Start-up of anammox in an up-flow anaerobic sludge bed reactor: bacterial community composition and antibiotic resistance genes
Abstract
Anaerobic
ammonia
oxidation
(anammox)
is
considered
a
high-efficiency
and
low-consumption
biological
nitrogen
removal
process.
In
this
study,
synthetic
wastewater
was
used
to
start
an
up-flow
anaerobic
sludge
bed
reactor
with
starting
temperature
of
20–31℃,
subsequently,
the
activated
samples
were
analyze
changes
in
bacterial
community
antibiotic
resistance
genes
(ARGs)
by
metagenomic
sequencing.
The
results
showed
that
reaction
successfully
started
up
after
132
days
cultivation,
achieving
NH4+-N
NO2−-N
rates
over
99.5%.
Candidatus
Kuenenia,
anammox
bacterium,
increased
from
0.01
50.86%.
denitrifying
bacteria
Delftia ,
Acidovorax ,
Thauera
Alicycliphilus
decreased
18.70,
8.02,
4.94
4.59%
7.01,
1.77,
3.06
3.96%,
respectively.
ammonia-oxidizing
bacterium
Nitrosomonas
2.91
1.87%.
After
relative
abundance
ARGs
90.23
64.29
ppm,
sulfonamide,
macrolide-lincosamide-streptogramin,
tetracycline,
aminoglycoside
multidrug
being
main
types.
Additionally,
ARG
subtypes
sul1 ,
msrE
tetX
decreased,
while
ermF ,
sul2
floR
increased.
These
contribute
knowledge
performance,
composition
reactor,
providing
guidance
for
anammox.
Research Square (Research Square), Год журнала: 2024, Номер unknown
Опубликована: Июнь 13, 2024
Язык: Английский
