Circular Single-Stranded DNA: Discovery, Biological Effects, and Applications
ACS Synthetic Biology,
Год журнала:
2024,
Номер
13(4), С. 1038 - 1058
Опубликована: Март 19, 2024
The
field
of
nucleic
acid
therapeutics
has
witnessed
a
significant
surge
in
recent
times,
as
evidenced
by
the
increasing
number
approved
genetic
drugs.
However,
current
platform
technologies
containing
plasmids,
lipid
nanoparticle-mRNAs,
and
adeno-associated
virus
vectors
encounter
various
limitations
challenges.
Thus,
we
are
devoted
to
finding
novel
vector
have
directed
our
efforts
toward
investigating
circular
single-stranded
DNA
(CssDNA),
an
ancient
form
acid.
CssDNAs
ubiquitous,
but
generally
ignored.
Accumulating
evidence
suggests
that
possess
exceptional
properties
vectors,
exhibiting
great
potential
for
clinical
applications
disorders,
gene
editing,
immune
cell
therapy.
Here,
comprehensively
review
discovery
biological
effects
well
their
biomedical
research
first
time.
Undoubtedly,
DNA,
CssDNA
holds
immense
promises
insights
research.
Язык: Английский
Evaluation of humoral and cellular immune responses against Vibrio cholerae using oral immunization by multi-epitope-phage-based vaccine
International Immunopharmacology,
Год журнала:
2024,
Номер
134, С. 112160 - 112160
Опубликована: Май 5, 2024
Язык: Английский
Blocking Fusobacterium nucleatum-host cell interactions with responsive supramolecular GalNAc-derived nanoplatform for enhanced chemotherapy in colorectal cancer
Nano Today,
Год журнала:
2024,
Номер
56, С. 102288 - 102288
Опубликована: Май 6, 2024
Язык: Английский
Developing recombinant antibodies by phage display technology to neutralize viral infectious diseases
SLAS DISCOVERY,
Год журнала:
2024,
Номер
29(3), С. 100140 - 100140
Опубликована: Янв. 3, 2024
The
use
of
recombinant
antibodies
developed
through
phage
display
technology
offers
a
promising
approach
for
combating
viral
infectious
diseases.
By
specifically
targeting
antigens
on
surfaces,
these
have
the
potential
to
reduce
severity
infections
or
even
prevent
them
altogether.
With
emergence
new
and
more
virulent
strains
viruses,
it
is
crucial
develop
innovative
methods
counteract
them.
Phage
has
proven
successful
in
generating
capable
specific
antigens,
thereby
providing
powerful
tool
fight
infections.
In
this
mini-review
article,
we
examine
development
using
technology,
discuss
associated
challenges
opportunities
developing
novel
treatments
Furthermore,
provide
an
overview
technology.
As
continue
evolve
improve,
sophisticated
tools
based
peptide
systems
are
constantly
emerging,
offering
exciting
prospects
solving
scientific,
medical,
technological
problems
related
diseases
near
future.
Язык: Английский
Development and application of an interbacterial DNA delivery system based on M13 bacteriophage
Archives of Microbiology,
Год журнала:
2025,
Номер
207(5)
Опубликована: Апрель 5, 2025
Язык: Английский
Harnessing filamentous phages for enhanced stroke recovery
Frontiers in Immunology,
Год журнала:
2024,
Номер
14
Опубликована: Янв. 16, 2024
Stroke
poses
a
critical
global
health
challenge,
leading
to
substantial
morbidity
and
mortality.
Existing
treatments
often
miss
vital
timeframes
encounter
limitations
due
adverse
effects,
prompting
the
pursuit
of
innovative
approaches
restore
compromised
brain
function.
This
review
explores
potential
filamentous
phages
in
enhancing
stroke
recovery.
Initially
antimicrobial-centric,
bacteriophage
therapy
has
evolved
into
regenerative
solution.
We
explore
diverse
role
post-stroke
neurological
restoration,
emphasizing
their
ability
integrate
peptides
phage
coat
proteins,
thereby
facilitating
Experimental
evidence
supports
efficacy
alleviating
complications,
immune
modulation,
tissue
regeneration.
