An innovative approach for marine macro-organism monitoring: methodology and future perspectives of environmental DNA (eDNA) technology

Marine Biology, Год журнала: 2025, Номер 172(3)

Опубликована: Март 1, 2025

Язык: Английский

Long-distance Southern Ocean environmental DNA (eDNA) transect provides insights into spatial marine biota and invasion pathways for non-native species

The Science of The Total Environment, Год журнала: 2024, Номер 951, С. 175657 - 175657

Опубликована: Авг. 22, 2024

The Southern Ocean surrounding Antarctica harbours some of the most pristine marine environments remaining, but is increasingly vulnerable to anthropogenic pressures, climate change, and invasion by non-native species. Monitoring biotic responses cumulative impacts requires temporal spatial baselines ongoing monitoring - traditionally, this has been obtained continuous plankton recorder (CPR) surveys. Here, we conduct one longest environmental DNA (eDNA) transects yet, spanning over 3000 nautical miles from Hobart (Australia) Davis Station (Antarctica). We evaluate eDNA sampling strategies for long-term open ocean biomonitoring comparing two water volume filter pore size combinations: large (12 l with 20 μm) small (2 0.45 μm). Employing a broad COI metabarcoding assay, found sample/pore combination was better suited monitoring, detecting more target rare or low abundance Comparisons four simultaneously conducted CPR revealed that detections were diverse than CPR, 7 (4 unique) 4 (1 phyla respectively. While both methods effectively delineated biodiversity patterns across Ocean, enables surveys in presence sea-ice where cannot be conducted. Accordingly, 16 species concern detected along transect using eDNA, notably Antarctic region (south 60°S). These largely attributed hull biofouling, recognized pathway introductions into Antarctica. Given vulnerability potential warming work underscores importance continued biosecurity vigilance. advocate integrating emphasising urgency its implementation. anticipate interweaving biophysical data will generate nuanced picture ecosystems, significant implications conservation preservation ecosystems.

Язык: Английский

Evaluating zooplankton species diversity using environmental DNA and bulk-DNA metabarcoding in the Ulleung Basin of the Southeastern Korean Peninsula in the summer

Frontiers in Marine Science, Год журнала: 2024, Номер 11

Опубликована: Окт. 28, 2024

Accurately investigating the composition of zooplankton species is crucial for monitoring changes in marine ecosystems and assessing biodiversity. In this study, we utilized bulk DNA environmental metabarcoding Ulleung Basin, known its high diversity among seas surrounding Korean Peninsula. Genomic extracted from samples collected during three summer seasons survey area was analyzed using high-throughput sequencing cytochrome c oxidase I barcode region. We identified 350 species, which were to six times more than those by traditional morphological methods. Furthermore, observed significant differences between eDNA samples. Notably, effectively detected with swimming ability that difficult capture sampling This study underscores impact methods on research outcomes studies highlights importance integrating different techniques. Specifically, it suggests need active adoption non-invasive methods, such as metabarcoding, comprehensive diverse biological groups ecosystems.

Язык: Английский