Microbiome and Resistome in Poultry Litter-Fertilized and Unfertilized Agricultural Soils

Antibiotics, Год журнала: 2025, Номер 14(4), С. 355 - 355

Опубликована: Март 31, 2025

Background: Poultry litter is the main waste of poultry farming and widely used as an agricultural fertilizer. However, owing to use antimicrobials in animal production, it can accumulate antimicrobial residues, antimicrobial-resistant bacteria (ARB), resistance genes (ARGs). This study aimed evaluate impact on microbiome resistome soils. Methods: Soil samples from fertilized unfertilized plots were collected two horticultural farms that intensively litter. Microbiome composition was assessed using 16S rRNA sequencing. A culture-dependent method isolate resistant strains CHROMagar plates supplemented with sulfamethoxazole or ciprofloxacin. ARGs integrase-encoding identified by PCR. Results: analysis revealed significant differences structure between litter-fertilized Fertilized soils exhibited greater alpha diversity richness. Bacillota, commonly found avian gastrointestinal tract, more abundant total 62 isolated, 23 clinically relevant harbored ARGs, including fluoroquinolone (qnrA qnrB) β-lactam (blaGES, blaTEM, blaSHV) genes. Class 1 2 integron-associated (intI1 intI2) also detected. Notably, rare blaGES gene detected Bacillus sp. soil. Similarly, qnrA co-occurred blaSHV a Bosea strain Conclusions: These findings highlight potential for ARB dissemination environments, where once introduced into soils, may spread weathering other environmental factors, complicating negative control selection situ studies.

Язык: Английский

Impacts of anthropogenic disturbances on antibiotic resistomes in biological soil crusts on the Qinghai-Tibetan Plateau

Environmental Pollution, Год журнала: 2024, Номер unknown, С. 125582 - 125582

Опубликована: Дек. 1, 2024

Язык: Английский