Scientific Reports,
Год журнала:
2025,
Номер
15(1)
Опубликована: Фев. 24, 2025
Malignant
melanoma,
a
rare
and
aggressive
skin
cancer,
arises
from
the
transformation
of
cutaneous
melanocytes
is
associated
with
poor
prognosis.
Evodiamine
(EVO),
bioactive
compound
derived
traditional
Chinese
herbal
medicine,
has
demonstrated
significant
inhibitory
effects
on
various
tumor
cells.
In
this
study,
we
aimed
to
investigate
potential
EVO
in
regulating
melanoma
immunity
elucidate
its
underlying
mechanisms.
Experimental
results
revealed
that
IC50
value
B16-F10
cells
for
24,
48,
72
h
were
11.73,
5.083,
4.604
µM,
respectively.
inhibited
proliferation,
invasion,
metastasis
by
more
than
50%,
while
promoting
apoptosis
higher
concentration
EVO.
also
significantly
suppressed
growth
80%
reduced
spleen
damage
tumor-bearing
mice.
Treatment
led
marked
increase
T-cell
subsets
spleen,
bone
marrow,
tumors,
immunohistochemical
(IHC)
staining
particular
showing
about
50%
higher.
Furthermore,
expression
programmed
cell
death
ligand
1
(PD-L1)
PI3K/AKT
signaling
pathway-related
proteins
both
tumors.
These
findings
suggest
exerts
antitumor
enhancing
immune
microenvironment
indicates
as
therapeutic
agent
melanoma.
International Journal of Biological Sciences,
Год журнала:
2024,
Номер
20(5), С. 1833 - 1854
Опубликована: Янв. 1, 2024
The
Warburg
Effect
is
one
of
the
most
well-known
cancer
hallmarks.This
metabolic
pattern
centered
on
lactate
has
extremely
complex
effects
various
aspects
tumor
microenvironment,
including
remodeling,
immune
suppression,
cell
migration,
and
drug
resistance
development.Based
accumulating
evidence,
metabolites
are
likely
to
participate
in
regulation
biological
processes
microenvironment
form
a
feedback
loop.Therefore,
further
revealing
key
mechanism
lactate-mediated
oncological
reasonable
scientific
idea.The
discovery
refinement
histone
lactylation
recent
years
laid
firm
foundation
for
above
idea.Histone
post-translational
modification
that
occurs
at
lysine
sites
histones.Specific
enzymes,
known
as
"writers"
"erasers",
catalyze
addition
or
removal,
respectively,
lactacyl
group
target
sites.An
increasing
number
investigations
have
reported
this
multiple
cellular
procedures.In
review,
we
discuss
close
connection
between
series
tumorigenesis,
infiltration,
energy
metabolism.Finally,
review
provides
insightful
perspectives,
identifying
promising
avenues
exploration
potential
clinical
application
field
research.
Signal Transduction and Targeted Therapy,
Год журнала:
2024,
Номер
9(1)
Опубликована: Сен. 16, 2024
Abstract
Histone
post-translational
modifications
(HPTMs),
as
one
of
the
core
mechanisms
epigenetic
regulation,
are
garnering
increasing
attention
due
to
their
close
association
with
onset
and
progression
diseases
potential
targeted
therapeutic
agents.
Advances
in
high-throughput
molecular
tools
abundance
bioinformatics
data
have
led
discovery
novel
HPTMs
which
similarly
affect
gene
expression,
metabolism,
chromatin
structure.
Furthermore,
a
growing
body
research
has
demonstrated
that
histone
also
play
crucial
roles
development
various
diseases,
including
cancers,
cardiovascular
infectious
psychiatric
disorders,
reproductive
system
diseases.
This
review
defines
nine
modifications:
lactylation,
citrullination,
crotonylation,
succinylation,
SUMOylation,
propionylation,
butyrylation,
2-hydroxyisobutyrylation,
2-hydroxybutyrylation.
It
comprehensively
introduces
modification
processes
these
HPTMs,
transcription,
replication,
DNA
repair
recombination,
structure,
well
involvement
promoting
occurrence
clinical
applications
targets
biomarkers.
