Piezo1-related physiological and pathological processes in glioblastoma

Frontiers in Cell and Developmental Biology, Год журнала: 2025, Номер 13

Опубликована: Фев. 21, 2025

Glioblastoma (GBM) is the most malignant of astrocytomas, primarily involving cerebral hemispheres and cortex. It one fatal refractory solid tumors with a 5-year survival rate only 5% in adults. Cells biological tissues are subjected to mechanical forces, including hydrostatic pressure, shear stress, compression tension. can convert mechanomechanical signals into or electrical signals, process known as signaling. Piezo1 channels, members Piezo family mechanosensitive ion be directly activated by stimuli alone, mediating cation currents that activate subsequent signaling pathways. Studies have shown largely unexpressed normal brain but expressed at high levels glioblastoma significantly contribute development progression, its role pathogenesis remains unclear. We reviewed relevant literature data six major databases PubMed, EMBASE, CINAHL, Scopus, Web Science TCGA. Finally, total 126 papers were selected for review analysis (Search terms include: glioblastoma, piezo1, biomechanical, targeted therapy, mechanomechanical, extracellular matrix, radiation therapy more). The piezo1 was summarized. affects several fundamental pathophysiological processes such tissue sclerosis, angiogenesis, energy supply, immune cell infiltration, used an indicator malignancy prognosis patients well therapeutic target control tumor progression. pathological mechanism very complex, aberrant expression plays important glioblastoma. Specific mechanistic studies focusing on will help us understand mechanobiology develop new approaches patients.

Язык: Английский

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Pharmacology of PIEZO1 channels

British Journal of Pharmacology, Год журнала: 2024, Номер 181(23), С. 4714 - 4732

Опубликована: Окт. 14, 2024

Abstract PIEZO1 is a eukaryotic membrane protein that assembles as trimers to form calcium‐permeable, non‐selective cation channels with exquisite capabilities for mechanical force sensing and transduction of into effect in diverse cell types include blood cells, endothelial epithelial fibroblasts stem cells systems bone, lymphatics muscle. The channel has wide‐ranging roles considered target novel therapeutics ailments spanning cancers cardiovascular, dental, gastrointestinal, hepatobiliary, infectious, musculoskeletal, nervous system, ocular, pregnancy, renal, respiratory urological disorders. identification modulators its infancy but useful experimental tools emerged activating, lesser extent inhibiting, the channels. Elementary structure–activity relationships are known Yoda series small molecule agonists, which show potential physicochemical pharmacological properties. Intriguing effects Yoda1 stimulated removal excess cerebrospinal fluid. Despite PIEZO1's broad expression, opportunities suggested selective positive or negative modulation without intolerable adverse effects. Here we provide focused, non‐systematic, narrative review progress this pharmacology discuss future directions research area.

Язык: Английский

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Wound Healing in Butterfly Pupal Wing Tissues: Real-Time In Vivo Imaging of Long-Range Cell Migration, Cluster Formation, and Calcium Oscillations

Insects, Год журнала: 2025, Номер 16(2), С. 124 - 124

Опубликована: Янв. 27, 2025

Insects can repair wounds and regenerate body parts in response to physical damage. Wound healing butterfly pupal wing tissues is developmentally interesting because ectopic color patterns develop during healing, suggesting that normal damage-induced may use similar mechanisms. Here we physiologically investigated wound pattern formation using the blue pansy Junonia orithya. In puncture damage, various are formed around damage site. After wounding operation, observed hemocytes migrating over long distances along veins (lacunae) toward site, where epidermal cells cellular clusters. Calcium oscillations were at near transiently affected by ruthenium red, an inhibitor of calcium transporters channels, red caused abnormalities scales adult wings. These results suggest cell migration, cluster formation, play important roles scale development Ectopic local as a consequence evolutionary co-option process for development.

Язык: Английский

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Charge‐Guided Cartilage‐Penetrating Micro/Nanocarriers That Inhibit Piezo1 and Protect Cartilage to Alleviate Osteoarthritis

Advanced Functional Materials, Год журнала: 2025, Номер unknown

Опубликована: Янв. 29, 2025

Abstract Osteoarthritis (OA), characterized by progressive cartilage degeneration and chronic joint pain, represents a major global health challenge. While intra‐articular drug delivery is common therapeutic approach, its efficacy limited poor penetration through the dense matrix, particularly for hydrophobic compounds. Here, novel charge‐guided micro/nanocarrier system designed to overcome these challenges presented. By modifying amylose with glycidyl trimethyl ammonium chloride (GTAC), it developed positively charged carriers that demonstrate enhanced into negatively matrix. The Piezo1 inhibitor, Margaric acid (MA), efficiently encapsulated within helical cavity of modified DMSO‐ultrasound treatment, creating Cationic Amylose Micro/Nanocarriers@MA (CAMNC@MA). This effectively delivers MA chondrocytes, where Piezo1, crucial mechanosensor implicated in OA development, can be precisely modulated. These results CAMNC@MA successfully inhibits abnormal mechanical force‐induced activation enhances cell survival, reduces matrix degradation, alleviates progression. innovative platform offers promising strategy clinical treatment combining efficient agents.

