Thermal fluctuations independently modulate physiological plasticity and the dynamics of the gut microbiome in a tropical rocky shore oyster DOI Creative Commons
Bovern Suchart Arromrak, Adrian Tsz Chun Wong, Tin Yan Hui

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Фев. 8, 2023

Abstract Extreme high thermal conditions on tropical rocky shores are challenging to the survival of intertidal ectotherms. Yet, many species highly successful in these environments part due their ability regulate intrinsic mechanisms associated with physiological stress and metabolic demand. More recently, there has been a growing awareness that other extrinsic mechanisms, such as animal-associated microbial communities, can also influence tolerance ectotherms under stressful conditions. However, extent which functionally linked overall adaptive response animals temperature change is poorly understood. Here, we examined dynamics potential interactions high-supratidal oyster, Isognomon nucleus . We found oysters modulate internal biochemistry (oxidized PUFA products, including 5-F 2t -IsoP, 10-F 4t -NeuroP, 13-F 16-F 1t -PhytoP) regulation cope during periods extreme temperatures when emersed. while detected variation alpha diversity (ASV richness Shannon index), dominant taxa functions across time, no association was host biochemical profiles. The findings here suggest condition within independently responses microbiome Together, may contribute zone.

Язык: Английский

Evaluating survival after heat challenges and investigating their correlations with field summer survival for the juvenile Pacific oyster (Crassostrea gigas) DOI
Yong Chi,

Chengxun Xu,

Qi Li

и другие.

Aquaculture, Год журнала: 2024, Номер 584, С. 740643 - 740643

Опубликована: Фев. 2, 2024

Язык: Английский

Процитировано

2
Thermal fluctuations independently modulate physiological plasticity and the dynamics of the gut microbiome in a tropical rocky shore oyster DOI
Bovern Suchart Arromrak, Adrian Tsz Chun Wong, Tin Yan Hui

и другие.

Journal of Experimental Marine Biology and Ecology, Год журнала: 2024, Номер 573, С. 152004 - 152004

Опубликована: Фев. 22, 2024

Язык: Английский

Процитировано

2
Characterization of water microbiota and their relationship with resident oysters during an oyster mortality event DOI Creative Commons
Mingkun Liu, Qingyuan Li,

Xu Wenwen

и другие.

Microbiology Spectrum, Год журнала: 2024, Номер 12(10)

Опубликована: Авг. 20, 2024

ABSTRACT Microorganisms are vital for the health of marine invertebrates, and their assembly is driven by both deterministic stochastic factors that regulate residents (innate to host) transients (from ambient water). However, role water microbiota significance processes in aquatic hosts facing mortality threats largely unknown. This study examines shifts during an oyster event using amplicon sequencing compared with those resident oysters disentangle balance involved. Water temperature dissolved oxygen significantly shape microbial community a distinct monthly pattern, Cyanobacteria blooms might exacerbate mortality. The comparative analysis communities revealed ≤ 21% genera were shared between water, implying cannot easily transfer into oysters. Furthermore, these had different functions, more involved promoting host digestion nutrient acquisition bacteria enriched functions own growth survival. These findings illustrate may possess specific selection or barrier mechanisms permit small percentage transients, controlled having minimal effect on mortality, enter, whereas majority governed factors. Consequently, exhibit some plasticity symbiotic microbiota, enabling them maintain homeostasis adapt complex surroundings. be mechanism among invertebrates survival environments. IMPORTANCE Pacific widely cultured play ecological roles. summer hinders sustainable farming. Untangling causative task due intricate “interactome” involving environmental factors, hosts, pathogens. Interactions microorganisms offer ideal avenue investigating truth. We systematically investigated proposed primarily independent Pathogens mainly originate from members limited influence water. Additionally, degraders, such as blooms, overlooked contributing factor evaluated weight greatly broadened our understanding through interaction microbiota.

Язык: Английский

Процитировано

1
The intra-tumoral microbiome as a potential biomarker of response to external beam radiation therapy in cervical cancer DOI Creative Commons

Zhongyan Dou,

Conghui Ai,

Jinping Zhang

и другие.

