SARS-CoV-2 in Environmental Samples of Quarantined Households

Viruses, Год журнала: 2022, Номер 14(5), С. 1075 - 1075

Опубликована: Май 17, 2022

The role of environmental transmission SARS-CoV-2 remains unclear. Thus, the aim this study was to investigate whether viral contamination air, wastewater, and surfaces in quarantined households result a higher risk for exposed persons. For study, source population 21 under quarantine conditions with at least one person who tested positive RNA were randomly selected from community North Rhine-Westphalia March 2020. All individuals living these participated provided throat swabs analysis. Air wastewater samples surface obtained each household analysed using qRT-PCR. Positive further cultured analyse infectivity. Out all 43 adults, 26 (60.47%) 15 air qRT-PCR-negative. In total, 10 out 66 (15.15%) 4 119 (3.36%). No statistically significant correlation between qRT-PCR-positive extent spread infection members observed. infectious virus could be propagated cell culture conditions. Taken together, our demonstrates low likelihood via surfaces. However, definitively assess importance hygienic behavioural measures reduction transmission, larger studies should designed determine proportionate contribution smear vs. droplet transmission.

Язык: Английский

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Wastewater surveillance for viral pathogens: A tool for public health

Heliyon, Год журнала: 2024, Номер 10(13), С. e33873 - e33873

Опубликована: Июнь 29, 2024

A focus on water quality has intensified globally, considering its critical role in sustaining life and ecosystems. Wastewater, reflecting societal development, profoundly impacts public health. Wastewater-based epidemiology (WBE) emerged as a surveillance tool for detecting outbreaks early, monitoring infectious disease trends, providing real-time insights, particularly vulnerable communities. WBE aids tracking pathogens, including viruses, sewage, offering comprehensive understanding of community health lifestyle habits. With the rise global COVID-19 cases, gained prominence, aiding SARS-CoV-2 levels worldwide. Despite advancements treatment, poorly treated wastewater discharge remains threat, amplifying spread water-, sanitation-, hygiene (WaSH)-related diseases. WBE, serving complementary surveillance, is pivotal community-level viral infections. However, there untapped potential to expand surveillance. This review emphasizes importance link between health, highlighting need further integration into management.

Язык: Английский

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An opinion on Wastewater-Based Epidemiological Monitoring (WBEM) with Clinical Diagnostic Test (CDT) for detecting high-prevalence areas of community COVID-19 infections

Current Opinion in Environmental Science & Health, Год журнала: 2022, Номер 31, С. 100396 - 100396

Опубликована: Окт. 6, 2022

Wastewater-Based Epidemiological Monitoring (WBEM) is an efficient surveillance tool during the COVID-19 pandemic as it meets all requirements of a complete monitoring system including early warning, tracking current trend, prevalence disease, detection genetic diversity well asthe up-surging SARS-CoV-2 new variants with mutations from wastewater samples. Subsequently, Clinical Diagnostic Test widely acknowledged global gold standard method for disease monitoring, despite several drawbacks such high diagnosis cost, reporting bias, and difficulty asymptomatic patients (silent spreaders infection who manifest nosymptoms disease). In this reviewand opinion-based study, we first propose combined approach) detecting in communities using clinical sample testing, which may be feasible effective emerging public health long-term nationwide system. The viral concentrations samples can used indicatorsto monitor ongoing trends, predict carriers, detect hotspot areas, while sampleshelp mostlysymptomaticindividuals isolating positive cases validate WBEM protocol mass vaccination booster doses COVID-19.

Язык: Английский

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Presence and persistence of SARS-CoV-2 in aquatic environments: A mini-review

Current Opinion in Environmental Science & Health, Год журнала: 2022, Номер 29, С. 100385 - 100385

Опубликована: Июль 31, 2022

The introduction of SARS-CoV-2 into water bodies via sewage raises public health concerns. For the assessment risks, it is necessary to know presence and persistence infectious in wastewater. present mini-review documents occurrence decay rates viable RNA different matrices including wastewater, river water, groundwater, tap seawater. Persistence mainly temperature dependent. A rapid inactivation found sea wastewater compared water. was be considerably more stable than SARS-CoV-2, indicating that environmental detection alone does not prove risk infection. assays need consider physicochemical biological composition as well effect detergents, enzymes, filtering particulate matter.

Язык: Английский

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Wastewater early warning system for SARS-CoV-2 outbreaks and variants in a Coruña, Spain

Environmental Science and Pollution Research, Год журнала: 2023, Номер 30(32), С. 79315 - 79334

Опубликована: Июнь 7, 2023

Abstract Wastewater-based epidemiology has been widely used as a cost-effective method for tracking the COVID-19 pandemic at community level. Here we describe COVIDBENS, wastewater surveillance program running from June 2020 to March 2022 in treatment plant of Bens A Coruña (Spain). The main goal this work was provide an effective early warning tool based help decision-making both social and public health levels. RT-qPCR procedures Illumina sequencing were weekly monitor viral load detect SARS-CoV-2 mutations wastewater, respectively. In addition, own statistical models applied estimate real number infected people frequency each emerging variant circulating community, which considerable improved strategy. Our analysis detected 6 waves with concentrations between 10 3 RNA copies/L. system able anticipate outbreaks during 8–36 days advance respect clinical reports and, emergence new variants such Alpha (B.1.1.7), Delta (B.1.617.2), Omicron (B.1.1.529 BA.2) 42, 30, 27 days, respectively, before did. Data generated here helped local authorities managers give faster more efficient response situation, also allowed important industrial companies adapt their production situation. wastewater-based developed our metropolitan area (Spain) served powerful combining monitoring over time.

