Limosilactobacillus Regulating Microbial Communities to Overcome the Hydrolysis Bottleneck with Efficient One‐Step Co‐Production of H₂ and CH₄

Advanced Science, Год журнала: 2024, Номер unknown

Опубликована: Сен. 12, 2024

Abstract The efficient co‐production of H 2 and CH 4 via anaerobic digestion (AD) requires separate stages, as it cannot yet be achieved in one step. Lactic acid bacteria (LAB) ( Limosilactobacillus ) release acetate by enhancing hydrolysis, potentially increasing production with simultaneous accumulation. This study investigated the enhanced effect one‐step AD LAB elucidated its enhancement mechanisms. results showed that 236.3 times increase 7.1 are achieved, resulting profits 469.39 USD. Model substrates lignocellulosic straw, sodium acetate, confirmes work on hydrolysis stage subsequent sustainable volatile fatty during first 6 days AD. In this stage, enrichment carrying bglB xynB , glycolysis pathway, high activity protease, kinase, [FeFe] hydrogenase, jointly rapid accumulation, driving hydrogenotrophic methanogenesis dominated. From day 7 to 24, enriched Methanosarcina increased methenyltetrahydromethanopterin hydrogenase activity, continuously produced led mainly acetoclastic shift from methanogenesis. power generation capacity LAB‐enhanced is 333.33 China's 24,000 m 3 biogas plant.

Язык: Английский

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New Horizons in Micro/Nanoplastic-Induced Oxidative Stress: Overlooked Free Radical Contributions and Microbial Metabolic Dysregulations in Anaerobic Digestion

Environmental Science & Technology, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 5, 2024

Excessive production of reactive oxygen species (ROS) induced by micro/nanoplastics (MPs/NPs) is highly toxic to microbes. However, the mechanisms underlying ROS generation and metabolic regulation within anaerobic guilds remain poorly understood. In this study, we investigated effects environmentally relevant levels polypropylene (PP)-MPs/NPs on oxidative stress microbial ecology during digestion (AD). Electron paramagnetic resonance spectroscopy revealed that PP-MPs/NPs elevated concentrations persistent free radicals (EPFRs) derived hydroxyl (•OH). EPFRs were identified as primary contributors •OH generation, evidenced a high Spearman correlation coefficient (r = 0.884, p < 0.001) radical-quenching studies. The formation enhanced 86.2–100.9%, resulting in decreased cellular viability methane (by 37.5–50.5%) at 100 mg/g TS PP-MPs/NPs. Genome-centric metagenomic metatranscriptomic analyses suggested reassembly community structures, re-evolution functional traits, remodeling interspecies interactions. Specifically, shift methanogen consortia from hydrogenotrophic Methanofollis sp. acetoclastic Methanothrix soehngenii, primarily because latter's diverse ingestion patterns, electron bifurcation complexes, ROS-scavenging abilities. Downregulation genes associated with antioxidative defense systems (i.e., sodN, katA, osmC) ROS-driven redox signal transduction pathways (c-di-AMP phosphorylation signaling pathways) provided insights into ROS-induced dysregulation. Our findings enhance understanding ecological traits under MPs/NPs stressors, facilitating control toxicity stabilization AD processes.

Язык: Английский

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Effects of alkaline pretreatment on the distribution of amino acid enantiomers and their biofunctions during the anaerobic digestion of waste activated sludge

Chemical Engineering Journal, Год журнала: 2025, Номер 505, С. 159334 - 159334

Опубликована: Янв. 9, 2025

Язык: Английский

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A review of temperature and key parameters influencing the hydrolysis-methanogenesis balance in anaerobic digestion

Fuel, Год журнала: 2025, Номер 394, С. 134927 - 134927

Опубликована: Март 22, 2025

Язык: Английский

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Design and Performance Evaluation of Small-Scale Biogas Digesters Using Locally Available Materials in Rural Uganda

Renewable Energy, Год журнала: 2025, Номер unknown, С. 122994 - 122994

Опубликована: Март 1, 2025

Язык: Английский

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Enhanced bioavailability of trace elements for improving anaerobic digestion of food waste using sludge extracellular polymeric substances

Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 163589 - 163589

Опубликована: Май 1, 2025

Язык: Английский

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Synergistic effects of anaerobic digestion for diverse feedstocks: A holistic study on feedstock properties, process efficiency, biogas yield, and economic viability

Energy Sustainable Development/Energy for sustainable development, Год журнала: 2025, Номер 87, С. 101755 - 101755

Опубликована: Май 20, 2025

Язык: Английский

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Changes in anaerobic digestion performance and microbial community by increasing SRT through sludge recycling in food waste leachate treatment

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Июнь 5, 2025

Язык: Английский

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Syntrophic Relationship Among Microbial Communities Enhance Methane Production During Temperature Transition from Mesophilic to Thermotolerant Conditions

Journal of environmental chemical engineering, Год журнала: 2024, Номер unknown, С. 114903 - 114903

Опубликована: Ноя. 1, 2024

Язык: Английский

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Effect of Temperature on the Inocula Preservation, Mesophilic Anaerobic Digestion Start-Up, and Microbial Community Dynamics

Agronomy, Год журнала: 2024, Номер 14(12), С. 2991 - 2991

Опубликована: Дек. 16, 2024

Anaerobic digestion (AD) is a well-established technology for the sustainable conversion of agricultural organic by-products and waste into bioenergy. Temperature crucial optimizing methane production through inocula preservation reactor start-up in AD. The induced by temperature has rarely been assessed from an engineering perspective. There also limited exploration influence high-to-moderate transition on initiation This study employed continuous mesophilic AD reactors with potential applications to conduct revival tests. These tests evaluated activity sludge stored at different temperatures investigated impact high-temperature Additionally, we elucidated correlation between these assessments microbial diversity as well composition. results indicated that bacterial was higher inoculum 35 °C compared 15 °C, ensuring stable operation richness bacteria archaea remained during high temperatures. conducive enhancing methanogenic initiated 55 °C. continuously operated system showed significant differences composition its inoculum. Increased abundance Coriobacteriaceae Prevotellaceae led propionate butyrate accumulation, respectively, reducing operational capacity. Methanogenic were less diverse low-temperature preserved medium temperature. Streptococcaceae temperarure could promote stability. Hydrogenotrophic methanogens had competitive advantage due their prior exposure initiation, possibly influenced Thermotogaceae.

Язык: Английский

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