Porcine parvovirus infection induces necroptosis of porcine placental trophoblast cells via a ZBP1-mediated pathway DOI Creative Commons
Ning Xu,

Qian Du,

Yijiao Cheng

и другие.

Veterinary Research, Год журнала: 2024, Номер 55(1)

Опубликована: Ноя. 29, 2024

Abstract Porcine parvovirus (PPV) infection induces germ cell death, leading to reproductive disorders in first-pregnant sows. placental trophoblast cells (PTCs) are the major target of PPV, and we have previously found that PPV leads death PTCs by a non-apoptotic process, which may be related pathogenicity. The Z-nucleic acid-binding protein 1 (ZBP1) is often activated after virus invasion mediates subsequent death. Here, induced ZBP1-mediated necroptosis porcine presence apoptosis inhibitor, AC-DEVD-CHO. ZBP1 expression was upregulated during infection, knockout or RNA interference significantly reduced its along with PPV-induced necroptosis. We discovered RIPK3 MLKL, but not Caspase-8, were involved downstream signaling infection; phosphorylation levels MLKL enhanced, Caspase-8 cleaved. infection-induced PTCs. did affect upregulation expression, blocked activation MLKL. infection. UV-inactivated less than non-irradiated PPV. A strain mutation translation initiation codon still able induce through ZBP1/RIPK3/MLKL pathway. These results provide new insights into pathogenic mechanism suggest viral DNA-dependent

Язык: Английский

CHMP4C promotes pancreatic cancer progression by inhibiting necroptosis via the RIPK1/RIPK3/MLKL pathway DOI Creative Commons

Longchen Yu,

Qining Guo,

Yaping Li

и другие.

Journal of Advanced Research, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

2
Adamantane-Quinoxalone Hybrids: Precision Chemotypes and Their Molecular Mechanisms in Acute Myeloid Leukemia DOI
Sangita Dattatray Shinde,

Ambika Chamoli,

Sai Swetha Uppalapati

и другие.

Journal of Medicinal Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Март 31, 2025

Acute myeloid leukemia (AML) is an aggressive blood cancer with a poor prognosis, especially when diagnosed late. Around 10–15% of cases involve the specific chromosomal abnormality t(8;21), which drives uncontrolled cell proliferation and contributes to disease onset. Despite advances in AML research treatment protocols, outcomes for t(8;21) remain stagnant, as patients receive standard, nonspecific chemotherapies. This one-size-fits-all approach targets both cancerous healthy cells, leading unwanted toxicity highlighting urgent need targeted therapies. In this study, we present precision chemotype based on quinoxalone-tethered adamantane framework developed via metal- light-free protocol. The compound selectively inhibits induces death by disrupting growth metabolic pathways, demonstrated through bioassays, RNA sequencing, proteomic analysis. Notably, it spares other leukemic solid underscoring its specificity potential therapy AML.

Язык: Английский

Процитировано

0
An insight into the therapeutic impact of quinoxaline derivatives: Recent advances in biological activities (2020–2024) DOI Creative Commons

Aly M. Waseem,

Ranya Mohammed Elmagzoub,

Mervat Mohammed Mazhar Abdelgadir

и другие.

Results in Chemistry, Год журнала: 2024, Номер unknown, С. 101989 - 101989

Опубликована: Дек. 1, 2024

Язык: Английский

Процитировано

3
Metal-free internal nucleophile-triggered domino route for synthesis of fused quinoxaline [1,4]-diazepine hybrids and the evaluation of their DNA binding properties DOI

Bhim Majhi,

Achyut Bora, Subhadeep Palit

и другие.

Bioorganic Chemistry, Год журнала: 2024, Номер 151, С. 107694 - 107694

Опубликована: Авг. 5, 2024

Язык: Английский

Процитировано

1
Porcine parvovirus infection induces necroptosis of porcine placental trophoblast cells via a ZBP1-mediated pathway DOI Creative Commons
Ning Xu,

Qian Du,

Yijiao Cheng

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Май 22, 2024

Abstract Porcine parvovirus (PPV) infection induces germ cell death, leading to reproductive disorders in first-pregnant sows. placental trophoblast cells (PTCs) are the major target of PPV, and we have previously found that PPV leads death PTCs by a non-apoptotic process, which may be related pathogenicity. The Z-nucleic acid-binding protein 1 (ZBP1) is often activated after virus invasion mediates subsequent death. Here, induced ZBP1-mediated necroptosis porcine presence apoptosis inhibitor, AC-DEVD-CHO. ZBP1 expression was upregulated during infection, knockout or RNA interference significantly reduced its along with PPV-induced necroptosis. We discovered RIPK3 MLKL, but not Caspase-8, were involved downstream signaling infection; phosphorylation levels MLKL enhanced, Caspase-8 cleaved. infection-induced PTCs. did affect upregulation expression, blocked activation MLKL. infection. UV-inactivated less than non-irradiated PPV. A strain mutation translation initiation codon still able induce through ZBP1/RIPK3/MLKL pathway. These results provide new insights into pathogenic mechanism suggest viral DNA-dependent

Язык: Английский

Процитировано

0
Porcine parvovirus infection induces necroptosis of porcine placental trophoblast cells via a ZBP1-mediated pathway DOI Creative Commons
Ning Xu,

Qian Du,

Yijiao Cheng

и другие.

Veterinary Research, Год журнала: 2024, Номер 55(1)

Опубликована: Ноя. 29, 2024

Abstract Porcine parvovirus (PPV) infection induces germ cell death, leading to reproductive disorders in first-pregnant sows. placental trophoblast cells (PTCs) are the major target of PPV, and we have previously found that PPV leads death PTCs by a non-apoptotic process, which may be related pathogenicity. The Z-nucleic acid-binding protein 1 (ZBP1) is often activated after virus invasion mediates subsequent death. Here, induced ZBP1-mediated necroptosis porcine presence apoptosis inhibitor, AC-DEVD-CHO. ZBP1 expression was upregulated during infection, knockout or RNA interference significantly reduced its along with PPV-induced necroptosis. We discovered RIPK3 MLKL, but not Caspase-8, were involved downstream signaling infection; phosphorylation levels MLKL enhanced, Caspase-8 cleaved. infection-induced PTCs. did affect upregulation expression, blocked activation MLKL. infection. UV-inactivated less than non-irradiated PPV. A strain mutation translation initiation codon still able induce through ZBP1/RIPK3/MLKL pathway. These results provide new insights into pathogenic mechanism suggest viral DNA-dependent

Язык: Английский

Процитировано

0