Calcium speciation and coordination environment in intracellular amorphous calcium carbonate (ACC) formed by cyanobacteria
Chemical Geology,
Год журнала:
2023,
Номер
641, С. 121765 - 121765
Опубликована: Окт. 5, 2023
Язык: Английский
Diel changes in the expression of a marker gene and candidate genes for intracellular amorphous CaCO3 biomineralization in Microcystis
Peer Community Journal,
Год журнала:
2025,
Номер
5
Опубликована: Фев. 6, 2025
Язык: Английский
Diel changes in the expression of a marker gene and candidate genes for intracellular amorphous CaCO3biomineralization inMicrocystis
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Июль 7, 2024
Phylogenetically
diverse
cyanobacteria
biomineralize
intracellular
amorphous
calcium
carbonate
(iACC)
inclusions.
This
includes
several
genotypes
of
the
Microcystis
genus,
a
potentially
toxic,
bloom-forming
cyanobacterium
found
worldwide
in
freshwater
ecosystems.
While
we
ignore
biological
function
iACC
and
molecular
mechanisms
driving
their
formation,
this
process
may
impact
local
geochemical
cycles
and/or
be
used
for
bioremediation
strategies.
Recently,
marker
gene
biomineralization
pathway,
named
ccyA,
was
discovered.
However,
calcyanin
protein
encoded
by
ccyA
remains
unknown.
Here,
based
on
an
RNA-Seq
approach,
assess
expression
aeruginosa
PCC
7806
during
24
h
day/night
cycle.
The
shows
clear
pattern
with
maximum
transcript
abundances
second
half
night.
is
consistent
assumption
that
related
photosynthesis
therefore
follow
cycle
as
well.
Moreover,
genes
directly
co-localized
upstream
downstream
same
DNA
strand
show
similar
pattern,
including
cax
encoding
calcium/proton
exchanger
domain
also
present
N-terminal
region
calcyanins
many
iACC-forming
cyanobacteria.
suggests
they
all
could
part
operon,
play
concerted
role
formation.
Last,
other
involved
carbon
concentrating
transport
to
ccyA.
Overall,
study
provides
list
candidate
whose
be,
future,
analyzed
biochemistry
genetics
approaches.
Язык: Английский
Calcium isotope fractionation by intracellular amorphous calcium carbonate (ACC) forming cyanobacteria
Geobiology,
Год журнала:
2024,
Номер
22(2)
Опубликована: Март 1, 2024
Abstract
The
formation
of
intracellular
amorphous
calcium
carbonate
(ACC)
by
various
cyanobacteria
is
a
widespread
biomineralization
process,
yet
its
mechanism
and
importance
in
past
modern
environments
remain
to
be
fully
comprehended.
This
study
explores
whether
(Ca)
isotope
fractionation,
linked
ACC‐forming
cyanobacteria,
can
serve
as
reliable
tracer
for
detecting
these
microorganisms
ancient
settings.
Accordingly,
we
measured
stable
Ca
fractionation
during
uptake
the
cyanobacterium
Cyanothece
sp.
PCC
7425.
Our
results
show
that
7425
cells
are
enriched
lighter
isotopes
relative
solution.
finding
consistent
with
kinetic
effects
observed
biogenic
marine
calcifying
organisms.
composition
was
accurately
modeled
using
Rayleigh
model,
resulting
factor
(Δ
44
Ca)
equal
−0.72
±
0.05‰.
Numerical
modeling
suggests
primarily
unidirectional,
minimal
back
reaction
over
duration
experiment.
Finally,
compared
our
Δ
values
those
other
biotic
abiotic
carbonates,
revealing
similarities
organisms
form
calcite.
These
raise
questions
about
effectiveness
univocal
environment.
We
propose
use
combination
proposed
tracers
such
Ba
Sr
factors
and/or
elevated
Ba/Ca
Sr/Ca
ratios
may
provide
more
approach.
Язык: Английский