Molecular Matchmakers: Bioconjugation Techniques Enhance Prodrug Potency for Immunotherapy DOI

Yinuo Chen,

Nicholas E. Clay,

Nathan Phan

и другие.

Molecular Pharmaceutics, Год журнала: 2024, Номер 22(1), С. 58 - 80

Опубликована: Ноя. 21, 2024

Cancer patients suffer greatly from the severe off-target side effects of small molecule drugs, chemotherapy, and radiotherapy─therapies that offer little protection following remission. Engineered immunotherapies─including cytokines, immune checkpoint blockade, monoclonal antibodies, CAR-T cells─provide better targeting future tumor growth prevention. Still, issues such as ineffective activation, immunogenicity, remain primary concerns. "Prodrug" therapies─classified therapies administered inactive then selectively activated to control time area release─hold significant promise in overcoming these Bioconjugation techniques (e.g., natural linker conjugation, bioorthogonal reactions, noncanonical amino acid incorporation) enable rapid homogeneous synthesis prodrugs selective loading immunotherapeutic agents carrier molecules protecting groups prevent after administration. Several prodrug activation mechanisms have been highlighted for cancer therapeutics, including endogenous by hypoxic or acidic conditions common tumors, exogenous targeted cleavage, stimuli-responsive light dual-stimuli which adds specificity combining mechanisms. This review will explore modern conjugation options, focusing on how strategies can enhance immunotherapy responses improve patient outcomes. We also discuss implications computational methodology therapy design recommend procedures determine where conjugate systems "prodrug" onto therapeutic safety delivery platforms.

Язык: Английский

Drug delivery for platinum therapeutics DOI
Hui Liu, Jiahui Zou, Xiaotong Li

и другие.

Journal of Controlled Release, Год журнала: 2025, Номер 380, С. 503 - 523

Опубликована: Фев. 11, 2025

Язык: Английский

Процитировано

0

Small Molecule Drugs Triggered the Activation of Macrocycle Masked Proteins DOI

Zhiying Zeng,

Wenkang Cai,

Yingze Liu

и другие.

Nano Letters, Год журнала: 2025, Номер unknown

Опубликована: Фев. 13, 2025

On-demand activation of prodrugs represents an emerging and fast developing strategy to improve the therapeutic index certain drugs. However, strategies generate protein-based with controllable are still limited. Here, we present a supramolecular masking that enables on-demand macrocycle-masked proteins Food Drug Administration (FDA)-approved oral Proteins interest were engineered incorporate two N-terminal peptide motifs, which dimerized by cucurbit[8]uril (CB[8]) form mask sterically blocks functional protein interfaces, inhibiting interactions targets or substrates. The inhibitory effect was selectively reversed amantadine memantine restore activity. This validated across various classes, including antibodies, cytokines, enzymes. Activation CB[8]-masked further demonstrated in living mice via FDA-approved small molecule treatments. Our method provided for selective development next-generation therapeutics.

Язык: Английский

Процитировано

0

Fundamental properties and principal areas of focus in antibody-drug conjugates formulation development DOI Creative Commons
Lei Wen, Yuanyuan Zhang, Chenxi Sun

и другие.

Antibody Therapeutics, Год журнала: 2025, Номер unknown

Опубликована: Март 9, 2025

Abstract Antibody–drug conjugates (ADCs) have emerged as a rapidly expanding class of therapeutics driven by their superior specificity and clinical efficacy. 14 out 16 commercially approved ADCs are formulated lyophilized forms because ADC is generally considered to be less stable than unmodified antibody. The formulation development for ADCs, particularly liquid formulation, presents unique challenges due intricate structural complexity, physicochemical properties, degradation pathways. This review provides the first comprehensive analysis strategies employed in commercial ADCs. Furthermore, this discusses key areas focus throughout workflow, spanning from initial final stage drug product manufacturing. In addition, we identify analyze distinctive technical compared unconjugated antibody, while proposing potential solutions these challenges. Finally, offer strategic perspectives on future directions advance promising therapeutic modality.

Язык: Английский

Процитировано

0

Comparison of conventional and green approaches to the synthesis of aromatic Schiff bases DOI Creative Commons
Beata Kołodziej

Polish Journal of Chemical Technology, Год журнала: 2024, Номер unknown

Опубликована: Окт. 1, 2024

Abstract The chemical industry is one of the key elements in improving quality human life. At same time, it generates pollution influencing ecosystem and our health. To limit or remove some reasons for two decades less harmful approaches to synthesis various organic compounds were developed. Among compounds, azomethines, also known as Schiff bases, are particular interest biochemistry, medicine, pharmacy. For over twenty years many novel these have been investigated. Toxic solvents (benzene, toluene) replaced with water, fruit juice, white egg, cases, temperature time process significantly reduced. In review, conventional green bases presented a focus on effectiveness methods, including advantages disadvantages.

Язык: Английский

Процитировано

2

Geldanamycins: Potent Hsp90 Inhibitors with Significant Potential in Cancer Therapy DOI Open Access
Omeima Abdullah, Ziad Omran

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(20), С. 11293 - 11293

Опубликована: Окт. 20, 2024

Geldanamycin, an ansa-macrolide composed of a rigid benzoquinone ring and aliphatic ansa-bridge, was isolated from Streptomyces hygroscopicus. Geldanamycin is potent heat shock protein inhibitor with remarkable antiproliferative activity. However, it shows pronounced hepatotoxicity in animal models unfavorable pharmacokinetic properties. Four geldanamycin analogs have progressed through various phases clinical trials, but none yet completed evaluation or received FDA approval. To enhance the efficacy these Hsp90 inhibitors, strategies such as prodrug approaches nanocarrier delivery systems could be employed to minimize systemic organ toxicity. Furthermore, exploring new drug combinations may help overcome resistance, potentially improving therapeutic outcomes. This review discusses mechanism action geldanamycin, its properties, alleviate toxicity maximize efficacy. The main focus on those derivatives that trials shown important vivo activity preclinical models.

Язык: Английский

Процитировано

1

Molecular Matchmakers: Bioconjugation Techniques Enhance Prodrug Potency for Immunotherapy DOI

Yinuo Chen,

Nicholas E. Clay,

Nathan Phan

и другие.

Molecular Pharmaceutics, Год журнала: 2024, Номер 22(1), С. 58 - 80

Опубликована: Ноя. 21, 2024

Cancer patients suffer greatly from the severe off-target side effects of small molecule drugs, chemotherapy, and radiotherapy─therapies that offer little protection following remission. Engineered immunotherapies─including cytokines, immune checkpoint blockade, monoclonal antibodies, CAR-T cells─provide better targeting future tumor growth prevention. Still, issues such as ineffective activation, immunogenicity, remain primary concerns. "Prodrug" therapies─classified therapies administered inactive then selectively activated to control time area release─hold significant promise in overcoming these Bioconjugation techniques (e.g., natural linker conjugation, bioorthogonal reactions, noncanonical amino acid incorporation) enable rapid homogeneous synthesis prodrugs selective loading immunotherapeutic agents carrier molecules protecting groups prevent after administration. Several prodrug activation mechanisms have been highlighted for cancer therapeutics, including endogenous by hypoxic or acidic conditions common tumors, exogenous targeted cleavage, stimuli-responsive light dual-stimuli which adds specificity combining mechanisms. This review will explore modern conjugation options, focusing on how strategies can enhance immunotherapy responses improve patient outcomes. We also discuss implications computational methodology therapy design recommend procedures determine where conjugate systems "prodrug" onto therapeutic safety delivery platforms.

Язык: Английский

Процитировано

0