Dry
eye
disease
(DED)
is
a
multifactorial
condition
characterized
by
ocular
surface
inflammation,
tear
film
instability,
and
corneal
epithelial
damage.
Current
treatments
often
provide
temporary
relief
without
addressing
the
underlying
inflammatory
mechanisms.
This
study
examined
therapeutic
potential
of
crocin
nobiletin,
two
naturally
derived
compounds
with
well-known
antioxidant
anti-inflammatory
properties,
in
mouse
model
DED
induced
lacrimal
gland
excision
(LGE).
Thirty
female
Balb/c
mice
were
divided
into
five
groups
(n
=
6
each):
Control
(sham
surgery),
untreated
DED,
nobiletin-treated
(32.75
µM),
crocin-treated
(34
1%
betamethasone-treated
DED.
Treatments
administered
three
times
daily
for
28
days.
Ocular
tissues
evaluated
using
Hematoxylin
Eosin
(H&E)
staining
fluorescein
staining.
Conjunctival
cytokines,
including
interleukin-6
(IL-6),
interleukin-1
beta
(IL-1β),
tumor
necrosis
factor-alpha
(TNF-α),
measured
enzyme-linked
immunosorbent
assay
(ELISA).
Histological
analysis
showed
that
nobiletin
treatment
exhibited
reduced
disruption,
keratinization,
cell
infiltration
compared
to
group.
The
ELISA
revealed
both
efficiently
inhibited
production
pro-inflammatory
cytokines
IL-6,
TNF-α,
IL-1β,
which
are
key
mediators
pathogenesis.
Fluorescein
further
confirmed
protective
impact
on
integrity.
Moreover,
epithelial-preserving
effects
these
comparable
those
corticosteroid
betamethasone.
Overall,
findings
suggest
have
management
modulating
responses
enhancing
healing.
These
offer
promising
avenues
development
safer
more
effective
this
challenging
condition.
However,
investigations,
clinical
trials,
essential
elucidate
mechanisms
action
optimize
approaches.
Molecules,
Год журнала:
2023,
Номер
28(9), С. 3705 - 3705
Опубликована: Апрель 25, 2023
Matrix
metalloproteinase-9
(MMP-9),
one
of
the
most
investigated
and
studied
biomarkers
MMPs
family,
is
a
zinc-dependent
proteolytic
metalloenzyme
whose
primary
function
degrading
extracellular
matrix
(ECM).
It
has
been
proved
that
MMP-9
expression
elevates
in
multiple
pathological
conditions,
including
thyroid
carcinoma.
detectable
higher
level
malignant
or
metastatic
tumor
tissues
than
normal
benign
acts
as
an
additional
marker
to
distinguish
different
stages
because
its
close
correlations
with
clinical
features,
such
lymph
node
metastasis,
TNM
stage,
size
so
on.
Natural
non-natural
inhibitors
suppress
expression,
block
progression
diseases,
play
role
therapy
consequently.
inhibitory
molecules
also
assist
treating
tumors
by
suppressing
proliferation,
invasion,
migration,
viability,
adhesion,
motility,
epithelial-mesenchymal
transition
(EMT),
other
risk
factors
cancer
cells.
In
word,
discovering
designing
provide
great
therapeutic
effects
promising
values
various
types
International Journal of Molecular Sciences,
Год журнала:
2022,
Номер
23(8), С. 4256 - 4256
Опубликована: Апрель 12, 2022
Eye
diseases
are
associated
with
visual
impairment,
reduced
quality
of
life,
and
may
even
lead
to
vision
loss.
The
efficacy
available
treatment
eye
is
not
satisfactory.
unique
environment
the
related
anatomical
physiological
barriers
constraints
limits
bioavailability
existing
agents.
In
turn,
complex
ethiopathogenesis
ocular
disorders
that
used
drugs
generally
non-disease
specific
do
act
causally.
Therefore,
there
a
need
for
development
new
therapeutic
preventive
approach.
It
seems
matrix
metalloproteinases
(MMPs)
tissue
inhibitors
(TIMPs)
have
significant
role
in
progression
could
be
therapy
these
as
pharmacological
targets.
MMPs
TIMPs
play
an
important
angiogenesis,
epithelial-mesenchymal
transition,
cell
invasion,
migration,
which
occur
diseases.
this
review,
we
aim
describe
participation
diseases,
such
age-related
macular
degeneration,
cataract,
diabetic
retinopathy,
dry
syndrome,
glaucoma,
cancers,
posterior
capsule
opacification
focusing
on
potential
mechanisms.
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(2), С. 1458 - 1458
Опубликована: Янв. 11, 2023
Dry
eye
disease
(DED)
is
a
multifactorial
condition
resulting
from
reduced
tear
secretion
the
lacrimal
glands,
increased
water
evaporation
or
production
of
poor-quality
tears.
Such
instability
can
lead
to
inflammation
and
damage
ocular
surface,
as
well
abnormal
nociception.
Historically,
substitutes
corticosteroids
have
been
bastion
DED
therapy,
but
substantial
number
patients
still
suffer
residual
symptoms
even
after
being
treated
with
traditional
treatments.
Aiming
find
safe
effective
alternative
therapies,
recent
efforts
focused
on
role
vitamin
D
in
cellular
physiology
eye.
Possibly
because
its
positive
effect
modulating
immune
inflammatory
responses,
systemic
supplementation
seems,
indeed,
be
an
therapeutic
strategy,
especially,
not
only,
for
affected
by
that
does
respond
conventional
In
this
context,
review
focuses
literature
reporting
pathogenesis
treatment
DED,
special
emphasis
investigations
potential
administration
approach
management
such
condition.