Язык: Английский

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Engineered endoplasmic reticulum-targeting nanodrugs with Piezo1 inhibition and promotion of cell uptake for subarachnoid hemorrhage inflammation repair

Journal of Nanobiotechnology, Год журнала: 2025, Номер 23(1)

Опубликована: Апрель 5, 2025

Subarachnoid hemorrhage (SAH) is a life-threatening acute hemorrhagic cerebrovascular condition, often presenting with severe headaches caused by intracranial hypertension, which in cases can lead to brain herniation. Piezo1 mechanosensitive ion channel protein whose mechanical properties are closely linked central nervous system diseases. In this study, we developed an engineered endoplasmic reticulum membrane-based nanomedicine (CAQKERM@GsMTx4) using HEK293T cells, aimed at targeted delivery regions, rapid absorption, and precise inhibition of therapy. To ensure optimal targeting therapeutic efficacy, fused the CAQK peptide gene N-terminus TRP-PK1, on membrane, loaded GsMTx4 into vesicles (EVs) derived from membrane. Through vivo vitro experiments multi-omics analysis, have demonstrated marked advantages membrane over cell vesicles. CAQKERM@GsMTx4 successfully inhibits SAH, helps microglia change M1 phenotype M2 phenotype, inflammatory responses neuronal damage. Overall, novel provides potential effective strategy for clinical treatment subarachnoid hemorrhage.

Язык: Английский

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Mitigating effects of hydroxysafflor yellow a on atherosclerotic inflammatory responses based on flavonoid macromolecule compound: Inhibition of Piezo1-YAP/JNK protein pathway

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 142961 - 142961

Опубликована: Апрель 1, 2025

Язык: Английский

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Opposing Calcium-Dependent Effects of GsMTx4 in Acute Lymphoblastic Leukemia: In Vitro Proliferation vs. In Vivo Survival Advantage

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(10), С. 4822 - 4822

Опубликована: Май 18, 2025

Mechanogated (MG) ion channels play a crucial role in mechano-transduction and immune cell regulation, yet their impact on blood cancers, particularly acute lymphoblastic leukemia (ALL), remains poorly understood. This study investigates the pharmacological effects of GsMTx4, an MG channel inhibitor, human ALL cells both vitro vivo. Unexpectedly, we found that GsMTx4 remarkably increased basal calcium (Ca2+) levels through constitutive Ca2+ entry enhanced store-operated Ca2⁺ influx upon thapsigargin stimulation. increase signaling promoted viability proliferation vitro. Notably, chelating intracellular with BAPTA-AM reduces GsMTx4-mediated proliferation. However, vivo, decreases cytosolic Nalm-6 GFP⁺ isolated from mouse blood, effectively countering progression significantly extending survival NSG mice transplanted (median survival: vs. control, 37.5 days 29 days, p = 0.0414). Our results highlight different properties activity vivo models. They also emphasize is key vulnerability leukemia, where its precise modulation dictates disease progression. Thus, targeting could offer novel therapeutic strategy for by exploiting homeostasis.

Язык: Английский

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A review on the role of Piezo1 in cardiovascular diseases: from molecular mechanisms to targeted therapeutic potential

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 144843 - 144843

Опубликована: Май 1, 2025

Язык: Английский

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Current status, hotspots and frontiers of ion channel-related research in glioblastoma: a bibliometric analysis from 2005 to 2024

Frontiers in Oncology, Год журнала: 2025, Номер 15

Опубликована: Июнь 4, 2025

Glioma is the most common primary tumor of central nervous system, with glioblastoma being its predominant pathological type. In recent years, research has demonstrated that ion channels are intricately linked numerous biological processes in cells, including apoptosis and migration, among others. This study employs bibliometric analysis to systematically examine synthesize knowledge structure focal points field channels. Publications related glioblastoma, published between January 1, 2005 December 31, 2024, were retrieved from Web Science Core Collection (WoSCC). The dataset comprises 764 articles 234 reviews. Utilize VOSviewer, CiteSpace, Bibliometrix, Excel conduct an in-depth visualization scholarly contributions based on country, institution, journal, author. Additionally, highly cited publications, references, keywords. From number publications this shown a consistent annual increase. University Alabama Cancer leading institutions journals. Sontheimer Harald, United States, prolific author field. co-cited references indicates background foundation focus elucidating mechanisms by which influence onset progression glioblastoma. Keyword indicated “tumor microenvironment” (burst strength: 4. 67), “Ca 2+ activated K + channel” 3. 98), “chloride channels” 59) have been keywords exhibiting highest burst intensity over past two decades. emerged higher frequency five years include “receptor” 11), “channels” 11). Research as prominent rapidly evolving interest. Previous studies primarily focused examination specific their functionalities. However, keyword highlights necessity explore interaction microenvironment. Meanwhile integrating single-cell spatial transcriptomics nanoparticle technologies, we can significantly enhance efficacy channel-targeting therapies.

Язык: Английский

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Piezo1 channel: A global bibliometric analysis from 2010 to 2024

Channels, Год журнала: 2024, Номер 18(1)

Опубликована: Сен. 16, 2024

In recent years, the Piezo1 channel has attracted great attention. Piezo1's research made remarkable advance in many aspects. However, overall trends and knowledge structures have not been systematically investigated from a worldwide viewpoint. Therefore, it is important to fill this gap utilize proper tool show status, hotspots, frontiers channel. order better investigate hotspots of research, we retrieved relevant literature Web Science Core Collection (WoSCC) applied CiteSpace perform bibliometric analysis. Our findings might serve as reference for future area.

Язык: Английский

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