Triphenyl Phosphate Alters Methyltransferase Expression and Induces Genome-Wide Aberrant DNA Methylation in Zebrafish Larvae DOI Creative Commons
Chander K. Negi,

Lucie Bláhová,

Audrey Phan

и другие.

Chemical Research in Toxicology, Год журнала: 2024, Номер 37(9), С. 1549 - 1561

Опубликована: Авг. 29, 2024

Emerging environmental contaminants, organophosphate flame retardants (OPFRs), pose significant threats to ecosystems and human health. Despite numerous studies reporting the toxic effects of OPFRs, research on their epigenetic alterations remains limited. In this study, we investigated exposure 2-ethylhexyl diphenyl phosphate (EHDPP), tricresyl (TMPP), triphenyl (TPHP) DNA methylation patterns during zebrafish embryonic development. We assessed general toxicity morphological changes, measured global hydroxymethylation levels, evaluated methyltransferase (DNMT) enzyme activity, as well mRNA expression DNMTs ten-eleven translocation (TET) methylcytosine dioxygenase genes. Additionally, analyzed genome-wide in larvae using reduced-representation bisulfite sequencing. Our assessment revealed no toxicity, but a statistically yet subtle decrease body length following TMPP EHDPP, along with reduction head height after TPHP exposure, was observed. Eye diameter width were unaffected by any OPFRs. There changes levels group, showed clear effect DNMT expression. However, EHDPP significantly decreased only DNMT1 expression, while reduced several orthologues TETs larvae, leading aberrant methylation. Differential occurred primarily introns (43%) intergenic regions (37%), 9% 10% occurring exons promoter regions, respectively. Pathway enrichment analysis differentially methylated region-associated genes indicated that enhanced biological molecular functions corresponding metabolism neurological KEGG further TPHP-mediated potential signaling pathways including TGFβ, cytokine, insulin signaling. This study identifies specific brings novel insights into mode action TPHP.

Язык: Английский

Revealing the neurodevelopmental toxicity of face mask-derived microplastics to humans based on neural organoids DOI
Minghui Li, Xue Gao,

Yingying Lan

и другие.

Journal of Hazardous Materials, Год журнала: 2025, Номер unknown, С. 138084 - 138084

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0
Health Risks of Low-Dose Dietary Exposure to Triphenyl Phosphate and Diphenyl Phosphate in Mice: Insights from the Gut–Liver Axis DOI

Jing Cao,

Xinwei Wang, Yumeng Lei

и другие.

Environmental Science & Technology, Год журнала: 2025, Номер unknown

Опубликована: Апрель 29, 2025

Aryl phosphate esters have been detected throughout the natural environment and in human blood samples, making it important to determine health risks associated with exposure triphenyl (TPHP) its metabolite diphenyl (DPHP). Here, C57BL/6J male mice were exposed TPHP or DPHP for 12 weeks at estimated daily intake doses of 0.1 7 μg/kg bw/day. affected levels short-chain fatty acids bile gut, enhancing production 29 medium- long-chain liver by 3.72-fold significantly increasing hepatic lipid cholesterol levels. Metabolomic molecular analysis confirmed that elevated persisted after an 8 week recovery period. Gut microbiota-dependent alterations toxic end points observed TPHP-fed mice, as supported results fecal microbiota transplantation. In DPHP-fed serotonergic glutamatergic synapses simultaneously altered intestine, corresponding reduction five brain neurotransmitters (15.4-60.8%). Decreased carbohydrate insulin resistance mice. These suggest affect metabolism via different modes, mediated through gut-liver axis, providing novel insights into mechanisms organophosphate-ester-mediated metabolic disruption.

Язык: Английский

Процитировано

0
Triphenyl phosphate interferes with the synthesis of steroid hormones through the PPARγ/CD36 pathway in human trophoblast JEG‐3 cells DOI
Yuting Chen, Qian Liu, Yao Wang

и другие.

Environmental Toxicology, Год журнала: 2024, Номер 39(6), С. 3400 - 3409

Опубликована: Март 7, 2024

Abstract Triphenyl phosphate (TPhP), a chemical commonly found in human placenta and breast milk, has been shown to disturb the endocrine system. Our previous study confirmed that TPhP could accumulate interference with placental lipid metabolism steroid hormone synthesis, as well induce endoplasmic reticulum (ER) stress through PPARγ trophoblast JEG‐3 cells. However, molecular mechanism underlying this disruption remains unknown. aimed identify role of PPARγ/CD36 pathway TPhP‐induced disruption. We increased accumulation, total cholesterol, low‐ high‐density protein progesterone, estradiol, glucocorticoid, aldosterone levels, genes related hormones including 3βHSD1 , 17βHSD1 CYP11A CYP19 CYP21 . These effects were largely blocked by co‐exposure either antagonist GW9662 or knockdown CD36 using siRNA (siCD36). Furthermore, an ER inhibitor 4‐PBA attenuated effect on progesterone glucocorticoid siCD36 reduced stress‐related levels induced TPhP, BiP, PERK, CHOP. findings suggest may also play synthesis TPhP. As our shed light pathway's involvement disturbance biosynthesis cells, further investigations potential impacts function following birth outcome are warranted.

