Wastewater-based surveillance as a tool for public health action: SARS-CoV-2 and beyond

Clinical Microbiology Reviews, Год журнала: 2023, Номер 37(1)

Опубликована: Дек. 14, 2023

SUMMARY Wastewater-based surveillance (WBS) has undergone dramatic advancement in the context of coronavirus disease 2019 (COVID-19) pandemic. The power and potential this platform technology were rapidly realized when it became evident that not only did WBS-measured SARS-CoV-2 RNA correlate strongly with COVID-19 clinical within monitored populations but also, fact, functioned as a leading indicator. Teams from across globe innovated novel approaches by which wastewater could be collected diverse sewersheds ranging treatment plants (enabling community-level surveillance) to more granular locations including individual neighborhoods high-risk buildings such long-term care facilities (LTCF). Efficient processes enabled extraction concentration highly dilute matrix. Molecular genomic tools identify, quantify, characterize its various variants adapted programs applied these mixed environmental systems. Novel data-sharing allowed information mobilized made immediately available public health government decision-makers even public, enabling evidence-informed decision-making based on local dynamics. WBS since been recognized tool transformative potential, providing near-real-time cost-effective, objective, comprehensive, inclusive data changing prevalence measured analytes space time populations. However, consequence rapid innovation hundreds teams simultaneously, tremendous heterogeneity currently exists literature. This manuscript provides state-of-the-art review established details current work underway expanding scope other infectious targets.

Язык: Английский

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Small-scale wastewater-based epidemiology (WBE) for infectious diseases and antibiotic resistance: A scoping review

International Journal of Hygiene and Environmental Health, Год журнала: 2024, Номер 259, С. 114379 - 114379

Опубликована: Апрель 15, 2024

Wastewater analysis can serve as a source of public health information. In recent years, wastewater-based epidemiology (WBE) has emerged and proven useful for the detection infectious diseases. However, insights from wastewater treatment plant do not allow small-scale differentiation within sewer system that is needed to analyze target population under study in more detail. Small-scale WBE offers several advantages, but there been no systematic overview its application. The aim this scoping review provide comprehensive current state knowledge on diseases, including methodological considerations A database search was conducted, considering only peer-reviewed articles. Data analyses included quantitative summary qualitative narrative synthesis. Of 2130 articles, we 278, most which were published since 2020. studies analyzed at building level (n = 203), especially healthcare 110) educational facilities 80), neighborhood scale 86). main analytical parameters viruses 178), notably SARS-CoV-2 161), antibiotic resistance (ABR) biomarkers 99), often by polymerase chain reaction (PCR), with DNA sequencing techniques being less common. terms sampling techniques, active dominated. frequent lack detailed information specification selection criteria characterization sites identified concern. conclusion, based large number studies, overarching strategic aspects WBE. An enabling environment requires inter- transdisciplinary sharing across countries. Promoting adoption will benefit common international conceptualization approach, standardized internationally accepted terminology. particular, development good practices different warranted. This includes establishment guidelines local sub-sewersheds, transparent reporting ensure comparability results.

Язык: Английский

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Wastewater surveillance could serve as a pandemic early warning system for COVID‐19 and beyond

Wiley Interdisciplinary Reviews Water, Год журнала: 2023, Номер 10(4)

Опубликована: Май 8, 2023

Abstract Wastewater‐based surveillance can be used as an early warning system to identify COVID‐19 outbreaks because the viral load observed in sewage before it is clinically verified. Wastewater of SARS‐CoV‐2 trace transmission dynamics infection communities when using scale a wastewater diversion and treatment system. Using this detection method help protect human health mitigate socio‐economic losses. It quantify epidemiological data given population real‐time circumvent need for other indicators. There are challenges technique areas with underdeveloped sewerage infrastructure. especially case developing nations where uniform protocols lacking, heterogeneous environmental operational conditions. This article explains importance wastewater‐based SARS‐CoV‐2. lays out most recent methodological approaches detecting municipal outlines main associated epidemiology (WBE). The includes study work across India demonstrate how nation manages research locational challenges. socio‐economic, ethical, policy dimensions WBE also discussed. categorized under: Engineering Water > Water, Health, Sanitation Sustainable Methods

Язык: Английский

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Significance of wastewater surveillance in detecting the prevalence of SARS-CoV-2 variants and other respiratory viruses in the community – A multi-site evaluation

One Health, Год журнала: 2023, Номер 16, С. 100536 - 100536

Опубликована: Апрель 6, 2023

Detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral genome in wastewater has proven to be useful for tracking the trends virus prevalence within community. The surveillance also provides precise and early detection any new circulating variants, which aids response outbreaks. Site-specific monitoring SARS-CoV-2 variants valuable information on or emerging We sequenced genomic RNA viruses present samples analyzed as well other a period one year account seasonal variations. were collected from Reno-Sparks metropolitan area weekly basis between November 2021 2022. Samples detect levels copies identification. This study confirmed that can used community supports wastewater-based epidemiology (WBE) complement clinical testing healthcare effort. Our showed persistence throughout compared presence viruses, implicating SARS-CoV-2's broad genetic diversity strength persist infect susceptible hosts. Through secondary analysis, we further identified antimicrobial resistance (AMR) genes same found WBE feasible tool AMR monitoring.

