Comparative characterization of organ-specific phase I and II biotransformation enzyme kinetics in salmonid S9 sub-cellular fractions and cell lines
Cell Biology and Toxicology,
Год журнала:
2025,
Номер
41(1)
Опубликована: Янв. 28, 2025
Язык: Английский
Mechanism of Hydrogen Production through Lignin Supercritical Water Gasification Catalyzed by Carbon-supported Nickel-based Catalyst: ReaxFF Molecular Dynamics Simulation
Energy,
Год журнала:
2025,
Номер
unknown, С. 135036 - 135036
Опубликована: Фев. 1, 2025
Язык: Английский
Xenometabolomics in Ecotoxicology: Concepts and Applications
Environmental Science & Technology,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 22, 2025
Nontargeted
high-resolution
mass
spectrometry
(HRMS)
allows
for
the
characterization
of
a
large
fraction
exposome,
i.e.,
entirety
chemicals
an
organism
is
exposed
to,
and
helps
detect
important
exogenous
chemical
compounds
that
could
be
key
drivers
toxicological
impact.
Along
with
these
occur
endogenous
metabolites
are
essential
health
host
organism.
Chemical
derived
from
biotransformation
xenobiotics
present
in
exposome
referred
to
as
xenometabolome,
while
endometabolome.
Recent
advancements
HRMS
technology
allow
detection
features
biological
ecological
importance
context
safety
assessments
unprecedented
sensitivity
resolution.
In
this
perspective,
we
highlight
application
HRMS-based
metabolomics
organisms
ecotoxicology,
complexity
comprehensively
characterizing
endometabolome,
distinguishing
xenometabolome.
Язык: Английский
Xenometabolome of Early-Life Stage Salmonids Exposed to 6PPD-Quinone
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Июнь 14, 2024
Abstract
N
-(1,3-Dimethylbutyl)-
N′
-phenyl-
p
-phenylenediamine-quinone
(6PPD-Q)
is
a
ubiquitous
transformation
product
(TP)
derived
from
the
rubber
tire
antioxidant
-phenylenediamine
(6PPD)
and
acutely
toxic
to
certain
species
of
Salmonidae
.
Not
all
salmonids
are
sensitive
acute
lethality
caused
by
6PPD-Q,
with
6PPD-Q
potency
varying
several
orders
magnitude
among
teleosts.
The
main
driver(s)
sensitivity
differences
(are)
pressing
question,
one
area
interest
examining
whether
in
teleosts
ability
biotransform
detoxify
could
be
key
factor.
This
study
utilized
liquid-chromatography
high-resolution
mass
spectrometry
(LC-HRMS)
assess
biotransformation
metabolome-wide
effects
on
early-life
stage
salmonids,
including
two
species,
rainbow
trout
(
Oncorhynchus
mykiss
)
lake
Salvelinus
namaycush
),
tolerant
brown
Salmo
trutta
).
Three
phase
I
TPs
seven
II
were
detected,
peak
areas
revealing
that
had
greatest
6PPD-Q.
Monohydroxylated
verified
using
co-developed
analytical
standards
will
use
for
future
biomonitoring
exposure
assessment.
Several
endogenous
metabolites
found
dysregulated
trout,
indicative
mitochondrial
dysfunction,
altered
metabolism,
disrupted
membrane
permeability.
Results
this
indicate
difference
capability
fish
subsequent
unique
metabolome
responses.
Graphical
Synopsis
Inter-species
salmonid
leads
variability
Язык: Английский