Oligotrophs
are
predominant
in
nutrient-poor
environments,
but
copiotrophic
bacteria
may
tolerate
conditions
of
low
energy
and
can
also
survive
thrive
these
nutrient-limited
conditions.
In
the
present
study,
we
isolated
648
strains
using
a
dilution
plating
method
after
enrichment
for
low-nutrient
We
collected
150
seawater
samples
at
21
stations
different
parts
water
column
Zhenbei
Seamount
South
China
Sea.
The
that
could
grow
on
medium
were
genera
42
species.
A
total
99.4%
(644/648)
phylum
Pseudomonadota,
with
73.3%
(472/644)
class
Gammaproteobacteria
26.7%
(172/644)
Alphaproteobacteria.
Among
representative
isolates,
Pseudoalteromonas
arabiensis,
Roseibium
aggregatum,
Vibrio
neocaledonicus
all
layers
almost
stations.
Almost
half
species
(20/42)
contained
genes
performed
nitrate
reduction,
confirmation
by
reduction
testing.
These
isolates
functioned
sulfur
metabolism,
including
sulfate
thiosulfate
oxidation,
disproportionation,
dimethylsulfoniopropionate
degradation.
GH23,
CBM50,
GT4,
GT2,
GT51
main
carbohydrate-active
enzymes
(CAZymes),
five
or
strains.
most
abundant
classes
CAZymes
those
associated
degradation
chitin,
starch,
cellulose.
Collectively,
our
study
marine
capable
growing
demonstrated
diversity
their
potential
metabolic
characteristics.
Environmental Microbiology Reports,
Год журнала:
2025,
Номер
17(2)
Опубликована: Апрель 1, 2025
ABSTRACT
Despite
extensive
research
on
microbiota
across
land
use
gradients,
it
remains
unclear
if
microbial
co‐occurrence
relationships
exhibit
consistent
patterns.
Here,
we
assessed
networks
of
seven
natural
ecosystems—
Quercus
ilex
forest,
Fagus
sylvatica
Abies
alba
Mediterranean
and
mountain
grasslands,
subalpine
shrublands—and
five
agroecosystems,
including
vineyards,
horticulture,
greenhouse,
a
polluted
agricultural
system,
an
arid
greenhouse.
Soil
chemistry,
such
as
pH,
organic
carbon
total
nitrogen,
was
characterised,
soil
were
profiled
using
high‐throughput
sequencing
from
242
samples.
Our
results
revealed
that
grasslands
had
the
highest
(86.4
g/kg),
while
greenhouse
lowest
(6.1
g/kg).
pH
5.79,
vineyards
electrical
conductivity
0.901
dS/m.
Notably,
ecosystem
exhibited
greater
modularity,
with
protected
horticulture
showing
exceptionally
(0.937),
intensive
agriculture
within
agroecosystems
significantly
lower
modularity
0.282.
Modularity
number
modules
positively
correlated
P
2
O
5
,
network
diameter,
path
length
clustering
coefficient
pH.
Additionally,
edges
nodes
number,
average
degree
diversity
associated
nitrogen.
These
findings
highlight
ecosystems
foster
more
complex
resilient
networks,
underscoring
sustainable
management's
importance
to
preserve
health
diversity.
Global Change Biology,
Год журнала:
2024,
Номер
30(9)
Опубликована: Сен. 1, 2024
Abstract
Subsoil
is
a
large
organic
carbon
reservoir,
storing
more
than
half
of
the
total
soil
(SOC)
globally.
Conventionally,
subsoil
assumed
to
not
be
susceptible
climate
change,
but
recent
studies
document
that
change
could
significantly
alter
cycling.
However,
little
known
about
microbial
responses
interactive
effects
warming
and
altered
precipitation.
Here,
we
investigated
cycling
associated
in
both
(30–40
cm)
topsoil
(0–10
Tibetan
alpine
grassland
over
4
years
Compared
unchanged
(
β
=
.55,
p
.587),
exhibited
stronger
response
effect
precipitation
2.04,
.021),
is,
decreased
content
by
28.20%
under
while
increased
18.02%
precipitation.Furthermore,
512
metagenome‐assembled
genomes
(MAGs)
were
recovered,
including
representatives
phyla
with
poor
genomic
representation.
only
one
changed
MAG,
16
MAGs
affected
precipitation,
5
More
twice
as
many
populations
whose
MAG
abundances
correlated
variations
content,
components
fluxes
observed
topsoil,
suggesting
their
potential
contribution
mediating
Collectively,
our
findings
highlight
sensitive
specific
lineages
provide
key
information
for
predicting
future
scenarios.
ABSTRACT
Soil
depth
determines
microbial
community
composition.
Yet,
it
remains
largely
unexplored
how
climate
changes
affect
the
vertical
distribution
of
soil
communities.
Here,
we
investigated
effects
altered
precipitation
and
nighttime
warming
on
communities
in
topsoils
(0–20
cm)
subsoils
(20–50
a
temperate
grassland
Inner
Mongolia,
China.
As
commonly
observed
under
nutrient
scarcity
conditions,
bacterial
fungal
α-diversity
network
complexity
decreased
with
depth.
However,
protistan
increased,
which
was
attributed
to
less
niche
overlap
smaller
body
size.
Strikingly,
slopes
linear
regressions
α-diversity/network
were
all
reduced
by
precipitation.
Microbial
composition
significantly
influenced
both
precipitation,
lesser
extent
elevated
The
ribosomal
RNA
gene
operon
(
rrn
)
copy
number,
genomic
proxy
demand,
depth,
percentages
positive
links
higher
subsoil,
supporting
“hunger
game”
hypothesis.
Both
number
while
increasing
links,
enhancing
potential
sharing
among
species.
stochasticity
level
assemblies
showing
that
acted
as
selection
force.
Altered
increased
stochasticity,
attenuating
depth’s
filtering
effect
diminishing
its
relationship
diversity.
Collectively,
unveiled
predominant
influence
affecting
IMPORTANCE
Understanding
change
impacts
is
critical
for
predicting
ecosystem
responses
global
environmental
shifts.
exhibit
strong
depth-related
stratification,
yet
variables,
such
warming,
these
patterns
have
been
inadequately
studied.
Our
research
uncovers
disrupts
previously
relationships
between
diversity,
finding
challenges
traditional
models
ecology.
Furthermore,
our
study
provides
experimental
support
hunger
game
hypothesis,
highlighting
oligotrophic
microbes,
characterized
lower
numbers,
are
selectively
favored
nutrient-poor
subsoils,
fostering
cooperation
resource
exchange.
By
unraveling
complexities
communities,
findings
offer
crucial
insights
developing
strategies
mitigate
adverse
impacts.
