Effects of Cathepsin S Inhibition in the Age-Related Dry Eye Phenotype

Investigative Ophthalmology & Visual Science, Год журнала: 2023, Номер 64(11), С. 7 - 7

Опубликована: Авг. 4, 2023

Aged C57BL/6J (B6) mice have increased levels of cathepsin S, and aged S (Ctss-/-) knockout are resistant to age-related dry eye. This study investigated the effects inhibition on eye disease.

Язык: Английский

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Proteases and Their Potential Role as Biomarkers and Drug Targets in Dry Eye Disease and Ocular Surface Dysfunction

International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(17), С. 9795 - 9795

Опубликована: Авг. 29, 2022

Dry eye disease (DED) is a multifactorial disorder that leads to ocular discomfort, visual disturbance, and tear film instability. DED accompanied by an increase in osmolarity surface inflammation. The diagnosis treatment of still present significant challenges. Therefore, novel biomarkers treatments are great interest. Proteases different tissues on the surface. In healthy eye, proteases highly regulated. However, dysregulation occurs various pathologies, including DED. With this review, we provide overview implications families development severity DED, along with studies involving protease inhibitors as potential therapeutic tools. Even though further research needed, review aims give suggestions for identifying developing new inhibitors.

Язык: Английский

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Short-term exposure to cigarette smoke upregulates cathepsin S and alters expression of tight junction ZO-1

Biochimie, Год журнала: 2024, Номер 226, С. 29 - 34

Опубликована: Март 15, 2024

Язык: Английский

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Restoration of corneal epithelial barrier function: A possible target for corneal neovascularization

The Ocular Surface, Год журнала: 2024, Номер 34, С. 38 - 49

Опубликована: Июнь 18, 2024

Язык: Английский

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Inhibition of Cathepsin S in Autoimmune CD25KO Mouse Improves Sjögren Disease–Like Lacrimal Gland Pathology

Investigative Ophthalmology & Visual Science, Год журнала: 2024, Номер 65(8), С. 26 - 26

Опубликована: Июль 17, 2024

Purpose: CD25KO mice are a model of Sjögren disease (SjD) driven by autoreactive T cells. Cathepsin S (CTSS) is protease crucial for major histocompatibility complex class II presentation that primes We investigated if diet containing CTSS inhibitor would improve autoimmune signs in mice. Methods: Four-week female were randomly chosen to receive chow (R05461111, 262.5 mg/kg chow) or standard 4 weeks. Cornea sensitivity was measured. Inflammatory score assessed lacrimal gland (LG) histologic sections. Flow cytometry LG and ocular draining lymph nodes (dLNs) expression Th1 Th17 Expression inflammatory, T- B-cell, apoptotic markers the with quantitative PCR. The life span receiving compared. CD4+ cells from both groups isolated spleens adoptively transferred into RAG1KO recipients. Results: Mice had better cornea improved inflammatory scores. There significant decrease frequency immune increase CD8+ dLNs LGs dLNs. Ifng, Ciita, Casp8 mRNA decreased. received lived 30% longer. Adoptive transfer recipients inhibitor-treated lower inflammation Conclusions: Inhibiting could be potential venue treatment SjD eye LG.

Язык: Английский

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Heterochronic Parabiosis Causes Dacryoadenitis in Young Lacrimal Glands

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(5), С. 4897 - 4897

Опубликована: Март 3, 2023

Aging is associated with inflammation and oxidative stress in the lacrimal gland (LG). We investigated if heterochronic parabiosis of mice could modulate age-related LG alterations. In both males females, there were significant increases total immune infiltration isochronic aged LGs compared to that young LGs. Male significantly more infiltrated male While females had inflammatory B-cell-related transcripts levels LGs, a greater fold expression some these than males. Through flow cytometry, specific subsets B cells increased those Our results indicate serum soluble factors from not enough reverse infiltrating tissues sex-related differences treatment. This suggests changes microenvironment/architecture participate perpetuating inflammation, which reversible by exposure youthful systemic factors. contrast, worse their counterparts, suggesting can enhance host. Therapies aim at improving cellular health may have stronger impact on parabiosis.

Язык: Английский

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Conditional deletion of CD25 in the corneal epithelium reveals sex differences in barrier disruption

The Ocular Surface, Год журнала: 2023, Номер 30, С. 57 - 72

Опубликована: Июль 27, 2023

Язык: Английский

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Cysteine Cathepsins and Their Diagnostic Role in Breast Cancer

Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology/Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology, Год журнала: 2024, Номер 16(1), С. 1 - 14

Опубликована: Янв. 10, 2024

Background: A large class of proteolytic enzymes known as cysteine cathepsins are created pro-enzymes and activated in slightly acidic conditions.They crucial for both healthy cellular function illness.Cysteine a family proteases that play important roles variety processes, including protein turnover, cell adhesion, migration.Main body: In breast cancer, have been shown to be overexpressed tumor cells associated with poor prognosis.This review article discusses the role focusing on their diagnostic potential.The provides an overview cathepsins, structure, function, regulation addition evidence overexpression cancer association prognosis.Conclusions: The current demonstrates potential use biomarkers early detection diagnosis cancer.

Язык: Английский

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EDITORIAL: Special issue on the Tear Film and the Ocular Surface

Experimental Eye Research, Год журнала: 2023, Номер 238, С. 109705 - 109705

Опубликована: Ноя. 7, 2023

Язык: Английский

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