Frontiers in Microbiology,
Год журнала:
2022,
Номер
12
Опубликована: Фев. 22, 2022
The
contamination
of
antibiotic
resistance
genes
(ARGs)
may
directly
threaten
human
health.
This
study
used
a
metagenomic
approach
to
investigate
the
ARG
profile
in
drinking
water
treatment
system
(DWTS)
south
China.
In
total,
317
subtypes
were
detected;
specifically,
encoding
bacitracin,
multidrug,
and
sulfonamide
widely
detected
DWTS.
Putative
hosts
included
Acidovorax
(6.0%),
Polynucleobacter
(4.3%),
Pseudomonas
(3.4%),
Escherichia
(1.7%),
Klebsiella
(1.5%)
as
enriched
biomarkers
DWTS,
which
mainly
carried
beta-lactam,
aminoglycoside
ARGs.
From
further
analysis
ARG-carrying
contigs
(ACCs),
Stenotrophomonas
maltophilia
aeruginosa
most
common
pathogens
among
49
ACC
binning
results
demonstrated
that
33
high-quality
metagenome-assembled
genomes
(MAGs)
discovered
DWTS;
particularly,
MAG
identified
S.
maltophilia-like
(bin.195)
harbored
greatest
number
(n
=
8),
namely,
multidrug
6;
smeD,
semE,
multidrug_transporter,
mexE,
semB,
smeC),
beta-lactam
1;
metallo-beta-lactamase),
[n
aph(3')-IIb].
strong
positive
correlation
between
MGEs
revealed
high
dissemination
risk
Based
on
pure-culture
method,
93
isolates
belong
30
genera
recovered
from
Specifically,
multidrug-resistant
opportunistic
such
P.
aeruginosa,
Bacillus
cereus,
These
insights
into
DWTS's
resistome
indicated
need
for
more
comprehensive
monitoring
management
Furthermore,
effective
disinfection
methods
be
developed
remove
ARGs
DWTSs,
these
findings
could
assist
governing
bodies
surveillance
DWTSs.
Ecotoxicology and Environmental Safety,
Год журнала:
2021,
Номер
220, С. 112317 - 112317
Опубликована: Май 25, 2021
There
are
rising
concerns
about
microbes
harboring
antibiotic
resistance
genes
(ARGs)
and
virulence-associated
(VAGs)
in
humans
food-producing
animals.
Moreover,
ARGs
considered
as
emerging
environmental
pollutants,
posing
probable
life-threatening
complications
Commensal
Escherichia
coli
(E.
coli)
strain
can
carry
a
large
number
of
VAGs,
which
may
become
opportunistic
pathogen.
The
objective
this
study
was
to
determine
the
prevalence
possible
association
VAGs
E.
isolates
from
clinically
healthy
waterfowls
China's
tropical
island,
Hainan.
For
purpose,
311
non-repeating
were
evaluated
for
phenotypic
drug
linked
with
ARGs.
Additionally,
strains
examined
subsequent
virulence
by
uniplex
or
multiplex
PCR
sequencing.
Overall,
89
types
patterns
analysed,
while
25
23
observed,
qnrS
(99.4%)
iucD
(99.7%)
most
commonly
found
genes,
respectively.
Significant
positive
associations
observed
among
(p<0.05,
OR>1).
strongest
between
gene
iss
(OR,
76.25;
95%
CI,
4.02–1445.42).
Our
results
propose
that
serve
reservoir
carrying
multi
various
ExPEC
associated
VAGs.
Therefore,
provides
necessary
information
on
occurrence
waterfowls,
act
reference
regulatory
use
antibiotics
stop
direct
indirect
spread
these
resistant
potential
virulent
natural
environment.
Frontiers in Microbiology,
Год журнала:
2022,
Номер
12
Опубликована: Фев. 22, 2022
The
contamination
of
antibiotic
resistance
genes
(ARGs)
may
directly
threaten
human
health.
This
study
used
a
metagenomic
approach
to
investigate
the
ARG
profile
in
drinking
water
treatment
system
(DWTS)
south
China.
In
total,
317
subtypes
were
detected;
specifically,
encoding
bacitracin,
multidrug,
and
sulfonamide
widely
detected
DWTS.
Putative
hosts
included
Acidovorax
(6.0%),
Polynucleobacter
(4.3%),
Pseudomonas
(3.4%),
Escherichia
(1.7%),
Klebsiella
(1.5%)
as
enriched
biomarkers
DWTS,
which
mainly
carried
beta-lactam,
aminoglycoside
ARGs.
From
further
analysis
ARG-carrying
contigs
(ACCs),
Stenotrophomonas
maltophilia
aeruginosa
most
common
pathogens
among
49
ACC
binning
results
demonstrated
that
33
high-quality
metagenome-assembled
genomes
(MAGs)
discovered
DWTS;
particularly,
MAG
identified
S.
maltophilia-like
(bin.195)
harbored
greatest
number
(n
=
8),
namely,
multidrug
6;
smeD,
semE,
multidrug_transporter,
mexE,
semB,
smeC),
beta-lactam
1;
metallo-beta-lactamase),
[n
aph(3')-IIb].
strong
positive
correlation
between
MGEs
revealed
high
dissemination
risk
Based
on
pure-culture
method,
93
isolates
belong
30
genera
recovered
from
Specifically,
multidrug-resistant
opportunistic
such
P.
aeruginosa,
Bacillus
cereus,
These
insights
into
DWTS's
resistome
indicated
need
for
more
comprehensive
monitoring
management
Furthermore,
effective
disinfection
methods
be
developed
remove
ARGs
DWTSs,
these
findings
could
assist
governing
bodies
surveillance
DWTSs.
