Journal of the Science of Food and Agriculture,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 20, 2024
Osteoporosis
is
a
systemic
skeletal
disorder
characterized
by
decreased
bone
mass
and
impaired
microarchitecture
because
of
an
imbalance
between
resorption
formation.
Existing
pharmacological
treatments
often
have
significant
side
effects
mainly
focus
on
inhibiting
resorption.
Other
than
osteoclast-mediated
resorption,
the
present
study
also
investigates
potential
role
sheepskin
collagen
peptide
(SSCP)
in
formation
promoting
osteoblast
proliferation,
differentiation
mineralization.
Journal of Agricultural and Food Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 10, 2025
Chronic
fluoride
(F)
exposure
is
linked
to
gonadotoxicity
in
females,
yet
the
underlying
molecular
mechanisms
remain
unclear.
This
study
investigated
fluoride-induced
reprotoxicity
using
advanced
genomic
profiling.
RNA-seq
analysis
identified
significant
activation
of
autophagy,
apoptosis,
and
IL-17
signaling
pathways
fluoride-exposed
female
mice.
To
explore
these
mechanisms,
F0
pregnant
mice
were
exposed
deionized
water
(control)
or
100
mg/L
sodium
(NaF)
during
gestation
throughout
F1
generation
(n
=
16
females/group),
covering
puberty
weaning
maturity.
NaF
caused
reductions
body
weight,
organ
coefficients,
pathological
indices,
with
increased
ovarian
autophagic
vacuoles,
mitochondrial
injuries,
elevated
serum/ovary
LPS
levels
females.
qRT-PCR,
fluorescent
staining,
biochemical
assays,
Western
blotting
confirmed
signaling,
autophagy.
Moreover,
16S
rRNA
sequencing
revealed
gut
microbiota
dysbiosis
NaF-exposed
potentially
exacerbating
ovary
injury
via
serum
elevation.
The
could
justify
deteriorated
its
connection
F-induced
injury.
These
findings
provide
mechanistic
insights
into
reprotoxicity,
emphasizing
interplay
apoptosis
disrupting
cellular
homeostasis
suggesting
potential
therapeutic
targets.
Journal of Agricultural and Food Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 16, 2025
Fluoride,
a
common
agricultural
additive
used
to
enhance
plant
resilience
and
pest
control,
poses
toxicity
risks
when
exposure
surpasses
safe
thresholds,
affecting
ecosystems
human
health.
While
its
reproductive
is
recognized,
the
sex-specific
cross-generational
effects
remain
underexplored.
To
address
this
gap,
we
employed
an
integrative
approach
combining
transcriptomics
(next-generation
sequencing
(NGS)),
bioinformatic
network
analysis,
gut
microbiota
sequencing,
in
vivo
functional
assays.
ICR
mice
(F0
generation),
both
male
female,
were
exposed
fluoride
(100
mg/L
drinking
water)
for
35
days,
continuing
through
gestation
offspring
weaning.
Our
transcriptomic
analysis
revealed
significant
upregulation
of
autophagy
(via
PI3K-AKT-mTOR
pathway)
oxidative
stress-induced
mitochondrial
dysfunction
gonadal
tissue,
with
more
pronounced
observed
males.
Further
integrated
analyses
metabolomic
data,
supported
by
experiments,
highlighted
stress,
dysfunction,
pathway
activation
stronger
The
principal
component
confirmed
transcriptome
alterations,
males
showing
substantial
disruption.
Additionally,
16S
rRNA
identified
dysbiosis,
particularly
males,
increased
Firmicutes/Bacteroidetes
ratio
higher
abundances
Oscillospirales
Anaerovoracaceae.
Moreover,
our
study
correlations
between
specific
(e.g.,
Firmicutes,
Proteobacteria)
autophagy,
pathways,
notable
sex-dependent
differences.