Язык: Английский

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Quantification of SARS-CoV-2 in wastewater samples from hospitals treating COVID-19 patients during the first wave of the pandemic in Brazil

The Science of The Total Environment, Год журнала: 2022, Номер 860, С. 160498 - 160498

Опубликована: Ноя. 25, 2022

Язык: Английский

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Long-term passive wastewater surveillance of SARS-CoV-2 for seven university dormitories in comparison to municipal surveillance

The Science of The Total Environment, Год журнала: 2022, Номер 852, С. 158421 - 158421

Опубликована: Сен. 1, 2022

Язык: Английский

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Wastewater monitoring for detection of public health markers during the COVID-19 pandemic: Near-source monitoring of schools in England over an academic year

PLoS ONE, Год журнала: 2023, Номер 18(5), С. e0286259 - e0286259

Опубликована: Май 30, 2023

Schools are high-risk settings for infectious disease transmission. Wastewater monitoring diseases has been used to identify and mitigate outbreaks in many near-source during the COVID-19 pandemic, including universities hospitals but less is known about technology when applied school health protection. This study aimed implement a wastewater surveillance system detect SARS-CoV-2 other public markers from schools England.

Язык: Английский

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Identifying spatiotemporal trends of SARS-CoV-2 RNA in wastewater: from the perspective of upstream and downstream wastewater-based epidemiology (WBE)

Environmental Science and Pollution Research, Год журнала: 2024, Номер 31(8), С. 11576 - 11590

Опубликована: Янв. 14, 2024

Язык: Английский

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Localised wastewater SARS-CoV-2 levels linked to COVID-19 cases: A long-term multisite study in England

The Science of The Total Environment, Год журнала: 2025, Номер 962, С. 178455 - 178455

Опубликована: Янв. 1, 2025

Wastewater-based surveillance (WBS) can monitor for the presence of human health pathogens in population. During COVID-19, WBS was widely used to determine wastewater SARS-CoV-2 RNA concentration (concentrations) providing information on community COVID-19 cases (cases). However, studies examining relationship between concentrations and tend be localised or focussed small-scale institutional settings. Few have examined this multiple settings, over long periods, with large sample numbers, nor attempted quantify detail how catchment characteristics affected these. This 18-month study (07/20-12/21) explored correlation quantitative using censored regression. Our analysis >94,000 samples collected from 452 diverse sampling sites (259 Sewage Treatment Works (STW) 193 Sewer Network Sites (SNS)) covering ~65 % English Wastewater were linked ~6 million diagnostically confirmed cases. High coefficients found (STW: median r = 0.66, IQR: 0.57-0.74; SNS: 0.65, 0.54-0.74). The (regression coefficient) variable catchments. Catchment (e.g. size population grab vs automated sampling) had significant but small effects regression coefficients. last six months reduced became highly coincided a shift towards younger cases, vaccinated rapid emergence variant Omicron. programme rapidly introduced at scale during COVID-19. Laboratory methods evolved catchments characteristics. Despite diversity, findings indicate that provides an effective proxy establishing dynamics across wide variety communities. While there is potential predicting concentration, may more smaller scales.

Язык: Английский

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Environmental Surveillance of Vector-Borne Diseases in a Non-Sewered System: A Case Study in Mozambique

Environmental Science & Technology, Год журнала: 2025, Номер unknown

Опубликована: Фев. 14, 2025

Arboviral diseases pose major economic and social threats in less economically developed countries (LEDCs), where monitoring is challenging, especially rapidly growing cities with informal settlements. In this study, we aimed to explore environmental surveillance (ES) a non-sewered setting as complement syndromic Maputo, Mozambique. Water samples were collected from nine points along the Infulene River (n = 66) Mozambique February September 2023. The presence of arboviruses (Dengue (DENV), Chikungunya (CHIKV), West Nile (WNV), Usutu (USUV) virus) was determined by RT-qPCR. For specific detection CHIKV, two RT-qPCR assays used: Nsp1, targeting non-structural protein 1 gene (nsP1) E1, E1 envelope (E1). DENV detected 82% (54/66) samples, median viral RNA load 2.7 × 10-2 (2.2 105 copies/L (cp/L)), while CHIKV detectable 98% (65/66) 4.8 cp/L) for nsP1 8.0 (4.8 cp/L), USUV 6% (4/66) at 4.1 10-7 (0 cP/L), positive varying between 1.8 10-3 (7.1 102 4.95 (2.1 103 cp/L). WNV not throughout study. prevalence concentration varied across sampling dates. Our study demonstrated potential ES tool assessing circulation Mozambique, sewered system unavailable. Consequently, could be expanded polio include other targets LEDCs.

Язык: Английский

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