Sustainable Refinery Waste Management through Biotechnological Interventions: Health Impacts, Historical Successes, and Emerging Solutions

Environmental Research, Год журнала: 2025, Номер unknown, С. 120967 - 120967

Опубликована: Янв. 1, 2025

Язык: Английский

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Elucidating the synergistic role of hybrid peptide from Burkholderia cepacia enzymes in biodegradation of polycyclic aromatic hydrocarbons

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Апрель 12, 2025

Polycyclic aromatic hydrocarbons (PAHs) are hazardous environmental contaminants emerging from industrial activities and fossil fuel combustion, posing risks to human health ecosystems. Biodegradation offers a sustainable approach mitigating PAH pollution, here we investigated the efficacy of peptide hybrid laccase O-methyltransferase enzymes bacterium Burkholderia cepacia in degradation. Both demonstrated stability with an instability index below 40, indicating suitability for application. Following active site prediction, 3D structure hybrid, consisting 71 amino acids, was modelled using trRosetta, achieving high-quality ERRAT score above 97%. Further bioinformatic analysis confirmed hybrid's non-allergenic non-virulent properties. Molecular docking studies revealed robust binding affinity - 9 kcal/mol, highlighting this potential effective degradation suggesting its promise as eco-friendly bioremediation agent PAH-contaminated sites.

Язык: Английский

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Natural Hydrocarbon-Contaminated Springs as a Reservoir of Microorganisms Useful for Bioremediation: Isolation and Multilevel Analysis of Hydrocarbonoclastic Bacteria from the Agri Valley (Southern Italy)

Sustainability, Год журнала: 2025, Номер 17(7), С. 3083 - 3083

Опубликована: Март 31, 2025

This research aimed to characterise hydrocarbonoclastic bacteria isolated from naturally hydrocarbon-contaminated springs and the surrounding soils in Agri Valley (Southern Italy) assess effectiveness of bioaugmentation using a four-strain microbial consortium for removing hydrocarbons artificially diesel-contaminated lake waters mesocosm experiments. Four novel bacterial strains were selected experimentation: Gordonia amicalis S2S5, Rhodococcus erythropolis S2W2, Acinetobacter tibetensis S2S8, puyangensis S1W1. The four isolates can use diesel oil as their sole carbon source, some exhibited relatively high emulsifying capacity ability adhere hydrocarbons. Furthermore, genome analyses revealed presence genes associated with degradation, detoxification, transport various contaminants. Mesocosm experiments demonstrated that enhanced capacities native communities remove hydrocarbons, although drastic changes composition (analysed through Next-Generation Sequencing—NGS) observed. Taken together, these results suggest contaminated environments serve valuable reservoir microorganisms significant biotechnological potential, particularly field bioremediation. However, complete understanding efficiently degrade contaminants requires further fully capabilities limitations across different settings.

Язык: Английский

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Advances in the Degradation of Polycyclic Aromatic Hydrocarbons by Yeasts: A Review

Microorganisms, Год журнала: 2024, Номер 12(12), С. 2484 - 2484

Опубликована: Дек. 2, 2024

Polycyclic aromatic hydrocarbons (PAHs) are toxic organic compounds produced during the incomplete combustion of materials and commonly found in environment due to anthropogenic activities such as industrial vehicular emissions well natural sources, mainly volcanic eruptions forest fires. PAHs known for their bioaccumulative capacity environmental persistence, raising concerns adverse effects on human health, including carcinogenic potential. In recent years, bioremediation has emerged a promising, effective, sustainable solution degradation contaminated environments. this context, yeasts have proven be key microorganisms these compounds, owing ability metabolize them through series enzymatic pathways. This review explores advancements yeast-mediated PAHs, with particular focus role enzymes cytochrome P450 (CYPs), epoxide hydrolases (EHs), glutathione S-transferases (GSTs), which facilitate breakdown compounds. The also discusses applications genetic engineering enhance efficiency PAH use omics technologies predict catabolic potential organisms. Additionally, it examines studies addressing benzo[a]pyrene (BaP) by Debaryomyces hansenii, future implications sciences developing new bioremediation.

Язык: Английский

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Thriving in salty environments: Aspergillus niger’s halotolerance and BTEX biodegradation potential

World Journal of Microbiology and Biotechnology, Год журнала: 2024, Номер 41(1)

Опубликована: Дек. 18, 2024

Язык: Английский

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