Enzyme-based and enzyme-free metal-based glucose biosensors: Classification and recent advances

Microchemical Journal, Год журнала: 2023, Номер 193, С. 109038 - 109038

Опубликована: Июнь 30, 2023

Язык: Английский

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Self-assembled monolayer-assisted label-free electrochemical genosensor for specific point-of-care determination of Haemophilus influenzae

Microchimica Acta, Год журнала: 2023, Номер 190(4)

Опубликована: Март 4, 2023

Язык: Английский

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An Ultrasensitive miRNA-Based Genosensor for Detection of MicroRNA 21 in Gastric Cancer Cells Based on Functional Signal Amplifier and Synthesized Perovskite-Graphene Oxide and AuNPs

Biosensors, Год журнала: 2023, Номер 13(2), С. 172 - 172

Опубликована: Янв. 22, 2023

In the present research work, state-of-art label-free electrochemical genosensing platform was developed based on hybridization process in presence of [Fe(CN)6]3−/4− as an efficient redox probe for sensitive recognition miRNA-21 human gastric cell lines samples. To attain this aim, perovskite nanosheets were initially synthesized. Afterward, obtained compound combined with graphene oxide resulting effective modifier, which dropped surface Au electrode. Then, AuNPs (Gold Nano Particles) have been electrochemically-immobilized perovskite-graphene oxide/Au-modified electrode through chronoamperometry (CA) technique. Finally, a self-assembling monolayer reaction ss-capture RNA ensued by thiol group at end modified surface. has cast to apply process. find out effectiveness synthesized modifier agent, behavior analyzed DPV (differential pulse voltammetry) and CV (cyclic techniques. The prepared biomarker-detection bioassay offers high sensitivity specificity, good performance, appropriate precision accuracy highly-sensitive determination miRNA-21. Different characterization methods used, such XRD, Raman, EDS, FE-SEM, morphological investigation particle size. Based optimal conditions, limit detection quantification acquired 2.94 fM 8.75 fM, respectively. Furthermore, it possible achieve wide linear range is between 10−14 10−7 Moreover, selectivity proposed biosensing assay investigated its potential one, two, three-base mismatched sequences. investigate repeatability reproducibility related bio-assay. evaluate process, important that planned biomarker bio-assay could be directly re-used re-generated.

Язык: Английский

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Synthesis and characterization of Bi2S3-embedded carbon nanofibers as a novel electrochemical biosensor for the detection of mycotoxin zearalenone in food crops

FlatChem, Год журнала: 2024, Номер 45, С. 100652 - 100652

Опубликована: Апрель 2, 2024

Язык: Английский

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Electrochemical aptasensor based on CRISPR/Cas12a-mediated and DNAzyme-assisted cascade dual-enzyme transformation strategy for zearalenone detection

Chemical Engineering Journal, Год журнала: 2024, Номер 493, С. 152431 - 152431

Опубликована: Май 24, 2024

Язык: Английский

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