Understanding and controlling the variables for advanced cell therapy
World Journal of Stem Cells,
Год журнала:
2024,
Номер
16(8), С. 784 - 798
Опубликована: Авг. 26, 2024
In
regenerative
medicine,
the
isolation
of
mesenchymal
stromal
cells
(MSCs)
from
adipose
tissue’s
vascular
fraction
(SVF)
is
a
critical
area
study.
Our
review
meticulously
examines
process
MSCs,
starting
with
extraction
tissue.
The
choice
liposuction
technique,
anatomical
site,
and
immediate
processing
are
essential
to
maintain
cell
functionality.
We
delve
into
intricacies
enzymatic
digestion,
emphasizing
fine-tuning
enzyme
concentrations
maximize
yield
while
preventing
harm.
then
outlines
filtration
centrifugation
techniques
necessary
for
isolating
purified
SVF,
alongside
viability
assessments
like
flow
cytometry,
which
vital
confirming
efficacy
isolated
MSCs.
discuss
advantages
drawbacks
using
autologous
vs
allogeneic
SVF
sources,
touching
upon
immunocompatibility
logistical
considerations,
as
well
variability
inherent
in
donor-derived
cells.
Anesthesia
choices,
selection
between
hypodermic
needles
cannulas,
role
tissue
lysers
achieving
cellular
dissociation
evaluated
their
impact
on
isolation.
Centrifugation
protocols
also
analyzed
part
ensuring
integrity
SVF.
necessity
standardized
MSC
highlighted,
promoting
reproducibility
successful
clinical
application.
encourage
ongoing
research
deepen
understanding
biology
therapeutic
action,
aiming
further
field
medicine.
concludes
call
rigorous
research,
interdisciplinary
collaboration,
strict
adherence
ethical
regulatory
standards
safeguard
patient
safety
optimize
treatment
outcomes
Язык: Английский
Enhancement of adipose stem cell quality via Cu‐MON: Transcriptome and bioinformatics analysis of normal and diabetic stem cells
Richong Pang,
K. Liu,
Biou Liu
и другие.
FASEB BioAdvances,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 18, 2025
Abstract
Transplanted
adipose
stem
cells
(ASC)
have
a
low
survival
rate
in
the
body,
and
there
are
not
many
ASC
that
can
be
effectively
used,
which
weakens
their
tissue
repair
function.
Based
on
this
status
quo,
new
type
of
copper‐based
metal–organic
network
(Cu‐MON)
was
used
to
pretreat
regulate
cell
activity
order
improve
efficacy
therapy
or
reduce
number
thus
reducing
cost
clinical
treatment.
Gene
expression
changes
before
after
Cu‐MON
treatment
normal
donor
(ND‐ASC)
2
diabetes
mellitus
(T2DM‐ASC)
were
evaluated
through
RNA
sequencing,
KEGG
GO
enrichment
analysis.
The
results
showed
improved
quality
by
regulating
immune
response
promoting
paracrine
secretion.
IL‐17
signaling
pathway
IL‐6,
CXCL8,
MMP‐9
key
pathways
necessary
genes
affected
ability
cells.
In
addition,
also
antiviral
Type
I
interferon
pathway.
Our
research
had
response,
secretion,
improving
capabilities.
This
approach
biomaterial
pretreatment
is
fast,
convenient,
relatively
safe,
provides
strategies
for
efficiency
therapies.
Язык: Английский
Clinical research progress of umbilical cord blood mesenchymal stem cells in Knee articular cartilage repair: A review
Medicine,
Год журнала:
2025,
Номер
104(6), С. e41402 - e41402
Опубликована: Фев. 7, 2025
Umbilical
cord
blood
mesenchymal
stem
cells
(UCB-MSCs)
are
a
type
of
adult
cell
with
multipotent
differentiation
potential
and
immunoregulatory
functions,
primarily
found
in
neonatal
blood.
Due
to
their
noninvasive
collection
method,
abundance,
ease
preservation,
UCB-MSCs
represent
promising
biological
material.
This
review
examines
the
clinical
research
on
knee
articular
cartilage
repair,
highlighting
regenerative
for
treating
joint
defects.
Our
aim
is
provide
insights
into
current
applications
propose
directions
future
research,
focusing
optimizing
use
enhancing
patient
outcomes.
Язык: Английский
miRNA let-7f-5p-encapsulated labial gland MSC-derived EVs ameliorate experimental Sjögren’s syndrome by suppressing Th17 cells via targeting RORC/IL-17A signaling axis
Journal of Nanobiotechnology,
Год журнала:
2025,
Номер
23(1)
Опубликована: Март 20, 2025
Sjögren's
syndrome
(SS)
is
an
autoimmune
disease
primarily
affecting
salivary
glands,
with
xerostomia
as
a
distinct
clinical
manifestation.
This
also
poses
significantly
increased
risk
of
lymphoma,
severely
impacting
patients'
quality
life.
The
imbalance
between
Th17
and
Treg
cells
plays
critical
role
in
SS
progression,
driving
severe
immune
dysregulation,
chronic
inflammation,
escalating
tissue
dysfunction.
However,
current
treatments
for
still
remain
limited,
it
continues
to
be
recognized
refractory
disease.
Therefore,
the
development
novel
effective
therapeutic
strategies
pressing
demand
research.
In
recent
years,
extracellular
vesicle
(EV)
therapy
has
emerged
promising
approach
treatment,
showing
encouraging
outcomes
modulating
balance
alleviating
symptoms.