Scientific Reports,
Год журнала:
2023,
Номер
13(1)
Опубликована: Авг. 11, 2023
Dry
eye
syndrome
(DES)
is
a
chronic
ocular
disease
that
induces
epithelial
damage
to
the
cornea
by
decreasing
tear
production
and
quality.
Adequate
treatment
options
have
not
been
established
for
severe
DES
such
as
Sjogren's
due
complicated
pathological
conditions.
To
solve
this
problem,
we
focused
on
conditioned
medium
of
human
adipose-derived
mesenchymal
stem
cells
(hAdMSC-CM),
which
multiple
therapeutic
properties.
Here,
showed
hAdMSC-CM
suppressed
Benzalkonium
Chloride
(BAC)-induced
cytotoxicity
inflammation
in
corneal
(hCECs).
In
addition,
increased
expression
level
regulated
localisation
barrier
function-related
components,
improved
BAC-induced
dysfunction
hCECs.
RNA-seq
analysis
pharmacological
inhibition
experiments
revealed
effects
were
associated
with
TGFβ
JAK-STAT
signalling
pathways.
Moreover,
model
rats
exorbital
intraorbital
lacrimal
gland
excision,
instillation
improving
cornea.
Thus,
demonstrated
has
properties
pathways,
may
serve
an
innovative
agent
function.
Journal of Neural Transmission,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 4, 2025
Abstract
Botulinum
toxin
type
A
(BoNT)
has
emerged
as
a
potential
alternative
to
conventional
therapies
many
debilitating
chronic
diseases
characterised
by
inflammatory
states.
However,
the
biological
rationale
remains
ambiguous.
Our
review
aimed
systematically
assessed
which
biochemical
biomarkers
have
been
reported
in
clinical
research
evaluate
BoNT
analgesic
and
mood-lifting
effects
head
neck
conditions
related
inflammation.
We
searched
databases
registries
between
inception
September
29,
2023.
Of
nine
included
studies,
there
were
concerns
about
risk
of
bias
for
six
studies.
The
leading
biomarker
with
five
studies
was
calcitonin
gene-related
peptide
(CGRP),
followed
serotonin
two
Oxidative
stress
only
one
study.
Several
important
players
processes
different
immune
cell
classes
evaluated
four
There
trial
measuring
changes
beta
Tubulin
SNAP-25,
another
study
evaluating
cutaneous
neuropeptide
substance-P.
After
BoNT,
significant
effect
including
decrease
plasma
levels
CGRP
migraine
trigeminal
neuralgia;
when
collected
from
human
tears
refractory
intractable
dry
eye
disease
increase
peripheral
blood
platelets
painful
cervical
dystonia
associated
depression
anxiety;
concentration
markers
oxidative
damage
proteins
antioxidant
power;
expression
gene
sets
involved
pathways
cells
periosteum
metalloproteinase-9
molecule
tears.
seems
affect
some
present
conditions.
certainty
evidence
found
very
low
moderate.
This
is
registered
on
PROSPERO
(CRD42023432131).
Nature Communications,
Год журнала:
2022,
Номер
13(1)
Опубликована: Март 11, 2022
Abstract
The
insights
into
how
genome
topology
couples
with
epigenetic
states
to
govern
the
function
and
identity
of
corneal
epithelium
are
poorly
understood.
Here,
we
generate
a
high-resolution
Hi-C
interaction
map
human
limbal
stem/progenitor
cells
(LSCs)
show
that
chromatin
multi-hierarchical
organisation
is
coupled
gene
expression.
By
integrating
Hi-C,
epigenome
transcriptome
data,
characterize
comprehensive
3D
epigenomic
landscapes
LSCs.
We
find
super-silencers
mediate
repression
associated
development,
differentiation
disease
via
looping
and/or
proximity.
Super-enhancer
(SE)
analysis
identified
set
SE
interactive
hubs
contribute
LSC-specific
activation.
These
active
inactive
element-anchored
loop
networks
occur
within
cohesin-occupied
CTCF-CTCF
loops.
further
reveal
coordinated
regulatory
network
core
transcription
factors
based
on
SE-promoter
interactions.
Our
results
provide
detailed
organization
principle
for
regulation
expression
in
stratified
epithelia.
Molecules,
Год журнала:
2024,
Номер
29(16), С. 3732 - 3732
Опубликована: Авг. 7, 2024
Dry
eye
syndrome
(DES)
is
a
dynamic,
chronic
disease
of
the
ocular
surface
and
appendages
caused
by
inflammation.
The
most
common
symptoms
include
redness,
itching,
blurred
vision,
resulting
from
dysfunction
meibomian
glands
impaired
tear-film
production.
Factors
contributing
to
development
DES
environmental
elements,
such
as
UV
radiation,
internal
hormonal
imbalances.
These
factors
increase
oxidative
stress,
which
exacerbates
inflammation
on
accelerates
DES.
In
recent
years,
incidence
has
risen,
leading
greater
need
develop
effective
treatments.
Current
treatments
for
dry
are
limited
primarily
focus
alleviating
individual
symptoms,
reducing
surface.
However,
it
crucial
understand
pathomechanism
tailor
treatment
address
underlying
causes
achieve
best
possible
therapeutic
outcomes.
Therefore,
in
this
review,
we
analyzed
impact
stress
gain
better
understanding
its
examined
recently
developed
nanosystems
that
allow
drugs
be
delivered
directly
site.
