bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Окт. 14, 2023
Abstract
Biological
cryopreservation
often
involves
using
a
cryoprotective
agent
(CPA)
to
mitigate
lethal
physical
stressors
cells
endure
during
freezing
and
thawing,
but
effective
CPA
concentrations
are
cytotoxic.
Hence,
natural
polysaccharides
have
been
studied
as
biocompatible
alternatives.
Our
current
investigation
26
potential
CPA,
probing
correlations
between
post-thaw
metabolic
viability
(PTV)
of
cryopreserved
Vero
monomeric
composition.
The
best
performing
contained
significant
fucose
amounts,
resulting
in
average
PTV
2.8-fold
(up
3.1-fold)
compared
0.8-fold
2.2-fold
for
all
non-cryoprotective
polysaccharides,
respectively,
outperforming
the
optimized
commercial
CryoStor™
CS5
formulation
(2.6-fold).
Stoichiometrically,
balance
(18–35.7
mol%),
uronic
acids
(UA)
(13.5–26
mol%)
high
molecular
weight
(MW
>
1
MDa)
generated
optimal
PTV.
To
deconvolute
multiple
variable
effects,
principal
component
analysis
(PCA)
coupled
K
-means
clustering
was
performed.
Two
major
mechanisms
action
explained
variability:
charge-dependent
effect
contrasting
charged
acid
neutral
monomer
compositions,
MW-scaled
charge-independent
mechanism
exclusively
attributed
fucose.
Ultimately,
our
research
showed
critical
role
plays
enhancing
cellular
outcomes,
disputing
previous
assumptions
polyanionicity
being
sole
governing
predictor
cryoprotection,
highlighting
fucose-rich
polyanionic
polysaccharides.
Research Square (Research Square),
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 4, 2024
Abstract
Levan,
an
exopolysaccharide,
has
applications
in
the
medical
and
food
sectors.
Under
natural
conditions,
levan
is
produced
at
a
low
concentration
by
microorganisms.
This
current
study
focuses
on
optimizing
production
one-variable-at-a-time
approach
from
novel
marine
bacterium,
Calidifontibacillus
erzurumensis
LEV207.
The
growth
levansucrase
C.
LEV207
were
studied
to
optimize
media
components
conditions.
Sucrose
yeast
extract
proved
be
good
inducer.
pH
of
6.0
temperature
30°C,
along
with
MgSO4
as
salt,
increased
enzyme
activity.
50%
1%,
respectively,
maximum
activity
427.4
µmol/mL/min
after
48
hrs
incubation.
optimization
process
(EPSLEV207)
7-fold
final
yield
65.58
g/L.
To
confirm
structure
nature
EPSLEV207,
NMR
XRD
performed,
functional
groups
present
EPSLEV207
determined
using
FTIR.
Chemical
hydrolysis
confirmed
fructose
monomer,
making
homopolysaccharide.
exhibited
49%
22%
radical
scavenging
against
DPPH
ABTS
radicals,
promoted
probiotic
strain.
showed
88%
water
solubility
index
78%
retention
capacity.
up
7-fold,
highlighting
potential
viable
option
for
industrial
applications.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 16, 2024
Abstract
The
aim
of
this
study
was
to
explore
the
viability
dual
production
polyhydroxybutyrate
(PHB)
and
exopolysaccharides
(EPS)
by
seven
microbiomes
rich
in
cyanobacteria.
Our
initial
experiments
involved
screen
for
EPS-producing
candidates
examine
impact
salinity
acetate
on
EPS
synthesis.
Salinity’s
known
influence
acetate’s
role
enhancing
PHB
guided
our
parameter
selection.
Surprisingly,
neither
introduction
an
external
carbon
source
(acetate)
nor
exposure
abiotic
stressor
(salt)
significantly
altered
synthesis
rates,
which
ranged
from
25
150
mg·L
-1
,
or
its
composition,
with
glucose
being
dominant
sugar
component.
Scaling
up
a
3
L
photobioreactor,
we
achieved
simultaneous
biopolymer
production,
reaching
205
87
PHB.
Additionally,
presence
uronic
acid
facilitated
biomass
flocculation,
streamlining
separation
process,
potentially
reducing
associated
time
costs.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Окт. 14, 2023
Abstract
Biological
cryopreservation
often
involves
using
a
cryoprotective
agent
(CPA)
to
mitigate
lethal
physical
stressors
cells
endure
during
freezing
and
thawing,
but
effective
CPA
concentrations
are
cytotoxic.
Hence,
natural
polysaccharides
have
been
studied
as
biocompatible
alternatives.
Our
current
investigation
26
potential
CPA,
probing
correlations
between
post-thaw
metabolic
viability
(PTV)
of
cryopreserved
Vero
monomeric
composition.
The
best
performing
contained
significant
fucose
amounts,
resulting
in
average
PTV
2.8-fold
(up
3.1-fold)
compared
0.8-fold
2.2-fold
for
all
non-cryoprotective
polysaccharides,
respectively,
outperforming
the
optimized
commercial
CryoStor™
CS5
formulation
(2.6-fold).
Stoichiometrically,
balance
(18–35.7
mol%),
uronic
acids
(UA)
(13.5–26
mol%)
high
molecular
weight
(MW
>
1
MDa)
generated
optimal
PTV.
To
deconvolute
multiple
variable
effects,
principal
component
analysis
(PCA)
coupled
K
-means
clustering
was
performed.
Two
major
mechanisms
action
explained
variability:
charge-dependent
effect
contrasting
charged
acid
neutral
monomer
compositions,
MW-scaled
charge-independent
mechanism
exclusively
attributed
fucose.
Ultimately,
our
research
showed
critical
role
plays
enhancing
cellular
outcomes,
disputing
previous
assumptions
polyanionicity
being
sole
governing
predictor
cryoprotection,
highlighting
fucose-rich
polyanionic
polysaccharides.