Язык: Английский

Процитировано

2
Occupational exposure to traditional and emerging organophosphate esters: A comparison of levels across different sources and blood distribution DOI Creative Commons

Jingren Cui,

Yanhui Ge,

Meiqi Guo

и другие.

Environment International, Год журнала: 2024, Номер 194, С. 109165 - 109165

Опубликована: Ноя. 22, 2024

Язык: Английский

Процитировано

2
Ecological and human health risk of aryl-phosphate flame retardants (APFRs): Sources, distribution, and toxicity DOI
Yuanxiang Jin,

Juntao Wang,

Haigui Xu

и другие.

Critical Reviews in Environmental Science and Technology, Год журнала: 2023, Номер 54(15), С. 1117 - 1137

Опубликована: Дек. 20, 2023

Aryl-phosphate flame retardants (APFRs), a subcategory of organophosphate (OPFRs), are present in diverse range materials including plastic, and foam products, furniture, electronic waste. Despite their widespread use, research on the toxicity APFRs remains limited. In order to clarify more clearly environmental health risks caused by APFRs, this review provides comprehensive overview occurrence four APFRs: triphenyl phosphate (TPHP), 2-ethylhexyl diphenyl (EHDPP), isobutyl (IDPP), (DPHP). Regarding it seems that EHDPP TPHP act different ways intestine, but both cause disorders lipid metabolism liver. On contrary, toxic effects IDPP liver may be similar, specific mechanisms require further study. At same time, DPHP, as main biotransformation should receive attention for its toxicity. Prioritizing investigation vivo transformation particularly DPHP enhancing study APFRs' biodegradation crucial comprehensively assess APFRs.

Язык: Английский

Процитировано

4
Reference gene considerations for toxicological assessment of the flame retardant triphenyl phosphate in an in vitro fish embryonic model DOI Creative Commons

Logan Germain,

Delaine Pereira,

Louise M. Winn

и другие.

Journal of Applied Toxicology, Год журнала: 2024, Номер unknown

Опубликована: Сен. 18, 2024

Abstract The reliability of relative quantification RT‐qPCR depends upon the gene interest being normalized to one or more reference genes, with assumption that chosen genes do not experience altered expression experimental conditions. correct choice stable is critical when investigating alterations transcript levels following exposure endocrine and metabolic disrupting chemicals, such as flame retardant triphenyl phosphate (TPhP). This study assessed stability eight TPhP in embryonic cells derived from rainbow trout ( Oncorhynchus mykiss ). β‐actin actb ) 18s rRNA were stable, while glyceraldehyde‐3‐phosphate dehydrogenase gapdh) was found be increased. gapdh a popular has been previously used literature for teleost fish models. We discuss implications upregulation context chemical. Furthermore, we quantified tumor suppressor p53 relation different use an example report on how discrepancies findings might arise depending gene.

Язык: Английский

Процитировано

1
Cresyl diphenyl phosphate (a novel organophosphate ester) induces hepatic steatosis by directly binding to liver X receptor α: From molecule action to risk assessment DOI Creative Commons
Xinya Liu,

Lanchao Sun,

Yongfeng Lin

и другие.

Environment International, Год журнала: 2024, Номер 194, С. 109168 - 109168

Опубликована: Ноя. 26, 2024

Язык: Английский

Процитировано

1
Upregulation of oxidative stress by triphenyl phosphate (TPhP) exposure causes antioxidant insult and apoptotic process in Epithelioma papulosum cyprini (EPC) cells DOI

Ning‐Xia Xiong,

Zi‐Xuan Fang,

Xu‐Ying Kuang

и другие.

Environmental Science and Pollution Research, Год журнала: 2023, Номер 30(56), С. 119217 - 119227

Опубликована: Ноя. 3, 2023

Язык: Английский

Процитировано

3
Mechanism of interaction between ascorbic acid and soil iron-containing minerals for peroxydisulfate activation and organophosphorus flame retardant degradation DOI

Xiaona Dong,

Miaomiao Dai, Tongyi Yang

и другие.

Environmental Research, Год журнала: 2023, Номер 244, С. 117883 - 117883

Опубликована: Дек. 9, 2023

Язык: Английский

Процитировано

3
4-Phenylbutyric Acid Treatment Reduces Low-Molecular-Weight Proteinuria in a Clcn5 Knock-in Mouse Model for Dent Disease-1 DOI Open Access
Ana Perdomo-Ramírez, Elena Ramos‐Trujillo, José David Machado

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(15), С. 8110 - 8110

Опубликована: Июль 25, 2024

Dent disease-1 (DD-1) is a rare X-linked tubular disorder characterized by low-molecular-weight proteinuria (LMWP), hypercalciuria, nephrolithiasis and nephrocalcinosis. This disease caused inactivating mutations in the

Язык: Английский

Процитировано

0