These
findings
suggest
that
may
play
critical
role
modulating
fluoride-induced
toxicity,
their
on
stress
cellular
homeostasis.
breakdown
barrier
elevated
serum/gonadal
lipopolysaccharide
(LPS)
levels
fluoride-treated
further
established
link
dysbiosis
toxicity.
underscore
importance
considering
sex
differences
xenobiotic-induced
developmental
Cancer Management and Research,
Год журнала:
2025,
Номер
Volume 17, С. 219 - 237
Опубликована: Фев. 1, 2025
Abstract:
Prostate
cancer
is
prevalent
among
men
aged
65
and
older.
Bone
metastasis
occurs
in
up
to
90%
of
advanced
prostate
patients,
metastatic
generally
considered
a
non-curative
condition
which
can
impact
quality
life.
The
tumor
microenvironment,
comprising
diverse
cellular
non-cellular
elements,
interacts
with
cells
affect
growth
bone
metastasis.
Within
the
different
cell
types,
including
osteoblasts,
osteoclasts,
adipocytes,
endothelial
cells,
hematopoietic
stem
immune
engage
cells.
Some
alter
behavior,
while
others
are
impacted
or
overpowered
by
leading
phases
movement,
dormancy,
latency,
resistance
treatment,
advancement
visible
This
review
summarizes
recent
research
on
microenvironment
metastasis,
exploring
underlying
mechanisms
potential
value
targeting
these
environments
for
treatment.
Keywords:
cancer,
epithelial-mesenchymal
transition,
dormancy
Poultry Science,
Год журнала:
2025,
Номер
104(3), С. 104891 - 104891
Опубликована: Фев. 6, 2025
This
study
aimed
to
investigate
the
effects
of
dietary
fluoride
(F)
and
curcumin
(Cur)
supplementation
on
tibial
biomechanical
performance,
histopathology,
behavior
pullets.
Four
F
levels
(0,
400,
800,
1200
mg/kg)
supplemented
with
CUR
200
were
used
create
8
experimental
groups
in
Hyline
Brown
Behavioral
results
showed
that
supplements
mg/kg
reduced
percentages
feeding,
walking,
standing,
preening
behaviors
while
increasing
percentage
lying
(P
<
0.05).
is
associated
F-induced
elastic
modulus,
maximum
stress,
stiffness
coefficient
toughness
was
increased
cortical
bone
thickened,
trabecular
widened,
excessive
accumulation
collagen
fibers
0.05)
tibia
explained
properties
reduction
Additionally,
loss
antioxidant
capacity
mediated
by
F-accelerated
pathological
damage
Supplementation
alleviated
abnormal
behavior,
expansion
bone,
fibers,
In
conclusion,
level
body,
caused
histopathological
damage,
destroyed
properties,
attenuates
oxidative
rectifies
behavioral
traits.
Ecotoxicology and Environmental Safety,
Год журнала:
2024,
Номер
276, С. 116284 - 116284
Опубликована: Апрель 5, 2024
Fluorosis
due
to
high
fluoride
levels
in
drinking
water
profoundly
affects
the
development
of
human
skeletal
and
dental
structures.
Sodium
butyrate
(NaB)
has
been
found
regulate
overall
bone
mass
prevent
pathological
loss.
However,
mechanism
NaB
action
on
fluorosis
remains
unclear.
In
this
study,
a
rat
model
induced
by
100
mg/L
sodium
was
used
investigate
impact
homeostasis
serum
metabolomics.
It
that
significantly
reduced
resorption
markers
CTX-Ⅰ
TRACP-5B
rats.
Moreover,
increased
calcium
magnesium
bone,
while
decreasing
phosphorus
levels.
addition,
improved
various
microstructure
parameters,
including
mineral
density
(BMD),
trabecular
thickness
(Tb.
Th),
separation
SP),
structural
index
(SMI)
femur.
Notably,
intervention
also
enhanced
antioxidant
capacity
plasma
Furthermore,
comprehensive
analysis
metabolomics
LC-MS
revealed
significant
reversal
trend
seven
biomarkers
after
NaB.
Finally,
pathway
enrichment
based
differential
metabolites
indicated
exerted
protective
effects
modulating
arginine
proline
metabolic
pathways.
These
findings
suggest
beneficial
effect
can
ameliorating
disorders.
