Ecotoxicology and Environmental Safety,
Год журнала:
2024,
Номер
287, С. 117268 - 117268
Опубликована: Ноя. 1, 2024
Per-
and
polyfluoroalkyl
substances
(PFAS)
have
attracted
significant
attention
due
to
their
environmental
toxicity.
However,
the
detrimental
impact
of
PFAS
on
development
female
reproductive
system
remains
controversial.
In
this
study,
we
investigated
effects
three
specific
compounds
perfluorooctanoic
acid
(PFOA),
perfluorononanoic
(PFNA),
perfluorodecanoic
(PFDA)
ovarian
development.
Among
these
compounds,
PFDA
demonstrated
most
pronounced
cytotoxic
effect
granulosa
cells.
The
results
showed
that
a
200
μM
concentration
induced
cell
apoptosis
via
intrinsic
pathway
by
elevating
reactive
oxygen
species
(ROS)
levels
activating
Caspase-9
Caspase-3.
Furthermore,
triggered
necroptosis,
form
regulated
death
(RCD),
through
receptor-interacting
serine/threonine
kinase
1
(RIPK1),
receptor
interacting
protein
3
(RIPK3),
mixed-lineage
domain-like
(MLKL)
axis,
mediated
inhibition
canonical
proteolytic
enzyme
Caspase-8.
vivo
experiments
confirmed
mice
exposed
displayed
significantly
reduced
index
compared
control
group,
accompanied
evident
follicular
atresia.
Ovarian
tissues
from
PFDA-exposed
group
upregulated
necroptosis
markers,
which
were
effectively
mitigated
inhibiting
phosphorylation
RIPK1
at
Ser166.
Importantly,
study
provides
first
evidence
disrupts
novel
mechanism
involving
RIPK1-mediated
pathway,
alongside
detection
pathway.
This
greatly
expands
our
insight
into
death.
finding
highlights
potential
public
health
hazards
associated
with
exposure
emphasizes
need
for
further
research
fully
understand
its
broader
implications.
Environmental Pollution,
Год журнала:
2025,
Номер
372, С. 125968 - 125968
Опубликована: Март 4, 2025
Per-
and
polyfluoroalkyl
substances
(PFAS)
are
synthetic
chemicals
that
persist
in
the
environment,
bioaccumulate,
may
have
toxic
effects.
This
study
used
non-native
cane
toads
(Rhinella
marina)
to
examine
PFAS
metal
accumulation
impacts
large
terrestrial
amphibians
from
urban
peri-urban
areas.
We
quantified
38
compounds
36
environmental
legacy
metal(loid)s
52
adult
toad
livers
collected
six
locations
around
Southeast
Queensland,
Australia,
along
a
known
gradient.
Associations
among
PFAS,
metal(loid)
concentrations,
whole-organism
metrics
were
assessed.
An
omics-led
approach
assessed
biochemical
responses
liver,
muscle,
fat
gonad/egg
tissues
associated
with
these
concentrations.
Liver
concentrations
ranged
0.5
82.1
μg/kg
ww,
one
male
outlier
at
452
ww
(mean:
18
±
21
SD
excluding
outlier).
PFOS
was
most
dominant
(60
26
SD%
of
total),
followed
by
PFDoDA
(13
9
SD%).
The
liver
statistically
significant
variation
sex
Al,
As,
Ca,
Cu,
Mn,
Ni,
Se,
Sn,
Sr
V.
Total
had
no
associations
metrics,
body
condition
relative
femur
length
showed
weak
interaction
effect
between
Ni.
Metabolic
profiling
revealed
sex-specific
differences
linked
total
females
showing
broader
metabolic
perturbation.
strongest
signals
glycerolipid
metabolism,
ether
lipid
fatty
acid
biosynthesis,
though
effects
weak.
levels
low
compared
those
previously
recorded
tertiary
consumers
aquatic
vertebrates
contaminated
Despite
minor
metabolomic
changes,
overall
health
impact
minimal.
These
findings
contribute
development
tissue
guideline
values
for
wild
amphibians,
but
further
studies
on
higher
additional
amphibian
species
needed.
Environment International,
Год журнала:
2024,
Номер
190, С. 108864 - 108864
Опубликована: Июль 2, 2024
Perfluorinated
alkyl
substances
(PFAS)
are
pervasive
environmental
contaminants
that
have
attracted
considerable
attention
due
to
their
widespread
utilization,
resilient
characteristics,
adverse
health
implications,
and
regulatory
scrutiny.
Despite
documented
toxicity
in
living
organisms,
the
precise
molecular
mechanisms
governing
induced
effects
remain
unclear.
This
study
aims
elucidate
of
toxic
action
by
collecting
empirical
data
sets
along
oxidative
stress
metabolic
disruption
pathways.
We
investigated
impact
long-chain
PFAS
(perfluorooctanoic
acid
(PFOA))
its
short-chain
analog
(perfluorobutanoic
(PFBA))
on
human
neuronal
cells
(SH-SY5Y).
The
functionalities
enzymes
associated
with
(catalase
glutathione
reductase)
cellular
metabolism
(lactate
dehydrogenase
pyruvate
dehydrogenase)
were
also
characterized.
Our
results
reveal
a
24-hour
exposure
PFOA
PFBA
generated
significant
levels
reactive
oxygen
species.
Correspondingly,
there
was
notable
decline
catalase
reductase
activities,
demonstrating
more
pronounced
effect.
High
concentrations
reduced
activity.