However,
rigorous
clinical
validation
is
essential
address
challenges
like
dosing
administration
routes.
Additionally,
genetic
modification
enhances
as
injectable
biomaterials
for
complex
issues.
emphasizes
strategies
capacity
contribute
enhanced
recovery,
opposed
serving
standalone
treatment,
particularly
addressing
stroke-induced
damage.
Язык: Английский
Nanomicelle carrier developed using biosurfactants: a novel approach for selective gene targeting
International Journal of Polymeric Materials,
Год журнала:
2024,
Номер
unknown, С. 1 - 12
Опубликована: Март 6, 2024
Gene
therapy
has
emerged
as
a
promising
avenue
for
treating
genetic
disorders,
cancer,
and
other
diseases
by
introducing
therapeutic
genes
or
modulating
gene
expression.
However,
the
success
of
critically
depends
on
efficient
safe
delivery
systems.
This
review
explores
nanomicellar
formulations
with
biosurfactants
innovative
targeting.
Traditional
systems
face
issues
in
safety,
immunogenicity,
efficiency.
Biosurfactants
not
only
enhance
biocompatibility
carriers
but
also
contribute
to
their
sustainability.
T
Nanomicelles,
self-assembling
nanoscale
structures
formed
amphiphilic
molecules,
provide
solution.
They
offer
small
size,
high
surface
area,
encapsulation
capabilities
precise
delivery.
The
inclusion
enhances
covers
nanomicelle
formation
principles,
biosurfactant
features,
applications,
along
recent
advances
challenges.
synergy
between
potential
revolutionize
regenerative
medicine,
solutions
improved
human
health.
Язык: Английский
The enhancement of M13 phage titration by optimizing the origin of replication
Research in Pharmaceutical Sciences,
Год журнала:
2024,
Номер
19(3), С. 338 - 346
Опубликована: Май 1, 2024
Background
and
purpose:
M13KO7,
a
modified
M13
phage
variant,
carries
the
p15A
replication
origin
Tn903
kanamycin
resistance
gene.
This
study
aimed
to
optimize
M13KO7's
by
substituting
with
higher-copy
pMB1
(500-700
copy
numbers).
Experimental
approach:
A
6431-nucleotide
fragment
from
M13KO7
plasmid
lacking
gene
was
amplified
using
long
polymerase
chain
reaction
(PCR).
The
M13AMB1
created
adding
adenine
3’
ends
of
this
ligating
it
pMB1-containing
T/A
cloning.
Afterward,
prepare
phage,
pM13AMB1
transformed
into
E.
coli
TG1
bacteria,
then,
PEG-NaCl
precipitation,
propagated.
titer
determined
utilizing
serial
dilution
qPCR
methods,
compared
phage.
Findings/Results:
results
showed
that
in
method,
titers
were
4.8
×
10
14
7
12
pfu/mL,
respectively.
Besides,
calculated
method
for
equal
1.3
9
whereas
4.08
8
pfu/mL
Conclusion
implications:
provides
evidence
replacement
led
significant
increase
titers.
It
highlights
importance
optimization
molecular
biology
applications.
Язык: Английский
Male-specific bacteriophages and their potential on combating the spreading of T4SS-bearing antimicrobial resistance plasmids
Critical Reviews in Microbiology,
Год журнала:
2024,
Номер
unknown, С. 1 - 12
Опубликована: Сен. 10, 2024
Antimicrobial
resistance
(AMR)
has
been
recognized
as
an
important
health
crisis
in
the
twenty
first
century.
Type
IV
secretion
systems
(T4SSs)
play
key
roles
dissemination
of
AMR
plasmids.
Novel
strategies
that
combat
problem
by
targeting
T4SS
sprung
up
recent
years.
Here,
we
focus
on
strategy
male-specific
phages
could
target
and
kill
bacteria
carrying
conjugative
plasmids
encoding
T4SSs.
We
reviewed
advances
phages,
including
anti-conjugation
mechanisms,
clinical
isolation
identification
methods,
classification
characteristics,
Язык: Английский