EVs
carry
diverse
cargo,
among
which
microRNAs
(miRNAs)
are
highly
abundant
play
roles.
These
small
RNAs
essential
EV-mediated
functions,
particularly
regulating
gene
expression
microenvironment.
Our
research
team
first
isolated
labial
gland
mesenchymal
stem
(LGMSCs)
their
derived
(LGMSC-EVs),
offer
potential
advantages
due
origin.
Then
we
screened
identified
enriched
miRNA
let-7f-5p
key
regulator
through
profiling
analysis.
To
achieve
better
outcomes,
transfected
exogenous
into
LGMSC-EVs
upregulate
its
expression,
thereby
constructing
let-7f-5p-encapsulated
LGMSC-EVs.
modified
were
subsequently
tested
experimental
mouse
model
evaluate
potential.
upregulation
enhanced
effects,
resulting
improvements
such
flow
reduced
lymphocytic
infiltration.
Mechanistically,
suppressed
by
directly
targeting
3'-untranslated
region
(3'UTR)
RORC,
inhibiting
RORC/IL-17A
signaling
axis,
reducing
IL-17A
production,
restoring
Th17/Treg
promoting
anti-inflammatory
profile.
Collectively,
this
LGMSC-EV
offers
target-driven
treatment
SS,
achieving
improved
rebalance
after
modification
let-7f-5p,
presents
new
SS.
Язык: Английский
Cell membrane-coated nanoparticles: a biomimetic strategy for effective treatment of ischemic stroke
Journal of Drug Delivery Science and Technology,
Год журнала:
2025,
Номер
unknown, С. 106966 - 106966
Опубликована: Апрель 1, 2025
Язык: Английский
Comparative breakthrough: Umbilical cord mesenchymal stem cells vs bone marrow mesenchymal stem cells in heart failure treatment
World Journal of Cardiology,
Год журнала:
2024,
Номер
16(12), С. 776 - 780
Опубликована: Ноя. 25, 2024
In
this
article,
we
evaluate
the
comparative
efficacy
and
safety
of
mesenchymal
stem
cells
(MSCs)
derived
from
bone
marrow
(BM-MSCs)
umbilical
cord
(UC-MSCs)
in
treatment
heart
failure
myocardial
infarction.
MSCs
have
gained
importance
as
living
bio
drug
due
to
their
regenerative
potential,
with
BM-MSCs
being
most
extensively
studied.
However,
UC-MSCs
offer
unique
advantages,
such
noninvasive
collection
fewer
ethical
concerns.
This
systematic
review
meta-analysis
summarizes
data
13
randomized
controlled
trials,
which
included
a
total
693
patients.
Their
study
shows
that
significantly
improved
left
ventricular
ejection
fraction
by
5.08%
at
6
months
2.78%
12
compared
controls,
while
showed
no
significant
effect.
Neither
cell
type
changes
6-minute
walk
distance.
addition,
had
comparable
profiles,
differences
major
adverse
cardiac
events,
except
for
lower
rehospitalization
rate
observed
BM-MSCs.
These
results
position
promising
alternative
MSC-based
therapies
disease,
offering
potential
improvements
function
maintaining
favorable
profile.
Future
research
should
focus
on
optimizing
administration
protocols
further
exploring
long-term
benefits
mechanisms
repair.
Язык: Английский
Dental Pulp-Derived Mesenchymal Stromal Cells
Springer eBooks,
Год журнала:
2024,
Номер
unknown, С. 85 - 126
Опубликована: Янв. 1, 2024
Язык: Английский
Cell Secretome from Mesenchymal Stem Cells for Periodontal Regeneration
Regenerative medicine and dentistry.,
Год журнала:
2024,
Номер
unknown, С. 4 - 4
Опубликована: Дек. 23, 2024
Review
Cell
Secretome
from
Mesenchymal
Stem
Cells
for
Periodontal
Regeneration
Bin
Chen
1,†,
Lili
Li
Xiaoxin
Zhang
2,
Qing
Liu
1,
Yin
Xiao
3,*
and
Fuhua
Yan
1,*
1
Department
of
Periodontology,
Nanjing
Stomatological
Hospital,
Affiliated
Hospital
Medical
School,
Institute
Stomatology,
University,
210008,
China
2
Central
Laboratory
3
School
Medicine
Dentistry
&
Biomedicine
Glycomics,
Griffith
Gold
Coast,
QLD
4222,
Australia
*
Correspondence:
[email protected]
(Y.X.);
[email protected]
(F.Y.)
†
These
authors
contributed
equally
to
this
work.
Received:
6
November
2024;
Revised:
12
December
Accepted:
16
Published:
23
2024
Abstract:
Periodontitis
affects
approximately
50%
the
global
adult
population
results
in
varying
degrees
periodontal
destruction.
The
regeneration
tissue
is
great
demand
but
currently
difficult
achieve.
cell
secretome
mesenchymal
stem
cells
(CSmsc)
has
shown
promise
promoting
a
translational
alternative
(MSC)-based
therapy.
practical
components
CSmsc
are
soluble
secretions
extracellular
vesicles.
mechanisms
CSmsc-induced
may
lie
its
regulation
local
microenvironment,
modulating
immune
such
as
macrophages
stimulating
host
MSCs,
with
direct
effect
on
regeneration.
Therefore,
been
suggested
promising
cell-based
product
future
regenerative
Hence,
more
studies
should
be
conducted
analyse
effective
MSC
secretome,
explore
underlying
mechanisms,
obtain
functional
clinical
translation
Язык: Английский