Lactate
activity
only
impacted
high
concentration
PFBA,
while
decreased
increased
exposure.
findings
from
this
contribute
knowledge
cell
interactions
potential
underlying
PFAS-induced
toxicity.
Ecotoxicology and Environmental Safety,
Год журнала:
2024,
Номер
278, С. 116402 - 116402
Опубликована: Май 9, 2024
Perfluorobutanesulfonic
acid
(PFBS),
a
short-chain
alternative
to
perfluorooctanesulfonic
(PFOS),
is
widely
used
in
various
products
and
increasingly
present
environmental
media
human
bodies.
Recent
epidemiological
findings
have
raised
concerns
about
its
potential
adverse
health
effects,
although
the
specific
toxic
mechanism
remains
unclear.
This
study
aimed
investigate
metabolic
toxicity
of
gestational
PFBS
exposure
maternal
rats.
Pregnant
Sprague
Dawley
(SD)
rats
were
randomly
assigned
three
groups
administered
either
3%
starch
gel
(control),
5,
or
50
mg/kg
bw·d
PFBS.
Oral
glucose
tolerance
tests
(OGTT)
lipid
profiles
measured,
integrated
omics
analysis
(transcriptomics
non-targeted
metabolomics)
was
employed
identify
changes
genes
metabolites
their
relationships
with
phenotypes.
The
results
revealed
that
exposed
exhibited
significant
decrease
1-h
levels
area
under
curve
(AUC)
OGTT
compared
group.
Transcriptomics
indicated
alterations
gene
expression
related
cytochrome
P450
exogenous
metabolism,
glutathione
bile
secretion,
tumor
pathways,
retinol
metabolism.
Differentially
expressed
(DEMs)
enriched
pathways
such
as
pyruvate
glucagon
signaling
pathway,
central
carbon
metabolism
cancer,
citric
cycle.
Co-enrichment
pairwise
correlation
among
genes,
metabolites,
outcomes
identified
several
differentially
(DEGs),
including
Gstm1,
Kit,
Adcy1,
Gck,
Ppp1r3c,
Ppp1r3d,
DEMs
fumaric
acid,
L-lactic
4-hydroxynonenal,
acetylvalerenolic
acid.
These
DEGs
may
play
role
modulation
glucolipid
pathways.
In
conclusion,
our
suggest
induce
molecular
perturbations
homeostasis.
provide
insights
into
mechanisms
contributing
heightened
risk
abnormal
associated
exposure.
Ecotoxicology and Environmental Safety,
Год журнала:
2024,
Номер
287, С. 117260 - 117260
Опубликована: Ноя. 1, 2024
Studies
have
linked
per-
and
polyfluoroalkyl
substances
(PFAS)
to
chronic
metabolic
diseases.
However,
the
relationship
between
PFAS
exposure
insulin
resistance
(IR),
a
key
pathophysiological
basis
of
these
diseases,
in
nondiabetic
individuals
yet
be
determined.
Environment International,
Год журнала:
2024,
Номер
187, С. 108716 - 108716
Опубликована: Май 1, 2024
Benzotriazoles
(BTRs)
are
a
class
of
benzoheterocyclic
chemicals
that
frequently
used
as
metal-corrosive
inhibitors,
both
in
industry
and
daily
use.
However,
the
exposure
effect
information
on
BTRs
remains
relatively
limited.
In
this
study,
an
integrated
metabolomic
transcriptomic
approach
was
utilized
to
evaluate
three
BTRs,
benzotriazole,
6-chloro-1-hydroxi-benzotriazole,
1-hydroxy-benzotriazole,
mouse
liver
with
results
showing
disrupted
basal
metabolic
processes
vitamin
cofactor
metabolism
after
28
days.
The
expression
several
genes
related
inflammatory
response
aryl
hydrocarbon
receptor
pathways,
such
Gstt2
Arntl,
altered
by
BTRs.
Exposure
also
affected
metabolites
involved
immune
system
xenobiotic
responses.
cytochrome
P450
family
reveal
potential
detoxification
mechanism
liver.
Taken
together,
our
findings
provide
new
insights
into
multilayer
well
resource
for
further
exploration
molecular
mechanisms
which
occurs.
Metabolites,
Год журнала:
2024,
Номер
14(7), С. 392 - 392
Опубликована: Июль 19, 2024
Per-
and
polyfluoroalkyl
substances
(PFAS)
represent
a
class
of
persistent
synthetic
chemicals
extensively
utilized
across
industrial
consumer
sectors,
raising
substantial
environmental
human
health
concerns.
Epidemiological
investigations
have
robustly
linked
PFAS
exposure
to
spectrum
adverse
outcomes.
Altered
metabolites
stand
as
promising
biomarkers,
offering
insights
into
the
identification
specific
pollutants
their
deleterious
impacts
on
health.
However,
elucidating
metabolic
alterations
attributable
ensuing
effects
has
remained
challenging.
In
light
this,
this
review
aims
elucidate
potential
biomarkers
by
presenting
comprehensive
overview
recent
metabolomics-based
studies
exploring
toxicity.
Details
types,
sources,
patterns
are
provided.
Furthermore,
PFAS-induced
liver
toxicity,
reproductive
developmental
cardiovascular
glucose
homeostasis
disruption,
kidney
carcinogenesis
synthesized.
Additionally,
thorough
examination
utilizing
metabolomics
delineate
toxicity
blood,
liver,
urine
specimens
is
presented.
This
endeavors
advance
our
understanding
regarding
associated
toxicological
effects.