Язык: Английский

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View of an Evolving Pandemic: Changes in the Relationship Between Clinical Cases and Levels of SARS-CoV-2 RNA in Colorado Wastewater

ACS ES&T Water, Год журнала: 2024, Номер 4(5), С. 2018 - 2030

Опубликована: Апрель 16, 2024

The utility and interpretation of SARS-CoV-2 wastewater monitoring data as a predictor community health can be confounded by variables such system complexity, viral variants, human behavior, including vaccination status use at-home tests. Here, we explored the relationship between COVID caseload concentration in for 23 locations over 22-month period pandemic evolved. Spearman's rank analysis showed strong correlations (ρ > 0.7) most facilities, independent normalization. While remained throughout pandemic, application change point (CPA) identified shifts reported clinical cases signal time. These did not generally coincide with known milestones, suggesting involvement multiple interacting or unknown variables. Models accounting these phase significantly improved predictions caseloads. Additionally, existence points highlights increased reliability when changes ratio to concentrations are due immunity behaviors. In future pandemics, public professionals will ideally aware that case-to-copy unpredictably pandemics evolve, CPA support decision-making.

Язык: Английский

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Detection, Quantification, and Simplified Wastewater Surveillance Model of SARS-CoV-2 RNA in the Tijuana River

ACS ES&T Water, Год журнала: 2022, Номер 2(11), С. 2134 - 2143

Опубликована: Апрель 26, 2022

The COVID-19 pandemic and the detection of SARS-CoV-2 RNA in sewage has expanded global interest wastewater surveillance. However, many underserved communities throughout world lack improved sanitation use informal combined sanitary storm sewer systems. Sewage is transported via open channels, ditches, rivers, where it mixes with surface water and/or stormwater. There a need to develop better methods for surveillance pathogens such as this context. We developed simplified system monitored flow rates concentrations Tijuana River at two locations downstream United States-Mexico border California, States. was detected upstream location on six out eight occasions, which were high those reported untreated from California virus not any samples collected (estuarine) sampling location, despite consistent PMMoV RNA. Synchrony observed between number cases measured CDC N1 assay when latter normalized by river.

Язык: Английский

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Environmental surveillance and wastewater-based epidemiology for infectious diseases in Low and Middle Income Countries: A scoping review of mathematical modelling and analytical studies

Опубликована: Фев. 7, 2025

Язык: Английский

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The potential of long‐term wastewater‐based surveillance to predict COVID‐19 waves peak in Mexico

Water Environment Research, Год журнала: 2025, Номер 97(5)

Опубликована: Май 1, 2025

Abstract Wastewater‐based surveillance (WBS) is valuable method for monitoring the dispersion of pathogens at a low cost. However, their impact on public health decision‐making limited because there lack long‐term analyses, especially in low‐ and middle‐income countries. This study aimed to assess effectiveness using WBS predict occurrence COVID‐19 waves estimate prevalence infection, emphasizing SARS‐CoV‐2 variants. During 17 months influent two wastewater treatment plants Queretaro City, Mexico, wave prediction time was influenced by variant dispersion. Waves dominated Delta Omicron variants circulation showed lead days values from 5 14 1 4 days, respectively. According Monte Carlo model, disease aligned with clinically reported cases onsets, but variant's transmissibility explained overestimation during peaks. work provides new insights into potential limitations as an epidemiological tool detecting predicting occurrence. Practitioner Points Long‐term allowed early case waves. The capability related presence infectivity. estimated higher all estimation has regarding variations data input.

Язык: Английский

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Relationship between SARS-CoV-2 in wastewater and clinical data from five wastewater sheds

Journal of Hazardous Materials Advances, Год журнала: 2022, Номер 8, С. 100159 - 100159

Опубликована: Сен. 7, 2022

The Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has resulted in a global pandemic starting 2019 with nearly 500 million confirmed cases as of April 2022. Infection SARS-CoV-2 is accompanied by shedding virus stool, and its presence wastewater samples been documented globally. Therefore, monitoring offers promising approach to assess the situation covering pre-symptomatic asymptomatic areas limited clinical testing. In this study, RNA from five resource recovery facilities (WRRFs), located two adjacent counties, was investigated compared number COVID-19 during 2020-2021 outbreak United States. Statistical correlation analyses viral abundance daily vs weekly performed. While weak on basis observed, improved when case data were applied. fecal indicator Pepper Mild Mottle Virus (PMMoV) furthermore used effects normalization impact dilution due infiltration sheds. Normalization did not improve correlations data. However, PMMoV provided important information about industrial discharge This study showed utility WBE assist public health responses COVID-19, emphasizing that routine large WRRFs could provide sufficient for large-scale dynamics.

Язык: Английский

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