Moreover,
this
provides
detailed
overview
HPTM
inhibitors
targeting
emerging
strategies
treatment
multiple
while
offering
insights
into
future
prospects
challenges.
Additionally,
we
briefly
introduce
techniques
field
research.
Frontiers in Genetics,
Год журнала:
2024,
Номер
15
Опубликована: Март 19, 2024
Background:
Prostate
cancer
(PCa)
is
one
of
the
most
common
malignancies
in
men
with
a
poor
prognosis.
It
therefore
great
clinical
importance
to
find
reliable
prognostic
indicators
for
PCa.
Many
studies
have
revealed
pivotal
role
protein
lactylation
tumor
development
and
progression.
This
research
aims
analyze
effect
lactylation-related
genes
on
PCa
Methods:
By
downloading
mRNA-Seq
data
TCGA
PCa,
we
obtained
differential
related
Five
machine
learning
algorithms
were
used
screen
key
then
five
overlapping
construct
survival
model
by
lasso
cox
regression
analysis.
Furthermore,
relationships
between
pathways,
mutation
immune
cell
subpopulations,
drug
sensitivity
explored.
Moreover,
two
risk
groups
established
according
score
calculated
(LRGs).
Subsequently,
nomogram
scoring
system
was
predict
disease-free
(DFS)
patients
combining
clinicopathological
features
scores.
In
addition,
mRNA
expression
levels
verified
lines
qPCR.
Results:
We
identified
5
LRGs
(ALDOA,
DDX39A,
H2AX,
KIF2C,
RACGAP1)
constructed
model.
The
AUC
values
1
-,
3
5-year
DFS
dataset
0.762,
0.745,
0.709,
respectively.
found
better
predictor
than
traditional
A
that
combined
variables
accurately
predicted
outcome
patients.
high-risk
group
higher
proportion
regulatory
T
cells
M2
macrophage,
burden,
worse
prognosis
those
low-risk
group.
had
lower
IC50
certain
chemotherapeutic
drugs,
such
as
Docetaxel,
Paclitaxel
be
highly
expressed
castration-resistant
cells.
Conclusion:
can
effectively
therapeutic
responses
Biological reviews/Biological reviews of the Cambridge Philosophical Society,
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 16, 2024
ABSTRACT
In
recent
years,
a
significant
breakthrough
has
emerged
in
biology,
the
identification
of
lactylation,
novel
post‐translational
process.
This
intriguing
modification
is
not
limited
to
specific
class
proteins
but
occurs
across
diverse
range,
including
histones,
signalling
molecules,
enzymes,
and
substrates.
It
can
exert
broad
regulatory
role
various
diseases,
ranging
from
developmental
anomalies
neurodegenerative
disorders
inflammation
cancer.
Thus,
it
presents
exciting
opportunities
for
exploring
innovative
treatment
approaches.
As
result,
there
been
surge
research
interest,
leading
deeper
understanding
molecular
mechanisms
functions
underlying
lactylation
within
physiological
pathological
processes.
Here,
we
review
detection
biological
disease
effects,
providing
systematic
overview
this
modification.
Frontiers in Immunology,
Год журнала:
2025,
Номер
15
Опубликована: Янв. 10, 2025
Lactate,
long
viewed
as
a
byproduct
of
glycolysis
and
metabolic
waste.
Initially
identified
within
the
context
yogurt
fermentation,
lactate's
role
extends
beyond
culinary
applications
to
its
significance
in
biochemical
processes.
Contemporary
research
reveals
that
lactate
functions
not
merely
terminal
product
but
also
nexus
for
initiating
physiological
pathological
responses
body.
Lysine
lactylation
(Kla),
novel
post-translational
modification
(PTM)
proteins,
has
emerged
pivotal
mechanism
by
which
exerts
regulatory
influence.
This
epigenetic
potential
alter
gene
expression
patterns,
thereby
impacting
Increasing
evidence
indicates
correlation
between
adverse
prognosis
various
malignancies.
Consequently,
this
review
article
aims
encapsulate
proteins
interact
with
lactate,
elucidate
tumorigenesis
progression,
explore
therapeutic
targets
afforded
modulation
lactylation.
The
objective
is
clarify
oncogenic
provide
strategic
framework
future
directions
burgeoning
field.
