High-Throughput Qpcr Profiling of Antimicrobial Resistance and Bacterial Loads in Wastewater and Receiving Environments: A Risk Assessment
Опубликована: Янв. 1, 2025
Wastewater
treatment
plants
(WWTPs)
are
hot
spots
for
the
acquisition
and
spread
of
antimicrobial
resistance
(AMR).
This
regional-based
study
quantified
antibiotic
genes
(ARGs),
mobile
genetic
elements
(MGEs),
bacteria
in
hospital
community-derived
wastewater
receiving
environments,
using
high-throughput
qPCR
(HT-qPCR).
is
first
to
apply
Resistomap's
Antibiotic
Resistance
Gene
Index
(ARGI)
find
overall
ARG
load
AMR
risk
assessment.
ARGI
WWTPs
ranged
from
2.0
2.3,
indicating
higher
relative
levels
than
mean
European
2.0,
but
lower
global
2.4.
The
highest
diversity
abundance
ARGs
were
observed
untreated
community
wastewater.
reduction
total
during
(0.2-
2
logs)
(0.3-1.5
varied
spatio-temporally
across
WWTPs.
Despite
a
decrease
bacterial
treated
effluents,
substantial
loads
still
released
into
environments.
Notably,
coastal
sediments
comparable
those
wastewater,
most
shared
between
highlighting
impact
discharge
on
these
ecosystems.
Sewage
outfall
exposure
increased
shellfish,
emphasising
risks
shellfish
hygiene.
provides
evidence
inform
policymaking,
advanced
methods
combined
storm
overflow
(CSO)
management
mitigate
release,
protecting
water
users
food
chain.
Язык: Английский
High-Throughput qPCR Profiling of Antimicrobial Resistance Genes and Bacterial Loads in Wastewater and Receiving Environments
Environmental Pollution,
Год журнала:
2025,
Номер
unknown, С. 126096 - 126096
Опубликована: Март 1, 2025
Wastewater
treatment
plants
(WWTPs)
are
hot
spots
for
the
acquisition
and
spread
of
antimicrobial
resistance
(AMR).
This
regional-based
study
quantified
antibiotic
genes
(ARGs),
mobile
genetic
elements
(MGEs),
bacteria
in
hospital
community-derived
wastewater
receiving
environments,
using
high-throughput
qPCR
(HT-qPCR).
is
first
to
apply
Resistomap's
Antibiotic
Resistance
Gene
Index
(ARGI)
as
a
standardised
metric
find
overall
AMR
level
across
different
WWTPs.
ARGI
WWTPs
ranged
from
2.0
2.3,
indicating
higher
relative
ARG
levels
than
mean
European
2.0,
but
lower
global
2.4.
The
highest
diversity
abundance
ARGs
were
observed
untreated
community
wastewater.
reduction
total
during
(0.2-
2
logs)
(0.3-1.5
varied
spatio-temporally
Despite
decrease
bacterial
treated
effluents,
substantial
loads
still
released
into
environments.
Notably,
coastal
sediments
comparable
those
wastewater,
most
shared
between
highlighting
impact
discharge
on
these
ecosystems.
Sewage
outfall
exposure
increased
shellfish,
emphasising
risks
shellfish
hygiene.
provides
evidence
inform
policymaking,
advanced
methods
combined
sewer
overflow
(CSO)
management
mitigate
release,
protecting
water
users
food
chain.
Язык: Английский
Flushed and Forgotten: Antimicrobial Resistance from Wastewater Perspective
IntechOpen eBooks,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 24, 2025
Antimicrobial
resistance
(AMR)
is
a
major
global
health
threat,
reducing
the
effectiveness
of
antibacterial
treatments.
Understanding
its
spread
across
different
environments
crucial
for
improving
surveillance
and
guiding
policies.
Wastewater
an
often
overlooked
but
significant
contributor
to
AMR
crisis.
It
collects
bacteria
from
human,
industrial,
agricultural
sources,
making
it
both
potential
mitigator
through
treatment
pathway
antimicrobial-resistant
(ARB)
genes
(ARGs)
if
not
properly
managed.
The
improper
handling
wastewater
leads
environmental
dissemination
AMR,
exacerbating
spread.
In
many
low-
middle-income
countries
(LMICs),
facilities
are
either
non-existent
or
incapable
effectively
removing
ARB,
ARGs,
antibiotic
residues.
role
in
development
has
been
significantly
underestimated
scientific
research
policy
discussions
because
primarily
concentrated
on
clinical
settings
practices.
A
challenge
addressing
systems
lack
monitoring
frameworks,
which
limits
coordinated
efforts.
Additionally,
remains
underutilized
tool
tracking
trends,
missing
critical
opportunity
early
intervention.
Raising
awareness
about
essential
safeguarding
health.
Strengthening
practices,
implementing
standardized
systems,
integrating
wastewater-based
into
public
strategies
can
help
mitigate
Язык: Английский
Assessment of Antimicrobial Resistance Genes and Pathobiome Diversity in Domestic Wastewater of a Tropical Country
Water,
Год журнала:
2025,
Номер
17(11), С. 1574 - 1574
Опубликована: Май 23, 2025
Wastewater
treatment
plants
(WWTPs)
are
recognised
as
key
hotspots
for
the
dissemination
of
antimicrobial-resistant
bacteria
and
antimicrobial
resistance
genes
(ARGs).
However,
studies
addressing
role
domestic
WWTPs
in
spread
traits
tropical
regions
remain
limited.
This
study
evaluated
a
WWTP
during
dry
rainy
seasons
to
examine
its
reservoir
hub
ARGs
potential
bacterial
pathogens.
The
demonstrated
stable
physicochemical
removal
efficiencies,
although
performance
slightly
decreased
season.
Notably,
relative
abundance
measured
by
qPCR
was
higher
effluent
than
influent
WWTP.
Metagenomic
analysis
activated
sludge
revealed
that
chromosomally
encoded
conferred
macrolides,
aminoglycosides,
rifamycin,
sulphonamides,
tetracyclines.
In
contrast,
plasmid-associated
were
primarily
linked
against
quaternary
ammonium
compounds
(QACs),
indicating
presence
conjugative
plasmid
facilitating
mobility
persistence
QAC
within
microbial
community.
Furthermore,
pathobiome
identified
high
pathogens,
including
genera
Gordonia,
Acidovorax,
Pseudomonas,
Mycobacterium
members.
These
findings
highlight
reservoirs
amplifiers
environments.
Язык: Английский