Application of organoid technology in the human health risk assessment of microplastics: A review of progresses and challenges

Environment International, Год журнала: 2024, Номер 188, С. 108744 - 108744

Опубликована: Май 11, 2024

Microplastic (MP) pollution has become a global environmental issue, and increasing concern been raised about its impact on human health. Current studies the toxic effects mechanisms of MPs have mostly conducted in animal models or vitro cell cultures, which limitations regarding inter-species differences stimulation cellular functions. Organoid technology derived from pluripotent adult stem cells broader prospects for predicting potential health risks to humans. Herein, we reviewed current application advancements opportunities different organoids, including brain, retinal, intestinal, liver, lung assess MPs. techniques accurately simulate complex processes reflect phenotypes related diseases caused by such as liver fibrosis, neurodegeneration, impaired intestinal barrier cardiac hypertrophy. Future perspectives were also proposed technological innovation risk assessment using extending lifespan organoids chronic toxicity MPs, reconstructing multi-organ interactions explore their studying microbiome-gut-brain axis effect

Язык: Английский

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Integrated approaches for immunotoxicity risk assessment: challenges and future directions

Deleted Journal, Год журнала: 2024, Номер 1(1)

Опубликована: Окт. 29, 2024

The integration of immunotoxicity data into chemical risk assessment paradigms is essential for comprehensively evaluating the potential health hazards posed by exposures. This review provides a comprehensive overview methodologies, challenges, and future directions integrating frameworks. It discusses fundamental principles immunotoxicology its relevance to assessment, highlighting critical roles immune system in defense against harmful agents. Next, we explore traditional frameworks, including exposure hazard identification, dose–response characterization, need incorporating endpoints enhance characterization estimation. Subsequently, delve modelling immunotoxicity, elucidating principles, methods, case studies illustrating relationships extrapolation from animal humans. Furthermore, discuss focusing on identification immunotoxic hazards, evaluation endpoints, utilization immunological biomarkers assessment. We then examine cumulative strategies, presenting conceptual framework assessing risks multiple exposures methods different chemicals. Lastly, emerging trends high-throughput screening assays, omics technologies, computational modelling, alternative testing along with regulatory implications research needs. valuable insights researchers, regulators, stakeholders involved public protection, facilitating development evidence-based strategies safeguarding human associated

Язык: Английский

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Human kidney organoids for modeling the development of different diseases

Current topics in developmental biology/Current Topics in Developmental Biology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Язык: Английский

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The impact of emerging contaminants exposure on human health effects: A review of organoid assessment models

Chemical Engineering Journal, Год журнала: 2024, Номер 498, С. 155882 - 155882

Опубликована: Сен. 16, 2024

Язык: Английский

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Unveiling the Heart’s Hidden Enemy: Dynamic Insights into Polystyrene Nanoplastic-Induced Cardiotoxicity Based on Cardiac Organoid-on-a-Chip

ACS Nano, Год журнала: 2024, Номер 18(45), С. 31569 - 31585

Опубликована: Ноя. 1, 2024

Exposure to micro- and nanoplastics (MNPs) has been implicated in potential cardiotoxicity. However, vitro models based on cardiomyocyte cell lines lack crucial cardiac characteristics, while interspecies differences animal compromise the reliability of conclusions. In addition, current research predominantly focused single-time point exposures MNPs, neglecting comparative analyses injury across early late stages. Moreover, there remains a large gap understanding susceptibility MNPs under pathological conditions. To address these limitations, this study integrated organoids (COs) organ-on-a-chip (OoC) technology develop organoid-on-a-chip (COoC), which was validated for cardiotoxicity evaluation through multiple dimensions. Based COoC, we conducted dynamic observation damage caused by short- long-term exposure polystyrene (PS-NPs). Oxidative stress, inflammation, disruption calcium ion homeostasis, mitochondrial dysfunction were confirmed as mechanisms PS-NP-induced events stages, fibrosis emerged prominent feature Notably, low-dose exacerbated myocardial infarction symptoms states, despite no significant shown healthy models. conclusion, findings further deepened our cardiotoxic effects introduced promising platform assessing

Язык: Английский

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Organoid models: applications and research advances in colorectal cancer

