Bioorganic Chemistry, Год журнала: 2025, Номер unknown, С. 108500 - 108500
Опубликована: Апрель 1, 2025
Язык: Английский
Current Issues in Molecular Biology, Год журнала: 2025, Номер 47(3), С. 155 - 155
Опубликована: Фев. 27, 2025
Objective: Non-small cell lung cancer (NSCLC) remains one of the most significant contributors to cancer-related mortality. This investigation explores influence and underlying mechanisms USP1 inhibitor SJB2-043 on A549 cells, with aim advancing development anti-NSCLC therapeutics. Methods: Publicly available databases were utilized assess expression its association progression NSCLC. Gene variations ascertained through RNA sequencing, followed by Kyoto Encyclopedia Genes Genomes Ontology pathway enrichment evaluations. Various doses administered cells evaluate impact multiplication, motility, apoptosis, cycle using CCK-8 assays, colony formation, wound healing, flow cytometry, Western blotting (WB). Results: was found be overexpressed in NSCLC specimens linked adverse prognosis. Treatment markedly inhibited proliferation migration, diminished clonogenic potential, triggered apoptosis a dose-dependent manner. Modifications observed, showing an elevated percentage G2 phase while exhibiting parallel decline G1 phase. WB examination demonstrated protein levels N-cadherin, CyclinB1, CDK1, C-myc, Bcl-2, p-ERK/ERK, p-p38/p38, p-JNK/JNK, p-AKT/AKT, p-mTOR/mTOR, alongside upregulation E-cadherin, ZO-1, occludin, p53, Bax, p-β-catenin/β-catenin, GSK3β. Conclusions: exerts suppressive effect proliferation, epithelial–mesenchymal transition enhancing apoptosis. These cellular effects appear mediated inhibition MAPK, Wnt/β-catenin, PI3K/AKT/mTOR signaling cascades, addition modulation cycle.
Язык: Английский
Процитировано
0
Frontiers in Oncology, Год журнала: 2025, Номер 15
Опубликована: Март 20, 2025
Background The ubiquitin proteasome system is involved in the regulation of cellular gene transcription and receptor function through degradation proteins, thus affecting tumorigenesis development. In this study, bioinformatics analysis revealed expression PSMD11 PSMD14 pancreatic ductal adenocarcinoma, which can be used as biomarkers for prognosis patients with PDAC. This study provides new targets prognostic assessment targeted therapy adenocarcinoma. Methods levels value adenocarcinoma were analyzed using GEPIA2, GEO, TCGA GTEx databases, relationships between these clinical case data survival analyzed. effects on malignant biological behaviors cancer cells, such proliferation, migration invasion, investigated by vitro experiments. Results Bioinformatics that mRNAs significantly higher (PDAC) tissues than normal high was correlated a poor Further evaluation correlation results characteristics PDAC associated lymph node metastasis, TNM grade, degree differentiation, Knockdown inhibited migration, invasion ability cells. Conclusion are highly expressed malignancy adenocarcinoma; thus, potential therapeutic patients.
Язык: Английский
Процитировано
0
Current Issues in Molecular Biology, Год журнала: 2025, Номер 47(3), С. 211 - 211
Опубликована: Март 20, 2025
USP5 has been proven to play an important role in the proliferation of bladder cancer (BC). In this study, we focused on investigating molecular mechanism ferroptosis induced by cancer. The was evaluated using T24 wild-type cells (WT) and knockout (USP5−/−) CCK8 colony formation assays. contents ferrobivalent ions (Fe2+), reactive oxygen species (ROS), malondialdehyde (MDA) were detected a determination kit observe relationship between ferroptosis. Furthermore, study employing Western blotting, co-immunoprecipitation, RT-qPCR, ubiquitination assays, etc. This showed genetic ablation significantly inhibited viability cells. Genetic promoted increases Fe2+ content, ROS, MDA levels. addition erastin increased USP5−/− their ROS contents. We verified that deficiency led significant reduction GPX4 protein levels overexpression could stabilize protein. Further studies interacts with stabilizes inhibiting its These findings revealed inhibits stabilizing GPX4. be potential therapeutic target for
Язык: Английский
Процитировано
0
Bioorganic Chemistry, Год журнала: 2025, Номер unknown, С. 108500 - 108500
Опубликована: Апрель 1, 2025
Язык: Английский
Процитировано
0