Journal of Translational Medicine, Год журнала: 2024, Номер 22(1)

Опубликована: Окт. 28, 2024

We aimed to determine the potential predictive value of intra-tumoral microbiome as a marker response external beam radiation therapy (EBRT) in cervical cancer (CC). A prospective longitudinal trial 36 CC patients receiving pelvic radiotherapy was designed investigate microbial characteristic signatures and diversity (alpha beta) multiple sites (tumor, vaginal, gut, urethral, oral) superior (SR) inferior (IR) groups by 16S rRNA sequencing. Utilized least absolute shrinkage selection operator (LASSO) logistic regression method analyze clinicopathological factors that potentially influenced efficacy EBRT. LEfSe analysis highlighted features best distinguished categorized patient samples. Selected parameters were validated with Spearman correlation analysis, receiver operating (ROC) area under curve (AUC) Kaplan-Meier survival analysis. Firstly, our cohort, LASSO revealed no association between EBRT efficacy. Subsequently, we employed sequencing compare differences across their correlations major factors. discovered independent clinicopathologic represented most direct reliable reflection SR IR groups. found lower alpha tumor group identified relevant taxa (Bifidobacteriaceae, Beijerinckiaceae, Orbaceae) associated patients. then conducted ROC finding specific had an AUC 0.831 (95% CI, 0.667–0.995), indicating these biomarkers for predicting showed better prognosis higher relative abundance Bifidobacteriaceae. Our data suggested may play important role sensitivity offer novel

Язык: Английский

Процитировано

1
Antimicrobial Resistance Profile of Planctomycetota Isolated from Oyster Shell Biofilm: Ecological Relevance within the One Health Concept DOI Creative Commons
Bárbara Guedes, Ofélia Godinho, Sandra Quinteira

и другие.

Applied Microbiology, Год журнала: 2023, Номер 4(1), С. 16 - 26

Опубликована: Дек. 20, 2023

Background: Planctomycetota isolation in pure culture is still challenging with most of the reported data coming from molecular-based methods. Here, we intended to isolate filter-feeder Pacific oyster Magallana gigas, extending search a not yet explored natural reservoir and characterize their antimicrobial resistance phenotype. Methods: Oyster samples different supermarkets farm producer were subject selective medium. Inoculation was performed shell biofilm after an enrichment edible content. Results: isolates (n = 65) only obtained four species identified: Rhodopirellula baltica 62), rubra 1), heiligendammensis 1) Gimesia chilikensis 1). This study reports first association members oysters description R. Portugal. Moreover, rubra, originally identified Portugal, isolated French origin. Antibiotic susceptibility testing, conducted strains belonging two never assayed before revealed multidrug phenotypes bacteria showing several classes clinically relevant antibiotics (e.g., β-lactams aminoglycosides). Conclusion: The ecological role impact on holobiont and, ultimately, public health, under One Health concept, discussed.

Язык: Английский

Процитировано

2
Thermal fluctuations independently modulate physiological plasticity and the dynamics of the gut microbiome in a tropical rocky shore oyster DOI Creative Commons
Bovern Suchart Arromrak, Adrian Tsz Chun Wong, Tin Yan Hui

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Фев. 8, 2023

Abstract Extreme high thermal conditions on tropical rocky shores are challenging to the survival of intertidal ectotherms. Yet, many species highly successful in these environments part due their ability regulate intrinsic mechanisms associated with physiological stress and metabolic demand. More recently, there has been a growing awareness that other extrinsic mechanisms, such as animal-associated microbial communities, can also influence tolerance ectotherms under stressful conditions. However, extent which functionally linked overall adaptive response animals temperature change is poorly understood. Here, we examined dynamics potential interactions high-supratidal oyster, Isognomon nucleus . We found oysters modulate internal biochemistry (oxidized PUFA products, including 5-F 2t -IsoP, 10-F 4t -NeuroP, 13-F 16-F 1t -PhytoP) regulation cope during periods extreme temperatures when emersed. while detected variation alpha diversity (ASV richness Shannon index), dominant taxa functions across time, no association was host biochemical profiles. The findings here suggest condition within independently responses microbiome Together, may contribute zone.

Язык: Английский

Процитировано

0