,
displayed
distinct
surface
plasmon
resonance
(SPR)
peak
at
428
nm.
They
exhibit
notable
antibacterial
activity,
especially
bacteria,
effectively
disrupt
biofilms.
Cytotoxicity
evaluations
showed
that
HRS-AgNPs
decreased
viability
dose-dependent
manner,
with
minimal
toxicity
observed
splenocytes.
increase
ROS
levels,
heightened
activity
collectively
suggest
apoptosis-driven
cell
death
cells.
Conclusions:
demonstrated
dual
functionality,
strong
selective
Their
environmentally
friendly
synthesis,
stability,
significant
biological
activities
potential
for
further
development,
including
vivo
safety
efficacy
assessments
clinical
applications
treating
cancer.
Green
synthesis
of
nanoparticles
has
become
a
significant
area
research,
driven
by
the
demand
for
sustainable,
economical,
and
environmentally
friendly
processes,
particularly
in
biomedical
applications.
In
this
study,
silver
(AgNPs)
were
produced
using
10
g
Couroupita
guianensis
flower
extract
through
straightforward
method.
The
biosynthesized
AgNPs
characterized
UV-Vis
spectroscopy,
XRD,
FTIR,
SEM,
EDS
techniques,
confirming
their
successful
formation
structural
properties.
antibacterial
efficacy
was
assessed
against
Escherichia
coli
Listeria
monocytogenes,
achieving
99.5%
inactivation
E.
99.3%
L.
monocytogenes
after
120
min
visible
light
exposure.
Additionally,
showed
notable
anticancer
effects
breast
cancer
cells
(MDA-MB-231),
displaying
dose-dependent
decrease
cell
viability,
with
reductions
15%,
30%,
72%,
86%
observed
at
concentrations
25,
50,
75,
100
μg/mL,
respectively.
spherical
morphology
synthesized
contributed
to
enhanced
interactions
bacterial
cells,
underpinning
effectiveness.
Furthermore,
mechanisms
underlying
these
analyzed,
highlighting
unique
phytochemical
properties
that
assist
biosynthesis
activity
enhancement.
These
findings
suggest
green-synthesized
derived
from
C.
hold
potential
use
applications,
therapies.