Frontiers in Oncology, Год журнала: 2025, Номер 15

Опубликована: Фев. 7, 2025

This review summarizes the applications and research progress of organoid models in colorectal cancer research. First, high incidence mortality rates are introduced, emphasizing importance organoids as a model. Second, this provides detailed introduction to concept, biological properties, organoids, including their strengths mimicking structural functional aspects organs. article further analyzes adult stem cell-derived pluripotent discusses advancements for basic research, drug development, personalized treatment evaluation prediction, regenerative medicine. Finally, prospects applying technology its significant value improving patient survival rates. In conclusion, systematically explains highlighting tremendous potential promising

Язык: Английский

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Advances in organoid-on-a-chip for recapitulation of human physiological events

Materials Today, Год журнала: 2025, Номер unknown

Опубликована: Фев. 1, 2025

Язык: Английский

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Absorption of Toxicants from the Ocular Surface: Potential Applications in Toxicology

Biomedicines, Год журнала: 2025, Номер 13(3), С. 645 - 645

Опубликована: Март 6, 2025

In relation to the eye, body can absorb substances from ocular surface fluid (OSF) in a few ways: directly through conjunctival sac, nasal mucosa as drains into nose, or ingestion. Regardless of absorption method, sac should be used toxicological matrix, even though only small quantities are needed. Contemporary analytical techniques make it suitable matrix for research. Analyzing and nano-quantities analyte requires high-cost, sophisticated tools, which is particularly relevant high-throughput environment new drug cosmetics testing. Environmental toxicology also presents challenge, many pollutants enter system using same route. A review existing literature was conducted assess potential applications clinical forensic related toxicants surface. The selection studies this aimed identify new, more efficient, cost-effective technology diagnostic methods.

Язык: Английский

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Microfluidic organoid-slice-on-a-chip system for studying anti-cholangiocarcinoma drug efficacy and hepatorenal toxicity

Lab on a Chip, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Organ-chip technology, in contrast to cell culture and animal models, offers a promising platform for accelerating drug development. However, current chip designs simulate human organ functions there is lack of multi-organ that can simultaneously study efficacy hepatorenal toxicity. Here, we developed novel microfluidic integrated cholangiocarcinoma organoids (CCOs) with recellularized liver slices (RLS) kidney (RKS), assess anti-cholangiocarcinoma Co-culture patient-derived CCOs RLS RKS was successfully achieved 7 days under flow conditions enhanced renal functions. Furthermore, an vitro biomimetic model showed IC50 values trastuzumab emtansine (T-DM1) around 6.42 ± 7.34 μg mL-1 four clinical cases, one outlier 77.77 due patient variability. Post-treatment, viability remained high at 75.67% 81.03%, respectively, suggesting low toxicity T-DM1 treating cholangiocarcinoma. Our demonstrates the use organoid-slice-on-a-chip (OSOC) personalized assessment, particularly aiming leveraging anticancer drugs off-label save lives.

Язык: Английский

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Metabolic Dysfunction-Associated Steatotic Liver Disease Induced by Microplastics: An Endpoint in the Liver–Eye Axis

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(7), С. 2837 - 2837

Опубликована: Март 21, 2025

There is a significant, rather than just anecdotal, connection between the liver and eyes. This evident in noticeable cases such as jaundice, where sclera has yellow tint. But this can be seen through even more subtle indicators, molecules known hepatokines. relationship not merely anecdotal; some studies, it referred to “liver–eye axis”. Ubiquitous environmental contaminants, microplastics (MPs), enter bloodstream human body conjunctival sac, nasolacrimal duct, upper respiratory tract mucosa. Once absorbed, these substances accumulate various organs cause harm. Toxic from surface of eye lead local oxidative damage by inducing apoptosis corneal cells, irregularly shaped microparticles exacerbate effect. Even other toxicants ocular may absorbed into distributed throughout body. Environmental toxicology presents challenge because many pollutants same route that used certain medications. Previous research indicated accumulation MPs play major role development chronic disease humans. It crucial investigate whether buildup potential fibrosis, or simply consequence conditions cirrhosis portal hypertension.

Язык: Английский

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